Liang you shipin ke-ji, 2023 Taq DNA polymerase is a thermostable enzyme and widely used in PCR technology. In order to improve the production of Taq DNA polymerase by recombinant Escherichia coli, in this study the enzyme yield and specific activity of the enzyme were taken as the
XIAO Yun, CHEN Jie
doaj +1 more source
Obstacles of Multiplex Real-Time PCR for Bacterial 16S rDNA: Primer Specifity and DNA Decontamination ofTaqPolymerase [PDF]
Transfusion Medicine and Hemotherapy, 2010 Sebastian Philipp +3 more
openalex +2 more sources
KLF6 and TP53 mutations are a rare event in prostate cancer: distinguishing between Taq polymerase artifacts and true mutations [PDF]
Modern Pathology, 2008 Laia Agell +8 more
openalex +2 more sources
The Indian Journal of Agricultural Sciences, 2022 PCR amplification and detection of GC rich sequences in DNA is a challenge due to formation of secondary structures which resist denaturation, thereby stalling Taq DNA polymerases as well as affecting primer annealing.
GAURAV DHAWAN +6 more
doaj +1 more source
Genomic footprinting with Taq polymerase [PDF]
Nature, 1989 The linear amplification of chemically sequenced DNA with DNA polymerase from Thermus aquaticus permits the direct detection of DNA methylation and protein-DNA interaction sites in vivo.
H. P. Saluz, J. P. Jost
openaire +3 more sources
Switching the activity of Taq polymerase using clamp-like triplex aptamer structure
Nucleic Acids Research, 2020 In nature, allostery is the principal approach for regulating cellular processes and pathways. Inspired by nature, structure-switching aptamer-based nanodevices are widely used in artificial biotechnologies.
Yingxin Hu +3 more
semanticscholar +1 more source
Taq DNA polymerase blockage at pyrimidine dimers [PDF]
Nucleic Acids Research, 1996 Nucleic Acids Research, 24 (8)
Wellinger, Ralf-Erik, Thoma, Fritz
openaire +5 more sources
Identification of novel clostridium perfringens type E strains that carry an iota toxin plasmid with a functional enterotoxin gene [PDF]
, 2011 Clostridium perfringens enterotoxin (CPE) is a major virulence factor for human gastrointestinal diseases, such as food poisoning and antibiotic associated diarrhea. The CPE-encoding gene (cpe) can be chromosomal or plasmid-borne.
A Deguchi +32 more
core +8 more sources
Rapid amplification of the RM-Yplex assay [PDF]
, 2016 A multiplex PCR assay consisting of 13 Rapidly Mutating Y STR loci called RM-Yplex was previously developed. Platinum® Taq DNA polymerase was used to amplify the 13 Y STR loci in a single reaction at an amplification time of approximately 2.5 h. In order
Abuidrees, A, Alghafri, A, Hadi, S
core +1 more source
P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer. [PDF]
, 1996 Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens ...
Chen, KM +5 more
core +2 more sources