PCR with Taq DNA Polymerase (M0273) v1
, 2014 This PCR Protocol is for Taq DNA Polymerase with Standard Taq Buffer (M0273)
New England Biolabs
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Engineering of novel DNA polymerase variants for single enzyme quantitative multiplex reverse transcription-PCR [PDF]
Scientific ReportsReverse transcription polymerase chain reaction (RT-PCR) has evolved as a widely used approach in biotechnology and molecular diagnostics. It represents a powerful tool for amplifying and analysing RNA molecules and has therefore found widespread ...
Luisa B. Huber +4 more
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Dye structure affects Taq DNA polymerase terminator selectivity [PDF]
Nucleic Acids Research, 1999 All DNA sequencing methods have benefited from the development of new F667Y versions of Taq DNA polymerase. However, terminator chemistry methods show less uniform peak height patterns when compared to primer chemistry profiles suggesting that the dyes and/or their linker arms affect enzyme selectivity.
John W. Brandis
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One-Enzyme Reverse Transcription qPCR Using Taq DNA Polymerase
Biochemistry, 2020 Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine leukemia virus reverse transcriptase and has in the past been shown to possess reverse ...
Sanchita Bhadra +3 more
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A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor [PDF]
Scientific ReportsThe DNA polymerase derived from Thermus aquaticus is the most widely utilized among various DNA polymerases, indicating its significant economic importance. Consequently, efforts to achieve a substantial yield of Taq DNA polymerase (Taq-pol) are ongoing.
Fina Amreta Laksmi +11 more
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Investigation of the stability of D5SIC-DNAM-incorporated DNA duplex in Taq polymerase binary system: a systematic classical MD approach. [PDF]
Phys Chem Chem PhysDebnath T, Cisneros GA.
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dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase. [PDF]
Nucleic Acids Res, 2018 Zasedateleva OA +9 more
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Post-PCR Labeling Using Taq DNA Polymerase
BioTechniques, 1997 Zhidong Chen, Duane E. Ruffner
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Single-molecule Taq DNA polymerase dynamics [PDF]
Science Advances, 2022 Taq DNA polymerase functions at elevated temperatures with fast conformational dynamics—regimes previously inaccessible to mechanistic, single-molecule studies. Here, single-walled carbon nanotube transistors recorded the motions of Taq molecules processing matched or mismatched template–deoxynucleotide triphosphate pairs from 22° to 85°C.
Mackenzie W. Turvey +12 more
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