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Orchid Micropropagation Using Temporary Immersion Systems: A Review
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air.Obdulia, Baltazar-Bernal +2 more
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Alstroemeria Micropropagation in a RITA® Temporary Immersion System
The use of temporary immersion systems (TIS) for plant micropropagation is an efficient technique for plant production, and we have applied it for the production of alstroemerias. This method involves the cultivation of explants such as rhizomes and axillary buds in a nutrient medium to stimulate shoot growth.Lilia Castro Pereira +4 more
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Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems
Shoots from European chestnut and hybrids of European and Asian chestnuts can be efficiently proliferated in liquid medium by temporary immersion (TIS) using RITA® and Plantform™ bioreactors. The main challenges of applying TIS to these species were the lack of growth and hyperhydricity; problem solutions included the manipulation of the hormone ...Nieves, Vidal +2 more
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Application of a temporary immersion system in mass propagation of Phalaenopsis
2005A temporary immersion system consisting of a series of five-litre twin glass vessels was successfully used for in vitro adventitious shoot multiplication and rooting of Phalaenopsis. Highest shoot multiplication rate of 25.4 was achieved after twelve weeks when eight immersions per day, of ten minutes each, were used. Fresh medium containing 0.5 mg l−1
Tino Hempfling, Walter Preil
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Conclusions and Perspectives on Plant Micropropagation in Temporary Immersion Systems
In vitro propagation protocols that include temporary immersion systems are available for the most economically important plant species. However, these have not been established yet for multiple species. Having protocols validated by the scientific community guarantees the success of the mass production of commercial propagules.Marco A, Ramírez-Mosqueda +1 more
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Use of Temporary Immersion Systems in the Establishment of Biofactories
Companies dedicated to the large-scale propagation of plant species are known as biofactories or agricultural biotechnology companies. Globally, there are a large number of biofactories (large-scale production) or plant tissue culture laboratories (small-scale production) in charge of supplying commercial propagules of plants of economic interest. Eachopenaire +2 more sources
THE TEMPORARY IMMERSION SYSTEM (T.I.S.) FOR THE IMPROVEMENT OF MICROPROPAGATION OF ORNAMENTAL PLANTS
Acta Horticulturae, 2005The clonal propagation through tissue culture techniques has limitations in the flower industry due to the requirementof highly skilled manual labour and to the limitation of automatia. The growth in liquid medium is an interesting alternative that might provide opportunities only if it will be possible to control or avoid the tissue hyperhydration ...
B. Ruffoni, M. Savona
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Types of Temporary Immersion Systems Used in Commercial Plant Micropropagation
Technological innovation in the design and manufacture of temporary immersion systems (TIS) has increased in the past decade. Innovations have involved the size, fitting, and replacement of components, as well as manufacturing materials. Air replacement by compressor has also been substituted by air replacement by preset tilting/rotation of culture ...Ivonne N, Bravo-Ruíz +4 more
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Efficiency-cost, micropropagation, bioreactors, temporary immersion systems
Agro ProductividadObjective: to estimate the production price of strawberry “mother” plant of the CP-Jacona variety in both TIS and TS, and to compare these prices with the price of the imported “mother” plant. Design/Methodology/Approach: micropropagation methods have been used as an effective means for the mass production of pathogen-free plants, in small spaces and ...
Martha Alejandra Gomez Cruz +4 more
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Mass propagation of tropical crops in temporary immersion systems
2005Temporary immersion systems (TIS) have been described for in vitro multiplication of a wide range of tropical crops. Laboratory protocols are available for shoot multiplication, somatic embryo and microtuber production. Seven species are now commercially propagated by this culture technique (Ananas comosus, Coffea arabica, Cymbopogon citratus, Musa sp.,
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