Results 41 to 50 of about 666,195 (194)

Polymerase Chain Reaction-Dynamic Light Scattering Sensor for DNA and Protein by Using Both Replication and Cleavage Properties of Taq Polymerase.

Analytical Chemistry, 2019
The incorporation of AuNPs into polymerase chain reaction (PCR) has become a promising strategy to develop sensitive sensing platforms, due to desirable optical properties of AuNPs and the exponential amplification power of PCR.
Jing Wang, Tingting Li, Ruidi Shen, Gongke Li, L. Ling   +4 more
semanticscholar   +1 more source

One enzyme reverse transcription qPCR using Taq DNA polymerase

bioRxiv, 2020
Taq DNA polymerase, one of the first thermostable DNA polymerases to be discovered, has been typecast as a DNA-dependent DNA polymerase commonly employed for PCR. However, Taq polymerase belongs to the same DNA polymerase superfamily as the Molony murine
S. Bhadra, A. C. Maranhao, Inyup Paik, A. Ellington   +3 more
semanticscholar   +1 more source

Mutant Taq DNA polymerases with improved elongation ability as a useful reagent for genetic engineering

Frontiers in Microbiology, 2014
DNA polymerases are widely used for DNA manipulation in vitro, including DNA cloning, sequencing, DNA labeling, mutagenesis, and other experiments. Thermostable DNA polymerases are especially useful and became quite valuable after the development of PCR ...
Takeshi eYamagami, Sonoko eIshino, Yutaka eKawarabayasi, Yutaka eKawarabayasi, Yoshizumi eIshino   +4 more
doaj   +1 more source

Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses [PDF]

, 2017
G-quadruplexes (G4s) are secondary structures of nucleic acids that epigenetically regulate cellular processes. In the human immunodeficiency lentivirus 1 (HIV-1), dynamic G4s are located in the unique viral LTR promoter.
A Ayouba, A Henderson, A Rambaut, AK Todd, AM Fleming, AN Lane, AR Haeusler, B Berkhout, B Zhou, BF Keele, D Piekna-Przybylska, D Qu, D Rhodes, D Sen, DJ Patel, E Tosoni, EA Raiber, F Barre-Sinoussi, F Benachenhou, F Bibollet-Ruche, F Heuverswyn Van, F Turrini, G Biffi, GE Crooks, IT Holder, J Felsenstein, J Norseen, J Qiu, J Song, J Yamaguchi, JE Clements, JF Fisette, JP Taylor, K Tamura, K Tluckova, KA Jones, LA Pereira, M Fry, M Metifiot, M Sala, M Scalabrin, MA Larkin, N Maizels, ND Perkins, NH Campbell, P Fratta, P Ivanov, P Murat, P Sket, R Perrone, R Perrone, R Perrone, R Perrone, RC Edgar, RC Gallo, RJ Gifford, S Amrane, S Artusi, S Artusi, S Balasubramanian, S Bhatia, S Broos, S Burge, S Pohlmann, SE Tong-Starksen, SR Wang, SR Wang, Y Wang   +67 more
core   +1 more source

Preparation of Taq DNA Polymerase [PDF]

BIO-PROTOCOL, 2011
1. Dialysis tubing cellulose membrane avg. flat width 33 mm (Sigma-Aldrich, catalog number: D9652) 2. E.coli BL21 strain 3. Ammonium sulfate (Sigma-Aldrich, catalog number: A4418) 4. IPTG 5. Ampicillin 6. (NH4)2SO4 7. 1 M dextrose 8. 1 M Tris HCl (pH 7.9) 9. 0.5 M EDTA (pH 8.0) 10. KCl 11. PMSF 12. Tween 20 13. NP40 14. Lysozyme 15. Glycerol 16. Triton-
openaire   +1 more source

The construction of a short gene by a very fast, modified, and simplified gene synthesis and the analysis of various effects on this synthesis

Brazilian Archives of Biology and Technology, 2011
We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects ...
Mohammadreza Hajjari, Behnaz Saffar
doaj   +1 more source

In situ PCR for Mycobacterium tuberculosis in endoscopic mucosal biopsy specimens of intestinal tuberculosis and Crohn disease [PDF]

, 2008
Tuberculosis and Crohn disease are granulomatous disorders affecting the intestinal tract with similar clinical manifestations and pathologic features.
Donoghue, HD, Peter, S, Pulimood, AB, Rook, GWA   +3 more
core   +1 more source

T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase

BMC Research Notes, 2018
Objectives In a recent publication, we reported the successful use of tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) for genotyping of rs445709131-SNP responsible for the bovine leukocyte adhesion ...
Rafeeque R. Alyethodi, Umesh Singh, Sushil Kumar, Rani Alex, Rajib Deb, Gyanendra S. Sengar, T. V. Raja, B. Prakash   +7 more
doaj   +1 more source

Chimerization of antibodies by isolation of rearranged genomic variable regions by the polymerase chain reaction [PDF]

, 1991
We describe a new method for amplification, by polymerase chain reaction (PCR), of rearranged segments encoding the variable part of light and heavy chains of an antibody (Ab) from the chromosomal DNA of hybridoma cells for the chimerization ofAbs.
Kaluzab, Brigitte, Schwirzke, Marina, Weidle, Ulrich H., Weiss, Elisabeth H., Weissenhorn, Winfried   +4 more
core   +1 more source

Influence of salts and pcr inhibitors on the amplification capacity of three thermostable DNA polymerases

Macedonian Journal of Chemistry and Chemical Engineering, 2010
The full potential of PCR as a rapid DNA detection method, is limited by inhibition of thermostable DNA polymerases by components in biological samples and substances used for purification of template DNA.
Katarina Davalieva, Georgi D. Efremov
doaj   +1 more source