Potential role of vitamin D receptor-related polymorphisms in bronchopulmonary dysplasia
Background The potential contribution of vitamin D and its receptor (VDR) to bronchopulmonary dysplasia (BPD) in preterm neonates is still unknown. The objective of the study was to test the relationship between VDR Taq 1 and Fok 1 gene polymorphisms and
Walaa Alsharany Abuelhamd+3 more
doaj +1 more source
De novo Development and Characterization of Tetranucleotide Microsatellite Loci Markers from a Southeastern Population of the House Finch (Haemorhous mexicanus) [PDF]
Microsatellites are short tandem repeats (e.g. TAGATAGA) of base pairs in a species’ genome. High mutation rates in these regions produce variation in the number of repeats across individuals that can be utilized to study patterns of population- and ...
Barron, Douglas G+2 more
core +3 more sources
AmpliTaq DNA Polymerase, FS Dye-Terminator Sequencing: Analysis of Peak Height Patterns
Taq DNA polymerases in which the phenylalanine is substituted by a tyrosine at position 667 (Taq F667Y) are members of a new class of DNA polymerases that incorporate chain-terminating dideoxyribonucleoside triphosphates (ddNTPs) much more efficiently ...
L.T. Parker+5 more
doaj +1 more source
Transduction of DNA information through water and electromagnetic waves
The experimental conditions by which electromagnetic signals (EMS) of low frequency can be emitted by diluted aqueous solutions of some bacterial and viral DNAs are described.
Aïssa, Jamal+9 more
core +1 more source
Single genome sequencing of near full-length HIV-1 RNA using a limiting dilution approach [PDF]
Sequencing very long stretches of the HIV-1 genome can advance studies on virus evolution and in vivo recombination but remains technically challenging.
Demecheleer, Els+7 more
core +1 more source
DNA polymerases are important enzymes that synthesize DNA molecules and therefore are critical to various scientific fields as essential components of in vitro DNA synthesis reactions, including PCR.
Zhongchen Li+8 more
doaj +1 more source
The sensitivity and robustness of various DNA detection and amplification techniques are to a large extent determined by the properties of the DNA polymerase used.
Konstantin B. Ignatov+5 more
doaj +1 more source
Modification Of Taq DNA Polymerase with Improved Elongation Ability
Thermostable polymerases play a significant role in molecular biology and diagnostic practice. The most famous and demanded is Polymerase I from the thermophilic bacterium Thermus aquaticus (Taq-pol). This polymerase at one time made a kind of revolution in the polymerase chain reaction. In this work, we attempted to modify this polymerase by attaching
M. Saginova+4 more
openaire +3 more sources
Impact of metal oxide nanoparticles on in vitro DNA amplification [PDF]
Polymerase chain reaction (PCR) is used as an in vitro model system of DNA replication to assess the genotoxicity of nanoparticles (NPs). Prior results showed that several types of NPs inhibited PCR efficiency and increased amplicon error frequency.
Chun-Hui Gao+8 more
doaj +2 more sources
Optimizing Illumina next-generation sequencing library preparation for extremely AT-biased genomes. [PDF]
BAckground: Massively parallel sequencing technology is revolutionizing approaches to genomic and genetic research. Since its advent, the scale and efficiency of Next-Generation Sequencing (NGS) has rapidly improved. In spite of this success, sequencing
Campino, Susana+10 more
core