Results 81 to 90 of about 13,706,361 (339)
Effect of substrate thermal resistance on space-domain microchannel [PDF]
, 2009 In recent years, Fluorescent Melting Curve Analysis (FMCA) has become an almost ubiquitous feature of commercial quantitative PCR (qPCR) thermal cyclers. Here a micro-fluidic device is presented capable of performing FMCA within a microchannel.
Dalton, Tara +2 more
core +2 more sources
Application of Denaturing Gradient Gel ElectrophoresisApplication of Denaturing Gradient Gel Electrophoresis(DGGE) Methods on Parent-Offspring Relationship of the Coral Pocillopora damicornis [PDF]
, 2008 DGGE (Denaturing Gradient Gel Electrophoresis) is the most powerful methods for mutation detection currentlyavailable. In DGGE, DNA fragments of the same length but with different sequences can be separated.
Permata W, Diah, Hirose, M, Hidaka , M
core +1 more source
Peripheral lysosomes recruit PLEKHG3 to focal adhesions and restrain protrusion dynamics
FEBS Letters, EarlyView.Proximity‐dependent labeling at the LAMTOR complex revealed the Rho GEF PLEKHG3 as a lysosome‐proximal protein directing the study toward the influence of lysosome positioning on actin dynamics and cell motility. We show that PLEKHG3 colocalizes with lysosomes at focal adhesion sites and observe that forced peripheral dispersion of lysosomes hinders ...
Rainer Ettelt +8 more
wiley +1 more source
Liver organoids: modelling complexity in homeostasis and disease
FEBS Letters, EarlyView.Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley +1 more source
, 2000 Cowper-Symonds and Johnson-Cook dynamic constitutive relations are used to study the influence of both strain rate effect and temperature variation on the material intrinsic length scale in strain gradient plasticity.
Zhao YP(赵亚溥), Yu TX(余同希)
core +2 more sources
Dynamics of an insulating Skyrmion under a temperature gradient. [PDF]
Physical Review Letters, 2013 We study the Skyrmion dynamics in thin films under a temperature gradient. Our numerical simulations show that both single and multiple Skyrmions in a crystal move towards the high temperature region, which is contrary to particle diffusion.
Ling-Yao Kong, J. Zang
semanticscholar +1 more source
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Micro structure and Lagrangian statistcs of the scalar field with a mean gradient in isotropic turbulence [PDF]
, 2003 This paper presents an analysis and numerical study of the relations between the small-scale velocity and scalar fields in fully developed isotropic turbulence with random forcing of the large scales and with an imposed constant mean scalar gradient ...
Nieuwstadt, FTM +2 more
core
Artificial molecular machines and motors—Design and control of nanoscale motion
FEBS Letters, EarlyView.Molecules are constantly moving because of thermal fluctuations, but random motion alone cannot be exploited to perform directional tasks. Artificial molecular machines use chemical, electrical, or light energy to bias this motion. Molecular shuttles, rotary motors, and supramolecular pumps illustrate how nanoscale movement can be controlled and ...
Leonardo Andreoni, Alberto Credi
wiley +1 more source