Results 41 to 50 of about 819,635 (326)
Post-PCR Labeling Using Taq DNA Polymerase
BioTechniques, 1997 Duane E. Ruffner, Zhidong Chen
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Liang you shipin ke-ji, 2023 Taq DNA polymerase is a thermostable enzyme and widely used in PCR technology. In order to improve the production of Taq DNA polymerase by recombinant Escherichia coli, in this study the enzyme yield and specific activity of the enzyme were taken as the
XIAO Yun, CHEN Jie
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KLF6 and TP53 mutations are a rare event in prostate cancer: distinguishing between Taq polymerase artifacts and true mutations [PDF]
Modern Pathology, 2008 Laia Agell +8 more
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The Indian Journal of Agricultural Sciences, 2022 PCR amplification and detection of GC rich sequences in DNA is a challenge due to formation of secondary structures which resist denaturation, thereby stalling Taq DNA polymerases as well as affecting primer annealing.
GAURAV DHAWAN +6 more
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DNA sequencing using Taq polymerase
Nucleic Acids Research, 1988 Three DNA polymerases, namely E.coli DNA polymerase 1 (Klenow), reverse transcriptase and T7 DNA polymerase (sequenase), are commonly used for DNA sequencing by the chain termination method of Sanger and colleagues [1).
M. Peterson
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Frequent contamination of Taq polymerase with DNA
Clinical Chemistry, 1990 E. Böttger
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Analytical Chemistry, 2019 The incorporation of AuNPs into polymerase chain reaction (PCR) has become a promising strategy to develop sensitive sensing platforms, due to desirable optical properties of AuNPs and the exponential amplification power of PCR.
Jing Wang +4 more
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One enzyme reverse transcription qPCR using Taq DNA polymerase
bioRxiv, 2020 Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine
S. Bhadra +3 more
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Frontiers in Microbiology, 2014 DNA polymerases are widely used for DNA manipulation in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. Thermostable DNA polymerases are especially useful and became quite valuable after the development of PCR ...
Takeshi eYamagami +4 more
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Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]
, 2009 The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek +28 more
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