Results 81 to 90 of about 496,503 (216)
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Global costs of attaining the Millennium Development Goal for water supply and sanitation
Bulletin of the World Health Organization, 2008 OBJECTIVE: Target 10 of the Millennium Development Goals (MDGs) is to "halve by 2015 the proportion of people without sustainable access to safe drinking water and basic sanitation".
Guy Hutton, Jamie Bartram
doaj
A Residual Optronic Convolutional Neural Network for SAR Target Recognition
PhotonicsDeep learning (DL) has shown great capability in remote sensing and automatic target recognition (ATR). However, huge computational costs and power consumption are challenging the development of current DL methods.
Ziyu Gu +4 more
doaj +1 more source
FEBS Open Bio, EarlyView.The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Drugs previously repurposed to target blood cancers reduced neuroblastoma and glioblastoma cell growth and viability. However, their levels of anticancer activity were different and their clinical application may be problematic due to side effects at effective doses.
Abhishek Kharawatkar +4 more
wiley +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Directed evolution of enzymes at the crossroads of tradition and innovation
FEBS Open Bio, EarlyView.An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova +2 more
wiley +1 more source
Clientelism, coalitions, and concessions: pragmatism in local elections and its democratic costs
Frontiers in Political ScienceIntroductionThis study examines how clientelism, coalitions, and political concessions shape democratic costs in local elections, with political pragmatism as a mediating factor.MethodsWe conducted an anonymous survey of 100 voters in Southeast Sulawesi,
Anwar Sadat, Muh Askal Basir
doaj +1 more source
How phagocytic cells kill bacteria: Lessons from a professional killer
FEBS Open Bio, EarlyView.How phagocytic cells ingest and kill bacteria has been studied for more than a century, but many questions remain unanswered. The study of the amoeba Dictyostelium discoideum brings new answers, and new questions. Professional phagocytic cells such as neutrophils and macrophages, as well as free‐living soil amoebae like Dictyostelium discoideum, employ
Otmane Lamrabet, Pierre Cosson
wiley +1 more source
EnergiesTo achieve sustainable development, the transition from a fossil-based economy to a circular economy is essential. The use of renewable energy sources to make the overall carbon foot print more favorable is an important pre-requisite. In this context, it
Aristide Giuliano +3 more
doaj +1 more source