Results 61 to 70 of about 798,978 (348)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Protective role of salt in catalysis and maintaining structure of halophilic proteins against denaturation

Frontiers in Microbiology, 2014
Search for new enzymes of industrial relevance, bestowed with novel properties continues to be a desirable pursuit in enzyme research. Halophilism is the unusual existence of life in saline/ hypersaline habitats and haloenzymes, are the proteins from ...
Rajeshwari eSinha, Sunil Kumar Khare
doaj   +1 more source

A Hierarchical Approach to Protein Molecular Evolution [PDF]

, 1999
Biological diversity has evolved despite the essentially infinite complexity of protein sequence space. We present a hierarchical approach to the efficient searching of this space and quantify the evolutionary potential of our approach with Monte Carlo ...
Crameri, Cwirla, Davidson, Devlin, Fisch, Gilbert, Gilbert, Gram, Griffiths, Hawkins, Kamtekar, Kauffman, Kauffman, Kepler, L. D. Bogarad, Lawrence, M. W. Deem, Maeshiro, Mandecki, Moore, Netzer, Patten, Pennisi, Perelson, Riddle, Scott, Shapiro, Shapiro, Stemmer, Stemmer, Zhang   +30 more
core   +2 more sources

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Single-molecule pulling: phenomenology and interpretation

, 2013
Single-molecule pulling techniques have emerged as versatile tools for probing the noncovalent forces holding together the secondary and tertiary structure of macromolecules. They also constitute a way to study at the single-molecule level processes that
Allinger, Ashkin, Basu, Berendsen, Bustamante, Camden, Chodera, Crooks, Evans, Ferrenberg, Franco, Franco, Franco, Frenkel, Freund, Gebhardt, Gunari, Halperin, Harris, Harvey, Hess, Heymann, Hoover, Hub, Huguet, Hummer, Hummer, Hummer, Imparato, Isralewitz, Izrailev, Jarzynski, Jarzynski, Jarzynski, Jarzynski, Kim, Kirkwood, Kirmizialtin, Kreuzer, Kubo, Kumar, Lee, Liphardt, Liphardt, Marsili, Martyna, McCullagh, Minh, Moerner, Neuman, Newman, Nitzan, Nosé, Oberhauser, Park, Phillips, Pohorille, Rief, Ritort, Roux, Rubinstein, Schäfer, Strick, Süzen, Thijssen, Weiss   +65 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Comparative functional genomics approach for the annotation of proteins in Unclassified Halophilic archaeon DL31 [PDF]

, 2012
The structure, function and sub-cellular location prediction for the unknown proteins from Unclassified Halophilic archaeon DL31 were carried out for characterization of the proteins in their respective families.
E Nakkeeran, N S. Sardeshmukh, S G. Sanmukh   +2 more
core   +1 more source

A study to assess the effectiveness of structure teaching programme regarding the knowledge and attitude of dietary management among patients undergoing hemodialysis at selected tertiary care hospital [PDF]

, 2023
Nishitha. ODDI, DR. Rajaram K G, Y Raveendra., Nirmala Ginkala, Maryline Flinsi D   +4 more
openalex   +1 more source

Chymotrypsin: Tertiary Structure and Enzymatic Activity [PDF]

Biochemical Journal, 1968
The stereochemistry of the active centre region of α-chymotrypsin has now been delineated in fairly accurate detail (1,2). The active serine (195) is found to be involved in a hydrogen-binding system which links it through the imidazole ring of histidine-57 to the buried carboxyl group of aspartate-102. This is believed to act as a “charge relay system”
openaire   +2 more sources

Tertiary Structural Propensities Reveal Fundamental Sequence/Structure Relationships [PDF]

Structure, 2015
Extracting useful generalizations from the continually growing Protein Data Bank (PDB) is of central importance. We hypothesize that the PDB contains valuable quantitative information on the level of local tertiary structural motifs (TERMs). We show that by breaking a protein structure into its constituent TERMs, and querying the PDB to characterize ...
Zheng, Fan, Zhang, Jian, Grigoryan, Gevorg   +2 more
openaire   +2 more sources