Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Council on Technology Teacher Education Yearbook Indices Compendium
, 2009 This document covers Yearbooks of the American Council on Industrial Arts Teacher Education and Council on Technology Teacher Education, 1952 – 2009. The list is not meant to replicate the indices which have appeared at the end of most of the yearbooks ...
Council on Technology Teacher Education (U.S.)
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Switching Middle School Teachers onto STEM Using a Pedagogical Framework for Technology Integration: The Case for High Possibility Classrooms in Australia [PDF]
, 2017 Education in STEM (Science, Technology, Engineering and Mathematics) is a significant issue for governments and organizations across the world as concerns are expressed about students’ lack of progress in these areas. In Australia, middle school teachers’
Hunter, JL
core
Indigenous Wisdom, Capital, Technology and Education. [PDF]
N Z J Educ Stud, 2020 Ruth D.
europepmc +1 more source
Improving the management of type 2 diabetes through large-scale general practice: the role of a data-driven and technology-enabled education programme. [PDF]
BMJ Open Qual, 2021 Radwan TF +7 more
europepmc +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
FEBS Open Bio, EarlyView.A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
FEBS Open Bio, EarlyView.Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
A Framework for Augmented Intelligence in Allergy and Immunology Practice and Research-A Work Group Report of the AAAAI Health Informatics, Technology, and Education Committee. [PDF]
J Allergy Clin Immunol Pract, 2022 Khoury P +6 more
europepmc +1 more source
FEBS Open Bio, EarlyView.TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source