Results 101 to 110 of about 1,780,646 (296)

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Temperature Changes in Meat Products Fried in Steam-Convection Oven

Техника и технология пищевых производств
Combination steam ovens, or combi steamers, have entered all spheres of food production, including the meat industry. Their rational use requires a scientific and practical foundation.
Marina N. Smagina, Denis A. Smagin
doaj   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Experimental Study on Monitoring Equipment for the Scouring and Sedimentation of Wharf Bank Slopes Based on Heat Transfer Principles

Sensors
The scouring and sedimentation of wharf bank slopes significantly impact port safety and efficiency. To overcome the limitations of existing monitoring technologies in real-time capability, adaptability, and precision, this study introduces an innovative
Jilong Yin, Huaqing Zhang, Mengmeng Liu, Qian Ma   +3 more
doaj   +1 more source

Agricultural scene understanding [PDF]

The author has identified the following significant results. The LACIE field measurement data were radiometrically calibrated. Calibration enabled valid comparisons of measurements from different dates, sensors, and/or locations.
Bauer, M. E., Baumgardner, M. F., Hoffer, R. M., Landgrebe, D. A., Silva, L.   +4 more
core   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

Experimental analysis of glass failure criteria under different thermal conditions

Frontiers in Thermal Engineering
The initiation of the first crack mainly determines the criteria for glass failure. However, the extended consequences of cracking, with the formation of multiple cracks merging, result in fallout conditions under varying thermal loads that become more ...
Raj Kumar Mishra, Pawan Kumar Sharma, Ravi Kumar   +2 more
doaj   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Can thermoregulatory response to heat stress be improved in lactating dairy cows? Insights from counter-current heat transfer systems impacting reproduction

The Journal of Reproduction and Development
Climate change has caused heat stress (HS) to become an increasingly severe problem for high-producing dairy herds. Although cooling systems allow milk production to remain nearly constant throughout the year, fertility decreases during summer ...
Fernando LÓPEZ-GATIUS
doaj   +1 more source

Temperature gradients in female reproductive tissues [PDF]

Reproductive BioMedicine Online, 2012
Deep body temperature in mammals is generally but incorrectly regarded as uniform. Alterations of temperature in oviducts and preovulatory Graafian follicles may play a vital role in gamete maturation, fertilization and early embryonic development. At a molecular level, the conformation of regulatory proteins is susceptible to changes in temperature ...
openaire   +2 more sources