Results 31 to 40 of about 166,603 (275)
Liang you shipin ke-ji, 2023 Taq DNA polymerase is a thermostable enzyme and widely used in PCR technology. In order to improve the production of Taq DNA polymerase by recombinant Escherichia coli, in this study the enzyme yield and specific activity of the enzyme were taken as the
XIAO Yun, CHEN Jie
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The Indian Journal of Agricultural Sciences, 2022 PCR amplification and detection of GC rich sequences in DNA is a challenge due to formation of secondary structures which resist denaturation, thereby stalling Taq DNA polymerases as well as affecting primer annealing.
GAURAV DHAWAN +6 more
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Production and evaluation of egg derived hot start antibodies
Electronic Journal of Biotechnology, 2020 Background: Hot start can greatly improve specificity, sensitivity and yield of PCR. Non-specific amplification can occur in PCR when reaction mixture is prepared at room temperature, because Taq DNA polymerase is active and the primers can hybridize non-
Vladyslav Spyrydonov +4 more
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Genomic footprinting with Taq polymerase [PDF]
Nature, 1989 The linear amplification of chemically sequenced DNA with DNA polymerase from Thermus aquaticus permits the direct detection of DNA methylation and protein-DNA interaction sites in vivo.
H P, Saluz, J P, Jost
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Taq DNA polymerase blockage at pyrimidine dimers [PDF]
Nucleic Acids Research, 1996 Nucleic Acids Research, 24 (8)
Wellinger, Ralf-Erik, Thoma, Fritz
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Biotechnology Reports, 2018 Using Sso7d from Sulfolobus solfataricus as the DNA binding protein fused to Taq DNA polymerase at its amino terminus, we report the hyper-expression and a novel purification methodology of Sso7d-Taq polymerase (S-Taq) using aqueous two-phase extraction ...
Sudarson Sundarrajan +4 more
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Instant Taq: Rapid Autoinducible Expression and Chromatography-free Purification of Taq polymerase [PDF]
, 2021 AbstractDNA modifying enzymes are ubiquitous reagents in synthetic biology. Producing these enzymes often requires large culture volumes, purified nucleases and chromatographic separations to make enzymes of necessary quality. We sought to leverage synthetic biology tools to develop engineered strains allowing for not only the production but rapid ...
Romel Menacho-Melgar +2 more
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Data of expression and purification of recombinant Taq DNA polymerase
Data in Brief, 2016 Polymerase chain reaction (PCR) technique is widely used in many experimental conditions, and Taq DNA polymerase is critical in PCR process. In this article, the Taq DNA polymerase expression plasmid is reconstructed and the protein product is obtained ...
Na Fang +4 more
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Frontiers in Bioengineering and Biotechnology, 2021 A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes +3 more
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Brazilian Archives of Biology and Technology, 2011 We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects ...
Mohammadreza Hajjari, Behnaz Saffar
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