Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Increasing Accuracy and Realism of Simulated Naval Tactics with Monterey Phoenix Behavior Models
NPS NRP Executive SummaryLive training environments that develop Marine combat skills deliver real physical conditions and evoke the realistic physical and mental stresses of anticipated operational environments.
Giammarco, Kristin
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Chromones as Photocatalyzed HAT Reagent and Michael Acceptor for Direct C-H Alkylation of 2-Substituted 4-Chromanones. [PDF]
Research (Wash D C)Xiao Y +6 more
europepmc +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Implementing R&D policies : an analsysis of Spain´s pharmaceutical research program. [PDF]
We analyze Spain’s National Pharmaceutical Research Program using detailed firm-l vel data. We fin differences between ex ante announced evaluation criteria and ex post implementation.
Lobo, Félix +2 more
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Interventions against loneliness and social isolation in older adults- a systematic review. [PDF]
BMC Public HealthBergsträsser J +3 more
europepmc +1 more source
Why human connection is the true metric of research success
FEBS Open Bio, EarlyView.Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan +3 more
wiley +1 more source
The Impact of the Joint Interagency Field Experimentation Program on Small Business Success
Excerpt from the Proceedings of the Twenty-Second Annual Acquisition Research Symposium and Innovation Summit.This paper examines the impact of the Joint Interagency Field Experimentation (JIFX) program at the Naval Postgraduate School (NPS) on the ...
Book, Ashley
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Economic Proximity and Technology Flows: South Africa?s Influence and the Role of Technological Interaction in Botswana?s Diversification Effort [PDF]
In less than a decade after the end of the apartheid, South Africa has intensified its economic ties with its African neighbours to become one of the top-ten investors and trading partners of many African countries, displacing companies from the former ...
Habi Yaremye, Alexis
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