Results 21 to 30 of about 666,195 (194)
BioTechniques, 1996 The Applied Biosystems PRISMTM fluorescence-based genotyping system as well as the Invitrogen TA Cloning® vector system are influenced by the tendency of Taq DNA polymerase to add an adenine nucleotide to the 3′ end of PCR products after extension ...
V.L. Magnuson +8 more
doaj +2 more sources
Optimization of Taq DNA polymerase enzyme expression in Escherichia coli
Advanced Biomedical Research, 2012 Background: In the present study, we optimized the experimental conditions using pET15b expression vector to obtain large amounts of Taq DNA polymerase.
Fateme Moazen +5 more
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Increased efficiency of the Taq polymerase catalyzed polymerase chain reaction [PDF]
Nucleic Acids Research, 1989 Stephen A. Krawetz +2 more
openalex +5 more sources
Comparison between Taq DNA Polymerase and Its Stoffel Fragment for Quantitative Real-Time PCR with Hybridization Probes [PDF]
BioTechniques, 2001 In quantitative real-time PCR assays, fluorophor-labeled oligonucleotide probes are employed to generate sequence-specific signals for the quantitative evaluation. Whereas TaqMan® probes have to be hydrolyzed during PCR by the endonucleolytic activity of
Jochen Wilhelm +2 more
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Taq polymerase contains bacterial DNA of unknown origin
Molecular and Cellular Probes, 1990 The polymerase chain reaction (PCR) was carried out with the highly conserved E. coli ribosomal RNA gene sequences 1376-1395 and 1521-1540. Using these primers and reaction conditions specified by the manufacturer(s), a 165 bp fragment was synthesized using Taq polymerase from three different sources in the absence of any added template.
Department of Pathology University of Florida Gainesville, Florida, U.S.A. ( host institution ) +2 more
openaire +5 more sources
Replication of cyclobutane pyrimidine dimer analogue by Ex Taq DNA polymerase
Science and Technology of Advanced Materials, 2007 We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine (CVU)-containing ODN at the 5'-terminal.
Masayuki Ogino et al
doaj +2 more sources
PCR methods and applications, 1992 The 3'-->5' exonuclease activity of Vent, a thermostable polymerase from Thermococcus litoralis, enhances DNA replication fidelity but also diverts PCR primers (amplimers) from targeted amplification by degrading their 3' termini.
C M de Noronha, James I. Mullins
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Frequent contamination of Taq polymerase with DNA
Clinical Chemistry, 1990 E. Böttger
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Post-PCR Labeling Using Taq DNA Polymerase
BioTechniques, 1997 Zhidong Chen, Duane E. Ruffner
doaj +4 more sources