Results 71 to 80 of about 166,603 (275)

Epstein‐Barr Virus Expressed Long Non‐Coding RNA (lncBARTs) Regulate EBV Latent Genome Replication

Advanced Science, EarlyView.
EBV produces abundant level of lncBARTs, which are essential for maintaining viral genome replication in EBV‐associated cancers. LncBARTs interact with a complex comprising BRD4, CTCF and viral protein EBNA1 at EBV oriP region. This interaction tethers oriP to host chromosomes, facilitating EBV episome replication.
Jiayan Liu, Dittman Lai‐Shun Chung, Larry Ka‐Yue Chow, Shuo Han, Wenkai Yi, Conor J. Cremin, Yingyin Liao, Pui Wang, Bobo Wing‐Yee Mok, Mingfang Ji, Jian Yan, Wei Dai, Honglin Chen   +12 more
wiley   +1 more source

Gut Microbiota‐Derived Anandamide Mediates the Therapeutic Effects of Urolithin A on Alcohol‐Induced Cognitive and Social Dysfunction via CB1R‐DRD2‐RAP1 Signaling Axis

Advanced Science, EarlyView.
This study reveals that Urolithin A (UA) counteracts alcohol‐induced cognitive and social dysfunction (AICSD) via a gut microbiome‐dependent mechanism. UA‐enriched Bacteroids sartorii and Parabacteroids distasonis elevate anandamide (AEA), which activates the CB1R‐DRD2‐Rap1 signaling cascade to drive synaptic repair and reduce neuroinflammation ...
Hongbo Zhang, Zibin Li, Yao Xiao, Ji Bian, Caian He, Chao Liu, Lan Gong, Lin Han, Zhigang Liu, Min Wang   +9 more
wiley   +1 more source

Data of self-made Taq DNA polymerase prepared for screening purposes

Data in Brief, 2017
DNA analysis is a key procedure in genetic engineering. Nowadays the analysis is often done by PCR with Taq DNA polymerase. Although the last enzyme price is quite low, demand for numerous analyses results in much money expenditure which are not ...
E.V. Konovalova, A.A. Schulga, T.I. Lukyanova, E.J. Woo, S.M. Deyev   +4 more
doaj   +1 more source

Vitamin D receptor polymorphisms among the Turkish population are associated with multiple sclerosis

Balkan Journal of Medical Genetics, 2023
Multiple sclerosis (MS) is an inflammatory disease characterized by demyelination and axonal degeneration affecting the central nervous system. Among the genetic factors suggested to be associated with this disease are polymorphisms to the vitamin D ...
Bulan B, Hoscan AY, Keskin SN, Cavus A, Culcu EA, Isik N, List EO, Arman A   +7 more
doaj   +1 more source

Inactivation of AXL in Cardiac Fibroblasts Alleviates Right Ventricular Remodeling in Pulmonary Hypertension

Advanced Science, EarlyView.
Pulmonary hypertension (PH) is a progressive condition with high morbidity and mortality, largely owing to right ventricular (RV) failure resulting from maladaptive remodeling. Our study provides strong evidence in support of a critical, detrimental role for AXL as a previously unrecognized determinant driving RV fibrotic pathology in PH.
Li‐Wei Wu, Min Chen, Chen‐Yu Jiang, Dai‐Ji Jiang, Xu Zhang, Xiao‐He Xu, Yi‐Wei Liu, Bei Feng, Lin‐Cai Ye, Yang‐Yang He, Xu Huang, Yi‐Chi Zhang, Xing‐Liang Zhou, Yi Shen, Tian‐Yu Liu, Li‐Jun Fu, Yi Yan, Hao Zhang   +17 more
wiley   +1 more source

Optimization of inter simple sequence repeats (ISSR) analysis as applied to preliminary study of genetic variation in Abies beshanzuensis M. H. Wu

浙江大学学报. 农业与生命科学版, 2005
Factors which affect the ISSR analysis of Abies beshanzuensis, such as annealing temperature and concentrations of template DNA, Taq DNA polymerase, Mg2+ and dNTP, etc. were examined. The optimal ISSR-PCR reaction system (25 μL) in our experiments was as
AI Jian-guo, QIU Ying-xiong, YU Jiu-hua, CHEN Xiao-rong, DING Bing-yang   +4 more
doaj   +1 more source

Preparation of Taq DNA Polymerase

BIO-PROTOCOL, 2011
1. Dialysis tubing cellulose membrane avg. flat width 33 mm (Sigma-Aldrich, catalog number: D9652) 2. E.coli BL21 strain 3. Ammonium sulfate (Sigma-Aldrich, catalog number: A4418) 4. IPTG 5. Ampicillin 6. (NH4)2SO4 7. 1 M dextrose 8. 1 M Tris HCl (pH 7.9) 9. 0.5 M EDTA (pH 8.0) 10. KCl 11. PMSF 12. Tween 20 13. NP40 14. Lysozyme 15. Glycerol 16. Triton-
openaire   +1 more source

Efisiensi Penggunaan Enzim Taq Polymerase pada Pengujian Polymerase Chain Reaction

Buletin Veteriner Udayana, 2022
Polymerase Chain Reaction (PCR) merupakan uji diagnostik yang sangat sensitif. Enzim Taq Polymerase merupakan salah satu komponen paling penting dalam pengujian Polymerase Chain Reaction (PCR). Enzim taq polymerase merupakan komponen yang harganya paling mahal. Penggunaan taq polymerase pada uji PCR kurang efisien. Untuk mengetahui efisiensi penggunaan
I Gusti Ngurah Kade Mahardika, Ni Putu Sutrisna Dewi, Ni Made Ritha Krisna Dewi, Luh Dewi Anggreni   +3 more
openaire   +1 more source

Alkyltriphenylphosphonium Binding to Cardiolipin Triggers Oncosis in Cancer Cells

Advanced Science, EarlyView.
Alkyltriphenylphosphonium, exemplified by TPP+‐C14, preferentially accumulates in mitochondria and selectively binds to cardiolipin, a key phospholipid of the inner mitochondrial membrane, causing loss of mitochondrial membrane potential, severe cellular ATP depletion, and calcium imbalance.
Jin Li, Hang Zheng, Yuxing Lin, Ziwei Zhao, Yijia Mai, Zhangrong Lou, Qiang Liu, Zhao Ma, Chengjun Wu   +8 more
wiley   +1 more source

Notch2 Directs aTreg Cell Fate toward Immunoregulation or Inflammatory Pyroptosis

Advanced Science, EarlyView.
Schematic illustration showing that the Notch2 intracellular domain (NICD2) facilitates pyroptosis resistance in activated Tregs through the RREB1/Foxo1 signaling pathway. In addition, Notch2‐mediated pyroptosis resistance in activated Tregs promotes immunoregulatory capacity, thereby attenuating Th2‐driven inflammatory responses in allergic rhinitis ...
Yue‐Long Qiao, Shan Xu, You Zou, Rui Yang, Han Zheng, Jia‐Yu Liu, Hui‐Ming Fan, Yuan‐Yuan Zhang, Yong‐Gang Kong, Wo‐Er Jiao, Shi‐Ming Chen   +10 more
wiley   +1 more source