Results 71 to 80 of about 666,195 (194)
Identification of Five Putative Yeast RNA Helicase Genes [PDF]
, 1990 The RNA helicase gene family encodes a group of eight homologous proteins that share regions of sequence similarity. This group of evolutionarily conserved proteins presumably all utilize ATP (or some other nucleoside triphosphate) as an energy source ...
Abelson, John +2 more
core +1 more source
A potent inhibitor of Taq polymerase copurifies with human genomic DNA.
Nucleic Acids Research, 1988 The polymerase chain reaction (PCR) is a rapid method for amplifying a defined sequence from small amounts of input DNA (1). We have encountered a potent inhibitor of Taq polymerase in some samples of genomic DNA extracted from human blood (2).
R. Franchis +3 more
semanticscholar +1 more source
Cadernos de Pesquisa, 2013 Os objetivos deste trabalho foram adequar a técnica de PCR para o polimorfismo CYP3A4*1B, reduzir as amplificações inespecíficas observadas e verificar a frequência alélica do polimorfismo na população de São Luís/MA. A reação de PCR foi padronizada para
Bruna Aparecida Penha +5 more
doaj +1 more source
Fidelity of DNA polymerases in the detection of intraindividual variation of mitochondrial DNA
Mitochondrial DNA. Part B. Resources, 2020 Here we investigated the consequences of PCR amplification errors in the identification of intraindividual mtDNA variation. The bumblebee Bombus morio was chosen as model for the COI gene amplification tests with two DNA polymerases (Taq and Q5 ...
Paulo Cseri Ricardo +2 more
doaj +1 more source
Efficient DNA isolation from moroccan arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the rapd-pcr molecular technique [PDF]
, 2010 Efficient DNA isolation from Moroccan Arar tree [Tetraclinis articulata (Vahl) Masters] leaves and optimization of the RAPD-PCR molecular technique. Molecular genetic analysis of Arar tree [Tetraclinis articulata (Vahl) Masters] is often limited by the
Abourouh, Mohamed +4 more
core +2 more sources
Nucleic Acids Research, 1989 Polyacrylamide gel electrophoresis of DNA fragments obtained by the polymerase chain reaction using Taq polymerase revealed the presence of multiple fragments shorter than the expected product.
D. Olsen, F. Eckstein
semanticscholar +1 more source
Biotechnology Reports, 2018 Using Sso7d from Sulfolobus solfataricus as the DNA binding protein fused to Taq DNA polymerase at its amino terminus, we report the hyper-expression and a novel purification methodology of Sso7d-Taq polymerase (S-Taq) using aqueous two-phase extraction ...
Sudarson Sundarrajan +4 more
doaj
The reproducibility of RAPD profiles: Effects of PCR components on RAPD analysis of four centaurium species [PDF]
Archives of Biological Sciences, 2012 Random amplified polymorphic DNA (RAPD) analysis is a simple and reliable method used to detect DNA polymorphism. Several factors can affect the amplification profiles, thereby causing false bands and non-reproducibility of the assay.
Skorić Marijana +6 more
doaj +1 more source
Effect of Agitation on Taq DNA Polymerase Production by Escherichia Coli in Bioreactor
Chemical Engineering Transactions, 2022 The Taq DNA polymerase is an important protein used during PCR (Polymerase chain reaction), a technique widely used today. The production of this enzyme in bioreactors make possible to improve efficiency, to reduce production time, to increase production
Diana Morales-Fonseca +5 more
doaj +1 more source
Polymerase-endonuclease amplification reaction for large-scale enzymatic production of antisense oligonucleotide [PDF]
, 2009 Synthetic oligonucleotides are contaminated with highly homologous failure sequences. Oligonucleotide synthesis is difficult to scale up because it requires expensive equipments, hazardous chemicals, and tedious purification process.
Deming Gou, Xiaolong Wang
core +1 more source