Results 61 to 70 of about 819,635 (326)
Heat-Mediated Activation of Affinity-Immobilized Taq DNA Polymerase
BioTechniques, 1997 A novel strategy for heat-mediated activation of recombinant Taq DNA polymerase is described. A serum albumin binding protein tag is used to affinity-immobilize an E. coli-expressed Taq DNA polymerase fusion protein onto a solid support coated with human
J. Nilsson +5 more
doaj +1 more source
Potential role of vitamin D receptor-related polymorphisms in bronchopulmonary dysplasia
Egyptian Journal of Medical Human Genetics, 2021 Background The potential contribution of vitamin D and its receptor (VDR) to bronchopulmonary dysplasia (BPD) in preterm neonates is still unknown. The objective of the study was to test the relationship between VDR Taq 1 and Fok 1 gene polymorphisms and
Walaa Alsharany Abuelhamd +3 more
doaj +1 more source
A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement [PDF]
, 2013 A significantly improved DNA polymerase fidelity assay, based on a gapped plasmid containing the lacZα reporter gene in a single-stranded region, is described.
Connolly, Bernard A +2 more
core +1 more source
Advanced Biology, EarlyView.Hypoxia promotes the epithelial‐mesenchymal transition (EMT) of renal tubular epithelial cells via the SIRT1‐FoxO1‐FoxO3‐autophagy pathway, thereby resulting in the fibrosis of renal tubular epithelial cells. Activation of SIRT1 or induction of autophagy inhibits this process, alleviating hypoxia‐induced fibrosis.
Guangyu Wang +6 more
wiley +1 more source
CLONING AND CHARACTERIZATION OF ISOLATED TAQ DNA POLYMERASE GENE FROM PHAGE [PDF]
, 2009 Taq polymerase is a thermo stable DNA polymerase named after the thermophilic bacterium T.aquaticus, having a molecular weight of 66,000-94,000 Daltons. Taq DNA polymerases have become a valuable tool in today’s research. The choice of the DNA polymerase
KUMAR, ANIL, MISHRA, NIKHIL
core +1 more source
Advanced Photonics Research, EarlyView.This study demonstrates a label‐free optical method to monitor cells using a terahertz metasurface‐based biosensing platform. The obtained terahertz signal change for the cell‐contained nanoslots before and after external stimulus is analyzed in terms of transmittance and frequency shift, dynamically representing the cell apoptosis process.
Yeeun Roh +4 more
wiley +1 more source
BioTechniques, 2014 The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov +5 more
doaj +1 more source
Modification Of Taq DNA Polymerase with Improved Elongation Ability
Eurasian Journal of Applied Biotechnology, 2021 Thermostable polymerases play a significant role in molecular biology and diagnostic practice. The most famous and demanded is Polymerase I from the thermophilic bacterium Thermus aquaticus (Taq-pol). This polymerase at one time made a kind of revolution in the polymerase chain reaction. In this work, we attempted to modify this polymerase by attaching
M. Saginova +4 more
openaire +3 more sources
Inhibition of DNA polymerase reactions by pyrimidine nucleotide analogues lacking the 2-keto group [PDF]
, 2017 To investigate the influence of the pyrimidine 2-keto group on selection of nucleotides for incorporation into DNA by polymerases, we have prepared two C nucleoside triphosphates that are analogues of dCTP and dTTP, namely 2-amino-5-(2′-deoxy-β-d ...
Guo, Mao-Jun +5 more
core
Nucleic Acid Lateral Flow Immunoassay for the Detection of Pathogenic Bacteria from Food [PDF]
, 2009 Nucleic acid lateral flow immunoassay (NALFIA) is a method combining molecular biological principle of detection with immunochemical principle of visualisation.
Amerongen, A., van +5 more
core +2 more sources