Results 51 to 60 of about 166,603 (275)

Replicative bypass studies of l-deoxyribonucleosides in Vitro and in E. coli cell

Scientific Reports, 2022
l-nucleosides were the most important antiviral lead compounds because they can inhibit viral DNA polymerase and DNA synthesis of many viruses, whereas they may lead to mutations in DNA replication and cause genomic instability.
Yuhe Kan, Zhaoyang Jin, Yongqi Ke, Dao Lin, Liang Yan, Li Wu, Yujian He   +6 more
doaj   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Potential role of vitamin D receptor-related polymorphisms in bronchopulmonary dysplasia

Egyptian Journal of Medical Human Genetics, 2021
Background The potential contribution of vitamin D and its receptor (VDR) to bronchopulmonary dysplasia (BPD) in preterm neonates is still unknown. The objective of the study was to test the relationship between VDR Taq 1 and Fok 1 gene polymorphisms and
Walaa Alsharany Abuelhamd, Nancy Abdel Salam Gomaa, Alaa Gad, Rehab El-Wakeel   +3 more
doaj   +1 more source

Analysis of Treacher Collins syndrome 4‐associated mutations in Schizosaccharomyces pombe

FEBS Open Bio, EarlyView.
Fission yeast models carrying Treacher Collins syndrome type 4‐associated mutations reveal that impaired processivity of RNA polymerase I leads to defective rRNA transcription. This study highlights the essential role of a conserved arginine residue in Pol I elongation and provides mechanistic insight into the pathogenesis of ribosomopathies.
Kei Kawakami, Hiroaki Kato
wiley   +1 more source

A strong strand displacement activity of thermostable DNA polymerase markedly improves the results of DNA amplification

BioTechniques, 2014
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov, Ekaterina V. Barsova, Arkady F. Fradkov, Konstantin A. Blagodatskikh, Tatiana V. Kramarova, Vladimir M. Kramarov   +5 more
doaj   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

FEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava, Anoop Saraya, Deepak Gunjan, Rinu Sharma   +3 more
wiley   +1 more source

A Fusion of Taq DNA Polymerase with the CL7 Protein from Escherichia coli Remarkably Improves DNA Amplification

Molecules
DNA polymerases are important enzymes that synthesize DNA molecules and therefore are critical to various scientific fields as essential components of in vitro DNA synthesis reactions, including PCR.
Zhongchen Li, Yaping Wang, Xiangyi Wang, Shuhui Niu, Zhenlong Su, Fei Wang, Jing Ni, Yan Gong, Ben Rao   +8 more
doaj   +1 more source

OTIMIZAÇÃO DA TÉCNICA DA PCR PARA A DETECÇÃO DO POLIMORFISMO CYP3A4*1B E FREQUÊNCIA ALÉLICA ENTRE 60 MORADORES DE SÃO LUÍS/MA

Cadernos de Pesquisa, 2013
Os objetivos deste trabalho foram adequar a técnica de PCR para o polimorfismo CYP3A4*1B, reduzir as amplificações inespecíficas observadas e verificar a frequência alélica do polimorfismo na população de São Luís/MA. A reação de PCR foi padronizada para
Bruna Aparecida Penha, Israel Higino de Sousa, Wandson Rodrigues Sousa, Jomar Diogo Nunes, Marcelo dos Santos, Sally Cristina Moutinho Monteiro   +5 more
doaj   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Fusion of Taq DNA polymerase with single-stranded DNA binding-like protein of Nanoarchaeum equitans-Expression and characterization.

PLoS ONE, 2017
DNA polymerases are present in all organisms and are important enzymes that synthesise DNA molecules. They are used in various fields of science, predominantly as essential components for in vitro DNA syntheses, known as PCR.
Marcin Olszewski, Marta Śpibida, Maciej Bilek, Beata Krawczyk   +3 more
doaj   +1 more source