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Terminal deoxynucleotidyl transferase: Properties and applications [PDF]

Engineering Microbiology
Terminal deoxynucleotidyl transferase (TdT), a unique DNA polymerase, can elongate DNA by adding deoxynucleotides to the 3′ terminal of a DNA chain in a template-independent manner. Owing to their remarkable DNA synthesis activity, TdTs have promoted the
Chengjie Zhang, Hizar Subthain, Fei Guo, Peng Fang, Shanmin Zheng, Mengzhe Shen, Xianger Jiang, Zhengquan Gao, Chunxiao Meng, Shengying Li, Lei Du   +10 more
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Label-Free miRNA-21 Analysis Based on Strand Displacement and Terminal Deoxynucleotidyl Transferase-Assisted Amplification Strategy [PDF]

Biosensors, 2022
MicroRNAs (miRNAs) are regarded as a rising star in the biomedical industry. By monitoring slight increases in miRNA-21 levels, the possibilities of multi-type malignancy can be evaluated more precisely and earlier.
Ying Yan, Han Zhao, Yukang Fang, Changbei Ma, Junxiang Chen   +4 more
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Visual Detection of Cucumber Green Mottle Mosaic Virus Based on Terminal Deoxynucleotidyl Transferase Coupled with DNAzymes Amplification [PDF]

Sensors, 2019
A simple, rapid, and sensitive visual detection method for observing cucumber green mottle mosaic virus was reported based on the template-independent polymerization activity of terminal deoxynucleotidyl transferase (TdT), coupled with the cascade ...
Ying Wang, Jing Liu, Hong Zhou
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Transformation of a low-grade follicular lymphoma into a composite lymphoma combining a high-grade B-cell lymphoma and a lymphoblastic neoplasm expressing Terminal deoxynucleotidyl Transferase: a case report [PDF]

Journal of Medical Case Reports, 2020
Background High-grade B-cell lymphoma with rearrangements of MYC and BCL2 and/or BCL6 is an aggressive mature B-cell neoplasm, whereas B-lymphoblastic lymphoma is immature cell proliferation, with a frequent positivity for terminal deoxynucleotidyl ...
Antonin Bouroumeau, Eleonore Kaphan, Clémentine Legrand, Tatiana Raskovalova, Gautier Szymanski, Claire Vettier, Christine Lefebvre, Marie-Christine Jacob, Anne McLeer, Michel Peuchmaur, Rémy Gressin, Hervé Sartelet   +11 more
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Semi-rational engineering of terminal deoxynucleotidyl transferase for high-efficiency enzymatic de novo DNA synthesis [PDF]

Synthetic and Systems Biotechnology
The 21st century has witnessed rapid advancements in synthetic biology, with DNA synthesis emerging as a foundational technology. Conventional phosphoramidite-based methods face significant limitations, including short DNA elongation lengths (
Chengjie Zhang, Zhicong He, Zhengquan Gao, Chunxiao Meng, Wei Peng, Lei Du, Shengying Li   +6 more
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Terminal Deoxynucleotidyl Transferase Commonly Expresses in Germ Cell Tumors: Evaluation on a Large Series from Multiple Centers [PDF]

International Journal of General Medicine, 2021
Jun Zhou,1,* Suying Wang,2,* Lun Zhu,3 Luting Zhou,1 Hong Zeng,4 Yongli Gan,2 Chaofu Wang1 1Department of Pathology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, People’s Republic of China; 2Department of Pathology ...
Zhou J, Wang S, Zhu L, Zhou L, Zeng H, Gan Y, Wang C   +6 more
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Terminal deoxynucleotidyl transferase and CD84 identify human multi-potent lymphoid progenitors [PDF]

Nature Communications
Lymphoid specification in human hematopoietic progenitors is not fully understood. To better associate lymphoid identity with protein-level cell features, we conduct a highly multiplexed single-cell proteomic screen on human bone marrow progenitors. This
YeEun Kim, Ariel A. Calderon, Patricia Favaro, David R. Glass, Albert G. Tsai, Daniel Ho, Luciene Borges, William J. Greenleaf, Sean C. Bendall   +8 more
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Terminal Deoxynucleotidyl Transferase (TdT) Inhibiti on of Cord Blood Derived B and T Cells Expansion [PDF]

Advanced Pharmaceutical Bulletin, 2017
Purpose: Terminal deoxynucleotidyl transferase(TdT) is a DNA polymerase that is present in immature pre-B and pre-T cells. TdT inserts N-nucleotides to the V (D) J gene segment during rearrangements of genes, therefore, it plays a vital role in the ...
Sanaz Gholami, Seyede Momeneh Mohammadi, Aliakbar Movasaghpour Akbari, Ali Abedelahi, Alireza Alihemmati, Shirin Fallahi, Hojjatollah Nozad Charoudeh   +6 more
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Terminal Deoxynucleotidyl Transferase Mediated Production of Labeled Probes for Single-molecule FISH or RNA Capture [PDF]

Bio-Protocol, 2018
Arrays of short, singly-labeled ssDNA oligonucleotides enable in situ hybridization with single molecule sensitivity and efficient transcript specific RNA capture.
Imre Gáspár, Frank Wippich, Anne Ephrussi   +2 more
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Exploring structure–activity relationships of pyrrolyl diketo acid derivatives as non-nucleoside inhibitors of terminal deoxynucleotidyl transferase enzyme [PDF]

Journal of Enzyme Inhibition and Medicinal Chemistry
Terminal deoxynucleotidyl transferase (TdT) is overexpressed in some cancer types, where it drives the mutagenic repair of double strand breaks through non canonical non-homologous end joining pathway.
Valentina Noemi Madia, Nadia Garibaldi, Davide Ialongo, Elisa Patacchini, Valeria Tudino, Giuseppe Ruggieri, Laura Zarbo, Emanuele Cara, Antonio Coluccia, Marco Artico, Luigi Scipione, Antonella Messore, Francesco Saccoliti, Elisa Mentegari, Giovanni Maga, Roberto Di Santo, Emmanuele Crespan, Roberta Costi   +17 more
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