Results 221 to 230 of about 166,603 (275)

Protocol to clone unknown flanking genomic region using SWPOP-PCR for genome walking. [PDF]

STAR Protoc
Tang Q, Chang K, Li M, Li H.
europepmc   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, EarlyView.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Enhancing species discovery and description in algal turfs: A case study in the green alga Pseudoderbesia (Bryopsidales)

Journal of Phycology, EarlyView.
Abstract Algal turfs are assemblages consisting of small marine green, brown, and red algae on the scale of millimeters to a few centimeters. Due to their small size, they have been less intensively studied by macroalgal taxonomists, and they also fall outside the scope of microalgal taxonomists, who tend to focus on smaller, often unicellular, taxa ...
Amelia Hastings, Chiela Cremen, Myles Courtney, Yuqun Du, Heroen Verbruggen   +4 more
wiley   +1 more source

Filter feeders living on suspension feeders: New insights into the lifestyle and distribution of Arcturidae Dana, 1849 (Crustacea: Isopoda) around Iceland

Marine Ecology, EarlyView.
Abstract The focus of the present study was on arcturid isopods occurring in the subarctic region around Iceland. Data from two decades of sampling with an epibenthic sledge (EBS) during numerous expeditions of the BIOICE (Benthic Invertebrates of Icelandic Waters; 1992–2004) and IceAGE (Icelandic marine Animals: Genetics and Ecology; 2011—ongoing ...
Vivien Lukas Hartmann, Emanuel Pereira, Nancy F. Mercado‐Salas, Anne‐Nina Lörz, Jörundur Svavarsson, Saskia Brix   +5 more
wiley   +1 more source

Expanded Functional Characterization and Optimization of Protein Expression in Treponema denticola Shuttle Plasmids

Molecular Oral Microbiology, EarlyView.
We report optimization of the Treponema denticola shuttle plasmid system by minimizing its size and by characterizing an array of promoter‐gene constructs for genetic complementation, including the first use in Treponema of a Tet‐inducible system for controlled expression of potentially toxic genes.
M. Paula Goetting‐Minesky, Valentina Godovikova, Prakaimuk Saraithong, Alexander H. Rickard, Brigette R. Crawley, Sara M. Agolli, Reagan L. Boyce, Trishna L. Appaji, J. Christopher Fenno   +8 more
wiley   +1 more source

MsWRKY49 Modulates Pollen Tube Elongation in Response to Boron Deficiency by Regulating Reactive Oxygen Species Homeostasis in Alfalfa

Plant Biotechnology Journal, EarlyView.
ABSTRACT Boron, an essential micronutrient, plays a crucial role in plant reproductive processes. Boron deficiency is widespread in many regions and significantly reduces alfalfa (Medicago sativa) seed yield, yet the molecular mechanisms underlying its impact on reproductive organ development remain elusive.
Juan Wang, Wenxin Peng, Zhicheng Jia, Xinru Su, Tianqi Zhu, Zhenzhen Liu, Shoujiang Sun, Jinyu Shi, Manli Li, Liru Dou, Peisheng Mao   +10 more
wiley   +1 more source

Rosmarinic Acid Synthase 1 Phosphorylation by SmMAPK3 Is Required for Salicylic Acid‐Induced Salvianolic Acid Accumulation in Salvia miltiorrhiza Hairy Roots

Plant Biotechnology Journal, EarlyView.
ABSTRACT Salvianolic acid is the main active component of Salvia miltiorrhiza and holds significant value in the clinical treatment of myocardial ischemia and hypoxia. Previous studies have shown that salicylic acid (SA) can significantly promote the accumulation of salvianolic acid, but its molecular mechanism remains incompletely understood.
Xuecui Yin, Shuang Liu, Yuhang Zhang, Wenjing Yang, Junwen Bu, Bin Zhang, Juane Dong   +6 more
wiley   +1 more source

TaSPX3 Enhances Wheat Resistance to Leaf Rust by Antagonising TaDi19‐Mediated Repression of Pathogenesis‐Related Genes

Plant Biotechnology Journal, EarlyView.
ABSTRACT Wheat leaf rust, caused by Puccinia triticina (Pt), threatens global wheat production, with yield losses further exacerbated by the pathogen's evolving virulence. Although Syg1/Pho81/Xpr1 (SPX) domain‐containing proteins are known regulators of phosphate homeostasis, their involvement in plant–pathogen interactions remains largely unexplored ...
Huimin Qian, Chuang Li, Yanan Lu, Xue Li, Jianping Zhang, Junyi Zhao, Keyan Wu, Yanyan Zhang, Kun Cheng, Daowen Wang, Pengyu Song, Na Liu, Wenming Zheng   +12 more
wiley   +1 more source

Artificial Mutations in the Nuclear Gene Encoding Mitochondrial RNA Polymerase Restore Pollen Fertility in Cytoplasmic Male Sterile Tomato

Plant Biotechnology Journal, EarlyView.
ABSTRACT Cytoplasmic male sterility (CMS) and restorer of fertility (Rf) are important traits in F1 hybrid breeding. However, the CMS/Rf system has not been used in tomatoes because of the limited resources of Rf lines. In this study, we performed mutagenesis in tomato CMS seeds and successfully obtained 13 suppressor mutants with pollen fertility ...
Kosuke Kuwabara, Rika Nakajima, Van Bosstraeten Alexis Gaetan, Kentaro Ezura, Kinya Toriyama, Tohru Ariizumi, Kenta Shirasawa   +6 more
wiley   +1 more source

Moderate Regulation of SBEIIb Gene Expression by Editing Cis‐Regulatory Elements Enhances the Resistant Starch Content in Rice

Plant Biotechnology Journal, EarlyView.
ABSTRACT As the staple food of over half the world's population, rice with a high resistant starch (RS) content may be beneficial in the dietary prevention of diabetes and hyperlipidemia. Mutating key enzymes in starch metabolism to increase the RS content has been successful in other crops.
Qingqing Yang, Ying Ye, Jiannan Zhu, Yan Tan, Dongsheng Zhao, Qiaoquan Liu   +5 more
wiley   +1 more source