Results 81 to 90 of about 666,195 (194)
Differentiation between Aspergillus flavus and Aspergillus parasiticus from Pure Culture and Aflatoxin-Contaminated Grapes Using PCR-RFLP Analysis of aflR-aflJ Intergenic Spacer [PDF]
, 2011 Aflatoxins (AFs) represent the most important single mycotoxin-related food safety problem in developed and developing countries as they have adverse effects on human and animal health. They are produced mainly by Aspergillus flavus and A.
Atoui, Ali +5 more
core +1 more source
Genomic homogeneity in fibrolamellar carcinomas [PDF]
, 2001 Background-Fibrolamellar carcinoma (FLC) is a variant of hepatocellular carcinoma (HCC) with distinctive clinical and histological features. To date there have been few studies on the genotypic aspects of FLC and no previous attempts have been made to ...
Davidson, BR +4 more
core +1 more source
Engineered DNA Polymerase Improves PCR Results for Plastid DNA
Applications in Plant Sciences, 2013 Premise of the study: Secondary metabolites often inhibit PCR and sequencing reactions in extractions from plant material, especially from silica-dried and herbarium material.
Melanie Schori +3 more
doaj +1 more source
Vitamin D receptor polymorphisms among the Turkish population are associated with multiple sclerosis
Balkan Journal of Medical Genetics, 2023 Multiple sclerosis (MS) is an inflammatory disease characterized by demyelination and axonal degeneration affecting the central nervous system. Among the genetic factors suggested to be associated with this disease are polymorphisms to the vitamin D ...
Bulan B +7 more
doaj +1 more source
Inhibition of DNA polymerase reactions by pyrimidine nucleotide analogues lacking the 2-keto group [PDF]
, 2017 To investigate the influence of the pyrimidine 2-keto group on selection of nucleotides for incorporation into DNA by polymerases, we have prepared two C nucleoside triphosphates that are analogues of dCTP and dTTP, namely 2-amino-5-(2′-deoxy-β-d ...
Guo, Mao-Jun +5 more
core
大腸菌を用いたフォスファカン（コンドロイチン硫酸プロテオグリカン）の融合コア蛋白の発現条件の検討 [PDF]
, 1996 Optimal conditions for expressing a specific region of core protein of phosphacan, a chondroitin sulfate proteoglycan known as receptor type protein tyrosine phosphatase, as fusion protein with glutathione S-transferase (GST) in E.coli were examined. DNA
Ito, Sekiko, Okamoto, Motoi
core +1 more source
Thermodynamic and Structural Origins for the Extreme Stability of Taq DNA Polymerase [PDF]
Biophysical Journal, 2009 The thermal stability of Taq DNA polymerase is well known, and is the basis for its use in PCR. We have previously shown that at 25°C, Klentaq polymerase (the large fragment domain of Taq) has a folding free energy (ΔGfold) of −27 kcal/mole, making it one of the most stable monomeric proteins yet characterized.
Vince J. LiCata +2 more
openaire +2 more sources
مجله دانشکده پزشکی اصفهان, 2012 Background: The aim of this project was to isolate and purify the highly active recombinant Taq DNA polymerase from the strain of Escherichia coli BL21.
Hamid Mirmohammad Sadeghi +3 more
doaj
PRISE2: software for designing sequence-selective PCR primers and probes. [PDF]
, 2014 BackgroundPRISE2 is a new software tool for designing sequence-selective PCR primers and probes. To achieve high level of selectivity, PRISE2 allows the user to specify a collection of target sequences that the primers are supposed to amplify, as well as
Borneman, James +3 more
core +2 more sources
浙江大学学报. 农业与生命科学版, 2005 Factors which affect the ISSR analysis of Abies beshanzuensis, such as annealing temperature and concentrations of template DNA, Taq DNA polymerase, Mg2+ and dNTP, etc. were examined. The optimal ISSR-PCR reaction system (25 μL) in our experiments was as
AI Jian-guo +4 more
doaj +1 more source