Results 51 to 60 of about 819,635 (326)
Brazilian Archives of Biology and Technology, 2011 We have constructed a short gene with a single step assembly PCR without any additional amplification primers with Taq and Pfu polymerases. Since the Taq polymerase is a common and conventional enzyme for PCR reactions, we have analyzed various effects ...
Mohammadreza Hajjari, Behnaz Saffar
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P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer. [PDF]
, 1996 Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens ...
Chen, KM +5 more
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T-ARMS PCR genotyping of SNP rs445709131 using thermostable strand displacement polymerase
BMC Research Notes, 2018 Objectives In a recent publication, we reported the successful use of tetra primer-amplification refractory mutation system based polymerase chain reaction (T-ARMS-PCR) for genotyping of rs445709131-SNP responsible for the bovine leukocyte adhesion ...
Rafeeque R. Alyethodi +7 more
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Animal Research and One Health, EarlyView., 2023 In our study, we conducted circRNA expression profiling of Zhongwei goat skin exhibiting divergent curvature wool phenotypes at two developmental stages using the RNA‐seq. Functional verification assay demonstrated that circRNA8782 regulated fibroblast proliferation.
Yingxiao Su +9 more
wiley +1 more source
Macedonian Journal of Chemistry and Chemical Engineering, 2010 The full potential of PCR as a rapid DNA detection method, is limited by inhibition of thermostable DNA polymerases by components in biological samples and substances used for purification of template DNA.
Katarina Davalieva, Georgi D. Efremov
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Brazilian Journal of Medical and Biological Research, 1998 For certain applications of the polymerase chain reaction (PCR), it may be necessary to consider the accuracy of replication. The breakthrough that made PCR user friendly was the commercialization of Thermus aquaticus (Taq) DNA polymerase, an enzyme that
R.S. Diaz, E.C. Sabino
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Production and evaluation of egg derived hot start antibodies
Electronic Journal of Biotechnology, 2020 Background: Hot start can greatly improve specificity, sensitivity and yield of PCR. Non-specific amplification can occur in PCR when reaction mixture is prepared at room temperature, because Taq DNA polymerase is active and the primers can hybridize non-
Vladyslav Spyrydonov +4 more
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Road blocks on paleogenomes - polymerase extension profiling reveals the frequency of blocking lesions in ancient DNA [PDF]
, 2010 Although the last few years have seen great progress in DNA sequence retrieval from fossil specimens, some of the characteristics of ancient DNA remain poorly understood. This is particularly true for blocking lesions, i.e.
Briggs, A. +5 more
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Evaluation of Different Amplification Protocols for Use in Primer-Extension Preamplification
BioTechniques, 1996 Different amplification protocols were evaluated for use with primer-extension preamplification (PEP). We hypothesized that a protocol known to improve amplification of long DNA fragments would improve efficacy of PEP. Eight DNA samples were preamplified
Eduardo Casas, Brian W. Kirkpatrick
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Replicative bypass studies of l-deoxyribonucleosides in Vitro and in E. coli cell
Scientific Reports, 2022 l-nucleosides were the most important antiviral lead compounds because they can inhibit viral DNA polymerase and DNA synthesis of many viruses, whereas they may lead to mutations in DNA replication and cause genomic instability.
Yuhe Kan +6 more
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