Results 61 to 70 of about 534,578 (304)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

Testing the Bounds: Empirical Behavior of Target Zone Fundamentals [PDF]

open access: yes
Standard target zone exchange rate models are based on nonlinear functions of an unobserved economic fundamental, which is assumed to be bounded, similarly to the target zone exchange rates themselves.
J. Isaac Miller
core  

TUITION/PRICING FOR ONLINE LEARNING

open access: yesOnline Learning, 2019
To make online learning an integral part of higher education, institutions must determine the real costs of instruction and what tuition to charge based on these costs. Then the question is: Is this tuition bearable by the target population of potential
Karen Paulson
doaj   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Target Costing: um perfil das publicações entre os anos de 2000 a 2005 [PDF]

open access: yes, 2006
TCC (Graduação) - Universidade Federal de Santa Catarina. Centro Socioeconômico. Curso de Ciências ContábeisEste trabalho visa traçar um perfil das publicações sobre Target Costing divulgadas nos principais periódicos, de Contabilidade e Administração ...
Shimabukuro, Karina Mitiko
core  

Hemoglobin stability impact on healthcare resource utilization and costs among dialysis-dependent patients with anemia of end-stage kidney disease

open access: yesBMC Nephrology
Background The impact of hemoglobin stability on healthcare resource utilization (HCRU) and costs in dialysis-dependent patients with anemia of end-stage kidney disease (ESKD) was evaluated. Methods This retrospective, observational study used Optum’s de-
Anna D. Coutinho   +6 more
doaj   +1 more source

COSTS AND BENEFITS OF THE PARIS CLIMATE TARGETS

open access: yesClimate Change Economics, 2023
The temperature targets in the Paris Agreement cannot be met without very rapid reduction of greenhouse gas emissions and removal of carbon dioxide from the atmosphere. The latter requires large, perhaps prohibitively large subsidies. The central estimate of the costs of climate policy, unrealistically assuming least-cost implementation, is 3.8–5.6 ...
openaire   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Lean Accounting: Measuring Target Costs [PDF]

open access: yes, 2012
Aerospace is very important to the Canadian economy, with over 80,000 employees; generating over $20 billion dollars in revenue. However, the industry is facing many challenges. With the economic downturn, sales have been decreasing.
Salam, Adil
core  

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