Results 131 to 140 of about 229,816 (269)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Suprathreshold non-volatile flavour perception is associated with multiple species rather than any single species via bacterial metabolism. [PDF]

J Oral Microbiol
Zhang S, Shoaie S, Carpenter GH.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

EEG Cross-Subject Taste Classification Method: A Meta-Learning Wavelet Graph Convolutional Neural Network Under Sweet and Bitter Stimuli. [PDF]

Biosensors (Basel)
Wang H, Men H, Shi Y.
europepmc   +1 more source

Dual native G‐quadruplex folding is associated with chromatin looping at the MYC locus

FEBS Open Bio, EarlyView.
BG4‐detectable G‐quadruplex (G4) in HaCaT and NHEK keratinocytes identified folded and unfolded G4s enriched at promoters/TSSs and active enhancers, whereas unfolded G4s also overlapped weak/poised enhancers. At MYC–PVT1, 3C‐qPCR detected enhancer–promoter looping only when G4s were simultaneously folded at both regulatory elements under native ...
Dieila Giomo de Lima, Gustavo Narvaes Guimarães, Emilyane de Oliveira Santana Amaral, Bianca Caroline Figueiredo Bianco, Manuel Jara‐Espejo, Sérgio Roberto Peres Line, Ana Paula de Souza, Aline Cristiane Planello   +7 more
wiley   +1 more source

Prevalence and characteristics of persistent taste and smell dysfunction after immune checkpoint inhibitor therapy for cancer. [PDF]

Support Care Cancer
van Elst JM, Buffinga CM, Brand HS, Hijmering-Kappelle LBM, Jager-Wittenaar H, Reyners AKL, Nuver J, de Haan JJ.   +7 more
europepmc   +1 more source

Risk Prediction Models for Recurrence After Curative Treatment of Early‐Stage or Locally Advanced Lung Cancer: A Systematic Review

Aging and Cancer, EarlyView.
This systematic review synthesizes prognostic models for survival and recurrence in resected non‐small cell lung cancer. While many models demonstrate moderate to good discrimination, few are externally validated and reporting quality is variable, limiting clinical applicability and highlighting the need for robust, transparent model development ...
Evangeline Samuel, S. Tissera, E. Paul, J. Zalcberg, R. G. Stirling   +4 more
wiley   +1 more source

Comprehensive Taste Profile Assessment of Underexplored Amino Acids and Protein Derivatives in Umami and Koku. [PDF]

Foods
López Martínez MI, Hopf A, Salvador A, Toldrá F, Forde C, Mora L.   +5 more
europepmc   +1 more source

Five‐Year Outcomes of Laparoscopic Sphincter‐Preserving Surgery for Rectal Cancer: A Prospective Study in Elderly Vietnamese Patients

Aging and Cancer, EarlyView.
This prospective study demonstrates that laparoscopic sphincter‐preserving surgery is feasible for elderly patients. While overall survival reaches 70% at 5 years, advanced T‐stage and the omission of neoadjuvant therapy significantly drive recurrence, highlighting the need for personalized geriatric protocols despite logistical challenges.
Huu Duc Ho, Quang Le, Ngoc Thach Tran, Van Viet Ung, Loc Vu   +4 more
wiley   +1 more source

Effects of Non-Nutritive Sweeteners and Sweet Taste Exposure on Weight Management, Biomarkers of Health and Sweet Taste Preference-A Review of the Evidence from Recent European Consortia Studies. [PDF]

Nutrients
Čad EM, Appleton KM, Blaak EE, Dakin C, de Graaf K, Finlayson G, Forde CG, Halford JCG, Kjølbæk L, Mars M, Martinez JA, Navas-Carretero S, Raben A, Scott C, Harrold JA.   +14 more
europepmc   +1 more source