Kinetic analysis of an efficient DNA-dependent TNA polymerase. [PDF]
, 2005 alpha-l-Threofuranosyl nucleoside triphosphates (tNTPs) are tetrafuranose nucleoside derivatives and potential progenitors of present-day beta-d-2'-deoxyribofuranosyl nucleoside triphosphates (dNTPs).
Chaput, John C +6 more
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Structure and possible function of a G-quadruplex in the long terminal repeat of the proviral HIV-1 genome [PDF]
, 2016 open8The long terminal repeat (LTR) of the proviral human immunodeficiency virus (HIV)-1 genome is integral to virus transcription and host cell infection.
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Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
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Distinguishing low frequency mutations from RT-PCR and sequence errors in viral deep sequencing data [PDF]
, 2015 There is a high prevalence of coronary artery disease (CAD) in patients with left bundle branch block (LBBB); however there are many other causes for this electrocardiographic abnormality.
Haydon, Daniel T. +6 more
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An efficient method for DNA extraction from Cladosporioid fungi [PDF]
, 2010 We developed an efficient method for DNA extraction from Cladosporioid fungi, which are important fungal plant pathogens. The cell wall of Cladosporioid fungi is often melanized, which makes it difficult to extract DNA from their cells.
Abd-Elsalam, K.A. +3 more
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Production and evaluation of egg derived hot start antibodies
Electronic Journal of Biotechnology, 2020 Background: Hot start can greatly improve specificity, sensitivity and yield of PCR. Non-specific amplification can occur in PCR when reaction mixture is prepared at room temperature, because Taq DNA polymerase is active and the primers can hybridize non-
Vladyslav Spyrydonov +4 more
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Evaluation of Different Amplification Protocols for Use in Primer-Extension Preamplification
BioTechniques, 1996 Different amplification protocols were evaluated for use with primer-extension preamplification (PEP). We hypothesized that a protocol known to improve amplification of long DNA fragments would improve efficacy of PEP. Eight DNA samples were preamplified
Eduardo Casas, Brian W. Kirkpatrick
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A Genomic Point Mutation in the Extracellular Domain of the Thyrotropin Receptor in Patients with Graves’ Ophthalmopathy [PDF]
, 1994 Orbital and pretibial fibroblasts are targets of autoimmune attack in Graves' ophthalmopathy (GO) and pretibial dermopathy (PTD). The fibroblast autoantigen involved in these peripheral manifestations of Graves' disease and the reason for the association
Bahn, Rebecca S. +3 more
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Heat-Mediated Activation of Affinity-Immobilized Taq DNA Polymerase
BioTechniques, 1997 A novel strategy for heat-mediated activation of recombinant Taq DNA polymerase is described. A serum albumin binding protein tag is used to affinity-immobilize an E. coli-expressed Taq DNA polymerase fusion protein onto a solid support coated with human
J. Nilsson +5 more
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Replicative bypass studies of l-deoxyribonucleosides in Vitro and in E. coli cell
Scientific Reports, 2022 l-nucleosides were the most important antiviral lead compounds because they can inhibit viral DNA polymerase and DNA synthesis of many viruses, whereas they may lead to mutations in DNA replication and cause genomic instability.
Yuhe Kan +6 more
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