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Micropropagation of Mokara Orchid by Temporary Immersion System Technique
International Journal of Research and Innovation in Applied Science, 2022Based young leaves of Mokara Leuen Berger Gold were used as cultured materials. Callus was initiated and increase biomass on medium of semisolid and liquid cultures: MS medium supplemented with CW (30%), sucrose (30 g/l), 2.4D (1 mg/l). Callus was used as materials for initiation and biomass propagation on medium: (1) MS medium supplemented with NAA (1
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Temporary Immersion Systems in Plant Micropropagation
Temporary immersion systems (TIS) are technological tools that support plant micropropagation. Given their high efficiency in the in vitro propagation of shoots, a current goal is to update the protocols addressing micropropagation in semisolid culture systems to protocols involving TIS.Marco A, Ramírez-Mosqueda +1 more
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Plant Micropropagation and Temporary Immersion Systems
Temporary immersion systems (TIS) have been widely recognized as a promising technology for micropropagation of various plant species. The TIS provides a suitable environment for culture and allows intermittent contact of the explant with the culture medium at different immersion frequencies and aeration of the culture in each cycle.Hugo A, Méndez-Hernández +1 more
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A TEMPORARY IMMERSION SYSTEM FOR THE EFFECTIVE SHOOT REGENERATION OF HOP
Acta Horticulturae, 2009The largest bottleneck for the genetic transformation of hop is the inefficiency of existing regeneration protocols. Successful hop transformation therefore requires large numbers of explants, is time consuming and labor intensive. We developed a fast and efficient shoot regeneration procedure for the 'US Tettnanger' cultivar, starting from green ...
A. Schwekendiek, S.T. Hanson, M. Crain
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BioMINT: A Temporary Immersion System for Agave Micropropagation
Agaves are cultivated in Mexico as a source of industrial products such as fibers, nutritional supplements, and alcoholic beverages. Due to the demand for plant material, its long-life cycle, and the need to avoid predation on its natural populations, in vitro micropropagation represents a good option for agaves.Kelly M, Monja-Mio +4 more
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Evaluation of a new vessel system based on temporary immersion system for micropropagation
Scientia Horticulturae, 2014a b s t r a c t The aim of this study was to evaluate a new culture vessel Plantform bioreactor, based on the temporary immersion system principle for micropropagation of Digitalis lutea × purpurea, Echinacea purpurea and Rubus idaeus. The multiplication ratio and shoot quality in bioreactor were either similar to or better than these on agar medium ...
M. Welander +3 more
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Orchid Micropropagation Using Temporary Immersion Systems: A Review
Temporary immersion systems (TIS) have been used for orchid micropropagation. The main advantage of TIS use for micropropagation is that the explant is periodically immersed in nutrient media, and then, the nutrient solution is drained, which allows the explant tissue to stay in air.Obdulia, Baltazar-Bernal +2 more
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Alstroemeria Micropropagation in a RITA® Temporary Immersion System
The use of temporary immersion systems (TIS) for plant micropropagation is an efficient technique for plant production, and we have applied it for the production of alstroemerias. This method involves the cultivation of explants such as rhizomes and axillary buds in a nutrient medium to stimulate shoot growth.Lilia Castro Pereira +4 more
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Proliferation of Axillary Shoots of Chestnut in Temporary Immersion Systems
Shoots from European chestnut and hybrids of European and Asian chestnuts can be efficiently proliferated in liquid medium by temporary immersion (TIS) using RITA® and Plantform™ bioreactors. The main challenges of applying TIS to these species were the lack of growth and hyperhydricity; problem solutions included the manipulation of the hormone ...Nieves, Vidal +2 more
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Application of a temporary immersion system in mass propagation of Phalaenopsis
2005A temporary immersion system consisting of a series of five-litre twin glass vessels was successfully used for in vitro adventitious shoot multiplication and rooting of Phalaenopsis. Highest shoot multiplication rate of 25.4 was achieved after twelve weeks when eight immersions per day, of ten minutes each, were used. Fresh medium containing 0.5 mg l−1
Tino Hempfling, Walter Preil
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