Results 71 to 80 of about 70,210 (273)

Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011

, 2008
Valverde C, Livny J, Schlüter J-P, Reinkensmeier J, Becker A, Parisi G. Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011. BMC Genomics.
Schlüter, Jan-Philip, Reinkensmeier, Jan, Valverde, Claudio, Valverde Claudio, Parisi Gustavo, Livny Jonathan, Livny, Jonathan, Parisi, Gustavo, Becker Anke, Reinkensmeier Jan, Schlüter Jan-Philip, Becker, Anke   +11 more
core   +1 more source

Improved constructs for bait RNA display in a bacterial three-hybrid assay

Scientific Reports
We have previously developed a transcription-based bacterial three-hybrid (B3H) assay as a genetic approach to probe RNA–protein interactions inside of E. coli cells.
Linh D. Nguyen, Hannah LeBlanc, Katherine E. Berry   +2 more
doaj   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

WebCARMA: a web application for the functional and taxonomic classification of unassembled metagenomic reads

, 2009
Gerlach W, Jünemann S, Tille F, Goesmann A, Stoye J. WebCARMA: a web application for the functional and taxonomic classification of unassembled metagenomic reads. BMC Bioinformatics.
Jünemann, Sebastian, Stoye Jens, Sebastian Jünemann, Goesmann, Alexander ; https://orcid.org/, Goesmann Alexander, Tille Felix, Gerlach Wolfgang, Jens Stoye, Wolfgang Gerlach, Gerlach, Wolfgang, Stoye, Jens ; https://orcid.org/, Jünemann Sebastian, Tille, Felix, Felix Tille, Alexander Goesmann   +14 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

The structure and function of the replication terminator protein of Bacillus subtilis: identification of the 'winged helix' DNA-binding domain.

, 1996
The replication terminator protein (RTP) of Bacillus subtilis impedes replication fork movement in a polar mode upon binding as two interacting dimers to each of the replication termini.
Bastia, Deepak, Wang, Fenggang, Hutchison, Clyde A., III, Pai, Karnire S., White, Stephen W., Bussiere, Dirksen E.   +5 more
core   +1 more source

Metabolic and plasmid engineering to produce D-phenyllactic acid from glucose–xylose co-substrates in Escherichia coli

Applied and Environmental Microbiology
Plasmid DNA comprises various modules, including promoter and terminator regions, replication origins, and antibiotic resistance genes as selection markers.
Shuhei Noda, Yutaro Mori, Akihiko Kondo, Daisuke Nonaka, Mayumi Dainin, Ryosuke Fujiwara, Tsutomu Tanaka, Michihiro Araki, Tomokazu Shirai   +8 more
doaj   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Pharmacological inhibition of the PERK pathway modulates hepatocellular carcinoma growth and immune signaling

FEBS Open Bio, EarlyView.
Pharmacological inhibition of PERK in a DEN‐induced mouse model of liver cancer does not reduce tumor burden but alters cellular stress signaling. Despite blocking PERK activity, downstream stress responses, including CHOP expression, remain active, suggesting compensatory mechanisms within the unfolded protein response that may influence tumor ...
Ada Lerma‐Clavero, Maria Kopsida, Nathalie Arendt, Hans Lennernäs, Markus Sjöblom, Femke Heindryckx   +5 more
wiley   +1 more source