Results 41 to 50 of about 3,990,525 (247)

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Possible Schemes and Effects in Improving Temperature Uniformity in Indirect Cooling Wine Cabinet

Zhileng xuebao, 2017
The development of a high-quality wine cabinet requires a method to make the temperature distribution in the cabinet as uniform as possible. In this study, basic ideas for achieving a uniform temperature in a wine cabinet are presented, including a ...
Zeng Xianshun, Zhao Dan, Ding Guoliang, Ding Jianbo, Li Jing   +4 more
doaj  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Research and Optimization of Liquid Cooling Plate for High-heat-flux Components

Zhileng xuebao, 2021
Fluid cooling plates are widely used in the heat dissipation of high-density components, which imposes stricter requirements for the temperature uniformity of the plate surface.
Zhan Binfei, Xu Hongbo, Zhang Hainan, Leng Dongmei, Tian Changqing, Zhou Yuan   +5 more
doaj  

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Research on Temperature Change Law and Non-Uniform Distribution Characteristics of Electromagnetic Control Roll Based on Rotating Heat Flow

Machines
The uniform temperature distribution on the surface of the electromagnetic control roll (ECR) has a great impact on the quality of the strip; therefore, temperature control is essential.
Shuaishuai Zheng, Tingsong Yang, Tieheng Yuan, Wenquan Sun, Ankang Shen, Shuo Fan   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Development and analysis of techniques to improve air-cooling and temperature uniformity in battery packs [PDF]

, 2017
One of the challenges to the advancement of electric vehicles is the requirement of an effective thermal management system to maintain the temperature, and temperature uniformity of the battery pack and the cell within the operating limits. In this study,
Shahid, Seham
core   +2 more sources

Surface temperature uniformity of tubes subjected to pool boiling

, 2023
This dataset was used to investigate the surface temperature uniformity of developing laminar flow through horizontal tubes submerged in boiling water. The surface temperatures, fluid temperatures and mass flow rates were measured.
Marilize Everts (13188639), Rudaviro Munzara (8553969)   +1 more
core   +1 more source

Treatment with KCL‐286, a first‐in‐class retinoic acid receptor‐β (RARβ) agonist, ameliorates neuronal DNA damage and inflammation in a mouse model of Alzheimer's disease

FEBS Open Bio, EarlyView.
Repair of neuronal DNA damage in Alzheimer's disease by KCL‐286. (A) Amyloid‐β oligomers and plaques impair neuronal DNA repair pathways, leading to DNA double‐strand breaks and glial activation. (B) KCL‐286 activates RARβ/RXR signalling via retinoic acid response elements (RAREs), associated with increased BRCA1 expression, enhanced DNA repair and ...
Natasha Hill, Hassan Yousef Othman AlMuallim, Elouisa Maddock, Carl Hobbs, Earl Clarke, Maria B. Goncalves, Jonathan P. T. Corcoran   +6 more
wiley   +1 more source