Results 31 to 40 of about 819,635 (326)

Optimization of Taq DNA polymerase enzyme expression in Escherichia coli

Advanced Biomedical Research, 2012
In the present study, we optimized the experimental conditions using pET15b expression vector to obtain large amounts of Taq DNA polymerase.Correct framing of the gene in the expression vector pET15b and its orientation were analyzed by digestion and sequencing.
Mehran Miroliaei, Ali Rastegari, Fateme Moazen, Heydar Sadeghi, Sayed Mehdi Hoseini, Mojtaba Panjehpour   +5 more
openaire   +5 more sources

Comparison between Taq DNA Polymerase and Its Stoffel Fragment for Quantitative Real-Time PCR with Hybridization Probes [PDF]

BioTechniques, 2001
In quantitative real-time PCR assays, fluorophor-labeled oligonucleotide probes are employed to generate sequence-specific signals for the quantitative evaluation. Whereas TaqMan® probes have to be hydrolyzed during PCR by the endonucleolytic activity of
Jochen Wilhelm, Alfred Pingoud, Meinhard Hahn   +2 more
doaj   +2 more sources

A comparative study of extraction free detection of HBV DNA using sodium dodecyl sulfate, N-lauroylsarcosine sodium salt, and sodium dodecyl benzene sulfonate [PDF]

Scientific Reports
This study aimed to develop an extraction-free method for quantitative and qualitative detection of HBV DNA compared to traditional nucleic acid extraction.
Ko Ko, Lyubov Mikhailovna Lokteva, Golda Ataa Akuffo, Zayar Phyo, Chanroth Chhoung, E. Bunthen, Serge Ouoba, Aya Sugiyama, Tomoyuki Akita, Kim Rattana, Ork Vichit, Kazuaki Takahashi, Junko Tanaka   +12 more
doaj   +2 more sources

A-Tailing with Taq Polymerase v1

, 2015
This protocol can be used to add As to the blunt-ends of DNA fragments that have been amplified using a high-fidelity polymerase (such as Q5® High Fidelity DNA Polymerase).
N. Biolabs
openaire   +3 more sources

Replication of cyclobutane pyrimidine dimer analogue by Ex Taq DNA polymerase

Science and Technology of Advanced Materials, 2007
We previously reported an efficient and reversible template-directed photoligation using 5-carboxyvinyl-2'-deoxyuridine (CVU)-containing ODN at the 5'-terminal.
Masayuki Ogino et al
doaj   +2 more sources

Production of Reverse Transcriptase and DNA Polymerase in Bacterial Expression Systems [PDF]

Bioengineering
DNA amplification and reverse transcription enzymes have proven to be invaluable in fast and reliable diagnostics and research applications because of their processivity, specificity, and robustness.
Kristína Hriňová, Johana Dlapová, Bohuš Kubala, Ľubica Kormanová, Zdenko Levarski, Eva Struhárňanská, Ján Turňa, Stanislav Stuchlík   +7 more
doaj   +2 more sources

Amplimers with 3'-terminal phosphorothioate linkages resist degradation by vent polymerase and reduce Taq polymerase mispriming.

PCR methods and applications, 1992
The 3'-->5' exonuclease activity of Vent, a thermostable polymerase from Thermococcus litoralis, enhances DNA replication fidelity but also diverts PCR primers (amplimers) from targeted amplification by degrading their 3' termini.
C M de Noronha, James I. Mullins
openalex   +2 more sources

Increased efficiency of the Taq polymerase catalyzed polymerase chain reaction [PDF]

Nucleic Acids Research, 1989
Stephen A. Krawetz, Gordon H. Dixon, Richard T. Pon   +2 more
openaire   +5 more sources

Taq polymerase contains bacterial DNA of unknown origin

Molecular and Cellular Probes, 1990
The polymerase chain reaction (PCR) was carried out with the highly conserved E. coli ribosomal RNA gene sequences 1376-1395 and 1521-1540. Using these primers and reaction conditions specified by the manufacturer(s), a 165 bp fragment was synthesized using Taq polymerase from three different sources in the absence of any added template.
Department of Pathology University of Florida Gainesville, Florida, U.S.A. ( host institution ), Rand, Kenneth H. ( author ), Houck, Herbert ( author )   +2 more
openaire   +5 more sources

Taq-Polymerase [PDF]

Springer Reference Medizin, 2018
J. Arnemann
openaire   +3 more sources