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Types of Temporary Immersion Systems Used in Commercial Plant Micropropagation
Technological innovation in the design and manufacture of temporary immersion systems (TIS) has increased in the past decade. Innovations have involved the size, fitting, and replacement of components, as well as manufacturing materials. Air replacement by compressor has also been substituted by air replacement by preset tilting/rotation of culture ...Ivonne N, Bravo-Ruíz +4 more
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Efficiency-cost, micropropagation, bioreactors, temporary immersion systems
Agro ProductividadObjective: to estimate the production price of strawberry “mother” plant of the CP-Jacona variety in both TIS and TS, and to compare these prices with the price of the imported “mother” plant. Design/Methodology/Approach: micropropagation methods have been used as an effective means for the mass production of pathogen-free plants, in small spaces and ...
Martha Alejandra Gomez Cruz +4 more
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Mass propagation of tropical crops in temporary immersion systems
2005Temporary immersion systems (TIS) have been described for in vitro multiplication of a wide range of tropical crops. Laboratory protocols are available for shoot multiplication, somatic embryo and microtuber production. Seven species are now commercially propagated by this culture technique (Ananas comosus, Coffea arabica, Cymbopogon citratus, Musa sp.,
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TEMPORARY IMMERSION SYSTEMS FOR EFFICIENT MASS PROPAGATION OF MEDICINAL AND AROMATIC PLANTS
Acta Horticulturae, 2015Plant production through in vitro propagation is nowadays a well-established industry. Micropropagation protocols are widely based on semi-solid nutrient media, despite their inherent limitations. Expensive gelling agents, large numbers of relatively small containers, limited nutrient diffusion, large culture areas, and extensive manual handling can ...
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Temporary immersion system for in vitro propagation via organogenesis of forest plant species
Trees, 2023Yudith García-Ramírez
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Development of an easy in vitro culture system by temporary immersion
1995L'immersion temporaire est une technique permettant de réduire de nombreux inconvénients de la culture in vitro en milieu liquide. Pour pouvoir l'appliquer facilement et économiquement un appareillage et un système d'automatisation simples ont été développés. Son emploi au cours des phases successives du microbouturage ou de l'embryogénèse somatique de
Teisson, Claude +5 more
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Optimising cocoa somatic embryogenesis by using temporary immersion system [PDF]
Des embryons somatiques de cacaoyer peuvent être obtenus à partir de staminodes, mais également à partir de fragments de cotylédons d'embryons somatiques primaires. Ce système de culture permet une phase d'amplification de la production d'embryons. Ces cultures sont réalisées en milieu solide où l'amplification reste modérée.
Alemanno, Laurence +2 more
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In vitro multiplication of Eucalyptus globulus by temporary immersion system
2017Con el objeto de establecer metodologías de masificación de Eucalyptus globulus se analizaron factores que afectan la multiplicación in vitro de esta especie mediante el sistema de inmersión temporal. Para ello se dispusieron cinco microtallos por frasco, conteniendo 250 mL de medio Murashige y Skoog. Se estudiaron la frecuencia de inmersión (6, 12, 24,
González, Ricardo +3 more
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