Results 21 to 30 of about 819,635 (326)

Data of expression and purification of recombinant Taq DNA polymerase

Data in Brief, 2016
Polymerase chain reaction (PCR) technique is widely used in many experimental conditions, and Taq DNA polymerase is critical in PCR process. In this article, the Taq DNA polymerase expression plasmid is reconstructed and the protein product is obtained by rapid purification, ("Rapid purification of high-activity Taq DNA polymerase" (Pluthero, 1993 [1]),
Yueyang Yang, Na Fang, Shaoping Ji, Niannian Zhong, Yujian Guo   +4 more
openaire   +4 more sources

AmpliTaq DNA polymerase, FS dye-terminator sequencing: Analysis of peak height patterns [PDF]

BioTechniques, 1996
Taq DNA polymerases in which the phenylalanine is substituted by a tyrosine at position 667 (Taq F667Y) are members of a new class of DNA polymerases that incorporate chain-terminating dideoxyribonucleoside triphosphates (ddNTPs) much more efficiently ...
Deng, Q, Kwok, P. Y., Nickerson, D. A., Parker, L. T., Spurgeon, S, Zakeri, H   +5 more
core   +4 more sources

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

PLoS ONE, 2017
DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR.
Marcin Olszewski, Marta Śpibida, Maciej Bilek, Beata Krawczyk   +3 more
doaj   +2 more sources

A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor [PDF]

Scientific Reports
The DNA polymerase derived from Thermus aquaticus is the most widely utilized among various DNA polymerases, indicating its significant economic importance. Consequently, efforts to achieve a substantial yield of Taq DNA polymerase (Taq-pol) are ongoing.
Fina Amreta Laksmi, Kenny Lischer, Yudhi Nugraha, Wiga Alif Violando, Helbert, Isa Nuryana, Firyal Nida Khasna, Naswandi Nur, Kharisma Panji Ramadhan, Destrianti Adelina Lumban Tobing, Hariyatun, Iman Hidayat   +11 more
doaj   +2 more sources

Coupled protein domain motion inTaqpolymerase revealed by neutron spin-echo spectroscopy

Proceedings of the National Academy of Sciences of the United States of America, 2005
Long-range conformational changes in proteins are ubiquitous in biology for the transmission and amplification of signals; such conformational changes can be triggered by small-amplitude, nanosecond protein domain motion. Understanding how conformational
Zimei Bu, Ralf Biehl, M. Monkenbusch, Dieter Richter, David J.E. Callaway   +4 more
openalex   +2 more sources

A Fusion of Taq DNA Polymerase with the CL7 Protein from Escherichia coli Remarkably Improves DNA Amplification [PDF]

Molecules
DNA polymerases are important enzymes that synthesize DNA molecules and therefore are critical to various scientific fields as essential components of in vitro DNA synthesis reactions, including PCR.
Zhongchen Li, Yaping Wang, Xiangyi Wang, Shuhui Niu, Zhenlong Su, Fei Wang, Jing Ni, Yan Gong, Ben Rao   +8 more
doaj   +2 more sources

Trehalose Is a Potent PCR Enhancer: Lowering of DNA Melting Temperature and Thermal Stabilization of Taq Polymerase by the Disaccharide Trehalose [PDF]

Clinical Chemistry, 2004
Compatible solutes are a class of compounds that stabilize cells and cellular components exposed to extreme conditions. In bacterial systems, the uptake or synthesis of compatible solutes renders the cells and their enzymatic machinery more resistant to ...
Andrej‐Nikolai Spiess, Nadine Mueller, Richard Ivell   +2 more
openalex   +2 more sources

Reverse transcriptase inhibits Taq polymerase activity

Nucleic Acids Research, 1992
Detection of viral RNA by polymerase chain reaction (PCR) requires the prior reverse transcription of the viral RNA. In order to minimise the number of manual manipulations required for processing large numbers of samples, we attempted to design a system whereby all the reagents required for both reverse transcription and amplification can be added to ...
Robert Coelen, Loryn N. Sellner, John S. Mackenzie   +2 more
openaire   +5 more sources

Substrate Nucleotide-Determined Non-Templated Addition of Adenine by Taq DNA Polymerase: Implications for PCR-Based Genotyping and Cloning

BioTechniques, 1996
The Applied Biosystems PRISMTM fluorescence-based genotyping system as well as the Invitrogen TA Cloning® vector system are influenced by the tendency of Taq DNA polymerase to add an adenine nucleotide to the 3′ end of PCR products after extension ...
V.L. Magnuson, D.S. Ally, S.J. Nylund, Z.E. Karanjawala, J.B. Rayman, J.I. Knapp, A.L. Lowe, S. Ghosh, F.S. Collins   +8 more
doaj   +2 more sources

Extreme free energy of stabilization of Taq DNA polymerase

Proteins: Structure, Function, and Bioinformatics, 2004
AbstractWe have examined the chemical denaturations of the Klentaq and Klenow large‐fragment domains of the Type 1 DNA polymerases from Thermus aquaticus (Klentaq) and Escherichia coli (Klenow) under identical solution conditions in order to directly compare the stabilization energetics of the two proteins.
Farheen Khan, Vince J. LiCata, Allison M. Joubert, Chin Chi Liu, Fenggang Peng, Allyn J. Schoeffler   +5 more
openaire   +5 more sources