Expression of multiple transgenes from a single construct using viral 2A peptides in Drosophila. [PDF]
PLoS ONE, 2014 Expression of multiple reporter or effector transgenes in the same cell from a single construct is increasingly necessary in various experimental paradigms.
Richard W Daniels +3 more
doaj +1 more source
Rapid production of a chimeric antibody-antigen fusion protein based on 2A-peptide cleavage and green fluorescent protein expression in CHO cells [PDF]
, 2019 To enable large-scale antibody production, the creation of a stable, high producer cell line is essential. This process often takes longer than 6 months using standard limited dilution techniques and is very labor intensive.
Cox, Eric +2 more
core +2 more sources
Viruses, 2021 Diarrhoea and poor growth among growing pigs is responsible for significant economic losses in pig herds globally and can have a wide range of possible aetiologies.
Tarka Raj Bhatta +2 more
doaj +1 more source
MultiBac: expanding the research toolbox for multiprotein complexes [PDF]
, 2012 This article is made available for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source.
Berger, Imre +3 more
core +1 more source
Linkage map of protein–protein interactions of Porcine teschovirus
Journal of General Virology, 2005 A yeast two-hybrid study was conducted to catalogue the protein–protein interactions of the Porcine teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as ...
Roland, Zell +4 more
openaire +2 more sources
Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells. [PDF]
, 2016 Pluripotent stem cells offer great therapeutic promise for personalized treatment platforms for numerous injuries, disorders, and diseases. Octamer-binding transcription factor 4 (OCT4) is a key regulatory gene maintaining pluripotency and self-renewal ...
Awe, Jason P +9 more
core +3 more sources
Complete Coding Genome Sequence of a Putative Novel Teschovirus Serotype 12 Strain [PDF]
Genome Announcements, 2016 ABSTRACT Porcine teschoviruses are ubiquitous and prevalent viruses generally harmless to their hosts, the suids. Here, we report the first complete coding genome sequence of a putative new serotype of porcine teschovirus (PTV-12), strain CC25, isolated from fecal material from a healthy pig in Spain.
Cano-Gómez, C., Jiménez-Clavero, M. A.
openaire +3 more sources
Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
Frontiers in Microbiology, 2020 Eukaryotic microorganisms use monocistronic mRNAs to encode proteins. For synthetic biological approaches like metabolic engineering, precise co-expression of several proteins in space and time is advantageous.
Kira Müntjes +7 more
doaj +1 more source
Development of a Human Cytomegalovirus (HCMV)-Based Therapeutic Cancer Vaccine Uncovers a Previously Unsuspected Viral Block of MHC Class I Antigen Presentation [PDF]
, 2019 Human cytomegalovirus (HCMV) induces a uniquely high frequency of virus-specific effector/memory CD8+ T-cells, a phenomenon termed “memory inflation”.
Abate +109 more
core +1 more source
Enhancement of heterologous gene expression in Flammulina velutipes using polycistronic vectors containing a viral 2A cleavage sequence. [PDF]
PLoS ONE, 2013 Agrobacterium tumefaciens-mediated transformation for edible mushrooms has been previously established. However, the enhancement of heterologous protein production and the expression of multi-target genes remains a challenge.
Yu-Ju Lin +2 more
doaj +1 more source