Results 81 to 90 of about 819,635 (326)

KLF1 Promotes Cardiomyocyte Proliferation and Heart Regeneration Through Regulation of Wnt/β‐Catenin Signaling Pathway

Advanced Science, EarlyView.
In KLF1‐on (neonatal and Klf1 overexpression hearts) models, KLF1 regulates Wnt/β‐catenin signaling pathway and the expression of cell proliferation‐related genes via transcriptional and epigenetic mechanisms, which induces cardiomyocyte proliferation and cardiac regeneration in mice after myocardial infarction.
Yanglin Hao, Xi Zhang, Shuan Ran, Yuan Li, Weicong Ye, Song Wang, Xiaohan Li, Zilong Luo, Jiulu Zhao, Junjie Zong, Kexiao Zheng, Ran Li, Han Zhang, Longyong Lai, Pinyan Huang, Zifeng Zou, Wang Zhan, Zhang Yue, Jie Wu, Jiahong Xia   +19 more
wiley   +1 more source

Incomplete primer extension during in vitro DNA amplification catalyzed by Taq polymerase; exploitation for DNA sequencing.

Nucleic Acids Research, 1989
Polyacrylamide gel electrophoresis of DNA fragments obtained by the polymerase chain reaction using Taq polymerase revealed the presence of multiple fragments shorter than the expected product.
D. Olsen, F. Eckstein
semanticscholar   +1 more source

Mapping and characterization of G-quadruplexes in Mycobacterium tuberculosis gene promoter regions [PDF]

, 2017
Mycobacterium tuberculosis is the causative agent of tuberculosis (TB), one of the top 10 causes of death worldwide in 2015. The recent emergence of strains resistant to all current drugs urges the development of compounds with new mechanisms of action ...
Lavezzo, Enrico, Manganelli, Riccardo, Pal\ue3\ub9, Giorgio, Perrone, Rosalba, Provvedi, Roberta, Richter, Sara N., Riello, Erika, Toppo, Stefano   +7 more
core   +1 more source

Novel properties of recombinant Sso7d-Taq DNA polymerase purified using aqueous two-phase extraction: Utilities of the enzyme in viral diagnosis

Biotechnology Reports, 2018
Using Sso7d from Sulfolobus solfataricus as the DNA binding protein fused to Taq DNA polymerase at its amino terminus, we report the hyper-expression and a novel purification methodology of Sso7d-Taq polymerase (S-Taq) using aqueous two-phase extraction ...
Sudarson Sundarrajan, Sreesada Parambath, Swetha Suresh, Sneha Rao, Sriram Padmanabhan   +4 more
doaj  

Activation of HTR2B Suppresses Osteosarcoma Progression through the STAT1‐NLRP3 Inflammasome Pathway and Promotes OASL1+ Macrophage Production to Enhance Antitumor Immunity

Advanced Science, EarlyView.
Activation of HTR2B suppresses osteosarcoma progression through the STAT1‐NLRP3 inflammasome pathway and promotes OASL1+ Macrophage production to enhance anti‐tumor immunity. Abstract Osteosarcoma is a primary malignant bone tumor originating from mesenchymal tissue, and associated with poor prognosis.
Zhen Huang, Jiazhuang Zhu, Jianping Hu, Xingkai Wang, Xiaolong Ma, Enjie Xu, Kunpeng Zhu, Chunlin Zhang   +7 more
wiley   +1 more source

Optimization of degenerate oligonucleotide primed PCR for amplification of microdissected chromosome segments [PDF]

, 1994
The degenerate oligonucleotide primer of sequence, 5\u27 CCGACTCGAGNNNNNNATGTGG 3\u27, contains a specific 3\u27 hexanucleotide , a central random hexanucleotide and an Xhol site towards it\u27s 5\u27 end.
Mahjoubi, Frouzandeh
core   +1 more source

Label-free optical detection of single enzyme-reactant reactions and associated conformational changes

, 2017
Monitoring the kinetics and conformational dynamics of single enzymes is crucial in order to better understand their biological functions as these motions and structural dynamics are usually unsynchronized among the molecules.
Baaske, Martin D., Kim, Eugene, Schuldes, Isabel, Vollmer, Frank, Wilsch, Peter S.   +4 more
core   +1 more source

Conserved presence of G-quadruplex forming sequences in the Long Terminal Repeat Promoter of Lentiviruses [PDF]

, 2017
G-quadruplexes (G4s) are secondary structures of nucleic acids that epigenetically regulate cellular processes. In the human immunodeficiency lentivirus 1 (HIV-1), dynamic G4s are located in the unique viral LTR promoter.
A Ayouba, A Henderson, A Rambaut, AK Todd, AM Fleming, AN Lane, AR Haeusler, B Berkhout, B Zhou, BF Keele, D Piekna-Przybylska, D Qu, D Rhodes, D Sen, DJ Patel, E Tosoni, EA Raiber, F Barre-Sinoussi, F Benachenhou, F Bibollet-Ruche, F Heuverswyn Van, F Turrini, G Biffi, GE Crooks, IT Holder, J Felsenstein, J Norseen, J Qiu, J Song, J Yamaguchi, JE Clements, JF Fisette, JP Taylor, K Tamura, K Tluckova, KA Jones, LA Pereira, M Fry, M Metifiot, M Sala, M Scalabrin, MA Larkin, N Maizels, ND Perkins, NH Campbell, P Fratta, P Ivanov, P Murat, P Sket, R Perrone, R Perrone, R Perrone, R Perrone, RC Edgar, RC Gallo, RJ Gifford, S Amrane, S Artusi, S Artusi, S Balasubramanian, S Bhatia, S Broos, S Burge, S Pohlmann, SE Tong-Starksen, SR Wang, SR Wang, Y Wang   +67 more
core   +1 more source

Vitamin D receptor polymorphisms among the Turkish population are associated with multiple sclerosis

Balkan Journal of Medical Genetics, 2023
Multiple sclerosis (MS) is an inflammatory disease characterized by demyelination and axonal degeneration affecting the central nervous system. Among the genetic factors suggested to be associated with this disease are polymorphisms to the vitamin D ...
Bulan B, Hoscan AY, Keskin SN, Cavus A, Culcu EA, Isik N, List EO, Arman A   +7 more
doaj   +1 more source

Enhanced Activities of OCT4 and SOX2 Promote Epigenetic Reprogramming by Shortening G1 Phase

Advanced Science, EarlyView.
Fusing the VP16 domain to OCT4 and SOX2 (OvSvK) enhances iPSC generation by activating downstream targets, including those regulating the cell cycle. This accelerates reprogramming by shortening the G1 phase and reducing H3K27me3 levels. Modulating Ccnd1, Cdkn2a, and Ccne1 improves efficiency, linking cell cycle to epigenetic remodeling.
Lin Guo, Jiechun Lin, Qiwen Ren, Hao Sun, Yanhua Wu, Haofei Ge, Xiaolan Wu, Lihui Lin, Lining Liang, Changpeng Li, He Liu, Yuangbang Mai, Shilong Chu, Jiadong Liu, Jing Liu, Jiekai Chen, Duanqing Pei, Hui Zheng   +17 more
wiley   +1 more source