Results 81 to 90 of about 1,273,672 (267)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Multi-Physics Modeling in Curved Surface Laser Cladding: Impact of Scanning Trajectories and Cladding Parameters on Temperature Field and Coating Thickness

Crystals
In order to apply laser cladding technology to the complex surface processing of hot-working dies, this study developed a numerical model for curved surface laser cladding along various scanning trajectories under multi-physics coupling considering the ...
Chenyun Deng, Wei Chen, Yingxia Zhu
doaj   +1 more source

Hydrostatic pressure activates HIF‐1α via β‐catenin to promote stemness in breast cancer cells

FEBS Open Bio, EarlyView.
To mimic the elevated intestinal fluid pressure in breast cancers, we loaded human breast cancer cells (MCF‐7, MDA‐MB‐453, and BT‐474) to 50 mmHg hydrostatic pressure. Hydrostatic pressure exposure upregulated HIF‐1α and induced stemness in MCF‐7 and BT‐474 cells.
Da Zhai, Yong Xu, Jingjing Ma, Ratchanee Duangrat, Chen Yan, Jingyan Liang, Hua Wei, Bin Jiang, Tao‐Sheng Li   +8 more
wiley   +1 more source

Temperature Fields Induced by Low Power Focused Ultrasound in Soft Tissues During Gene Therapy. Numerical Predictions and Experimental Results

Archives of Acoustics, 2014
The aim of this work is twofold. Firstly, to verify a theoretical model which is capable of predicting temperature fields appearing in soft tissues during their ultrasound treatment.
Barbara GAMBIN, Tamara KUJAWSKA, Eleonora KRUGLENKO, Andrzej MIZERA, Andrzej NOWICKI   +4 more
doaj  

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Temperature Field Analysis and Simulation and Optimization of Flow Field in High-low Temperature Test Chamber

Zhileng xuebao
In this study, a 294 L high-low temperature test chamber with two power batteries in the test area is investigated. The temperature rise and heat dissipation of the batteries in the chamber at a constant temperature of 45 ℃ are simulated through CFD, and
Zheng Xiaoteng, Liu Jinping
doaj  

A Study on the Evaluation Method for the Operating Status of Overhead Transmission Lines Based on Analytic Hierarchy Process (AHP)

Engineering Reports
To address the challenge of dynamically assessing overhead transmission lines under varying meteorological conditions through manual inspections and drone monitoring, this study proposes an evaluation method for the operating status of overhead ...
Qingbin Wang, Liang Wang, Rui Tian, Shuangpeng Cao, Shuai Zuo, Di Che, Lantao Jing   +6 more
doaj   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Spatiotemporal Analysis of Ventilation Efficiency in Single-Span Plastic Greenhouses in Hot-Humid Regions of China: Using Validated CFD Modeling

Agriculture
To characterize the spatiotemporal distribution of temperature and airflow in single-span plastic-film greenhouses, we coupled field experiments with three-dimensional computational fluid dynamics (CFD) simulations in a warm–temperate region of China ...
Song Wang, Naimin Kong, Lirui Liang, Yuexuan He, Wenjun Peng, Xiaohan Lu, Chi Qin, Zijing Luo, Wei Zhao, Chengyao Jiang, Mengyao Li, Yangxia Zheng, Wei Lu   +12 more
doaj   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source