Results 61 to 70 of about 1,827 (177)

A Comprehensive Multiomics Approach Illuminates the Biosynthetic Mechanism of Scoparone in Artemisia capillaris

open access: yesPlant Biotechnology Journal, Volume 24, Issue 1, Page 346-360, January 2026.
ABSTRACT Artemisia capillaris, a traditional Chinese medicinal plant, has displayed favourable effects in the treatment of jaundice, inflammation of the liver and cholecystitis for thousands of years. However, the biosynthesis of scoparone, the major active component in A. capillaris, remains unclear.
Fuyou Guo   +15 more
wiley   +1 more source

Rationally Designed TadA‐Derived Cytosine Editors Enable Context‐Independent Zebrafish Genome Editing

open access: yesAdvanced Science, Volume 12, Issue 39, October 20, 2025.
A next‐generation cytosine base editor (zTadCBE) and other variants are developed by combining the high‐efficiency (TadCBEa) and low‐indel (TadCBEMax) architectures, followed by the introduction of zebrafish‐optimized mutations (V82S, Q154R) in the TadA domain.
Wei Qin   +7 more
wiley   +1 more source

Unique characteristics of a picornavirus internal ribosome entry site from the porcine teschovirus-1 Talfan

open access: yes, 2002
The teschoviruses constitute a recently defined picornavirus genus. Most of the genome sequence of the porcine teschovirus-1 (PTV) Talfan and several other strains is known. We now demonstrate that initiation of protein synthesis occurs at nucleotide (nt)
Belsham, Graham J.   +3 more
core   +1 more source

Metagenomic analysis of the RNA fraction of the fecal virome indicates high diversity in pigs infected by porcine endemic diarrhea virus in the United States

open access: yesVirology Journal, 2018
Background Emergence and re-emergence of porcine epidemic diarrhea virus (PEDV) in North America, Asia and Europe has caused severe economic loss to the global swine industry.
Qi Chen   +9 more
doaj   +1 more source

Comparative analysis of two newly established Cre rat lines, NeuN‐Cre and Thy1‐Cre, for neurological research

open access: yesAnimal Models and Experimental Medicine, Volume 8, Issue 8, Page 1468-1479, August 2025.
We generated two neuron‐specific Cre rat lines, Thy1‐Cre and NeuN‐Cre, using CRISPR/Cas9‐mediated targeted insertion. Both lines were validated for high neural specificity by crossing them with a Cre reporter rat. When bred with an ND1 conditional knockout rat, the two Cre lines produced distinct phenotypic outcomes. These rat lines have been deposited
Keru Li   +15 more
wiley   +1 more source

Phylogenetic analysis of hepatovirus KSA215, teschovirus KSA412, and Crohivirus KSA403, and recombination analysis of hepatovirus KSA 215.

open access: yes, 2019
(A) Phylogenetic analysis of KSA215 bat hepatovirus based on amino acid sequences of complete polyprotein. (B) Simplot analysis using the Recombination Analysis Tool (RAT), illustrating amino acid homology between aligned sequences in a sliding window of
Komal Jain (432364)   +15 more
core   +1 more source

Exploring the Potential and Advancements of Circular RNA Therapeutics

open access: yesExploration, Volume 5, Issue 4, August 2025.
Given the remarkable advantages in terms of stability, sustained expression profile, safety, wide range of druggable targets, scalable and cost‐effective manufacturing capabilities, circRNA is currently undergoing intensive investigation for various therapeutic applications such as vaccines, protein replacement, genetic disease treatment, gene therapy,
Lei Wang   +6 more
wiley   +1 more source

Development of a reverse transcription loop-mediated isothermal amplification assay for detection of Porcine teschovirus

open access: yes, 2011
Loop-mediated isothermal amplification (LAMP) is a sensitive method for DNA amplification. In the present report, the development of a single-tube, one-step, real-time accelerated reverse transcription (RT)-LAMP for the detection of Porcine teschovirus ...
Bin Wang   +5 more
core   +1 more source

The complete genome of klassevirus – a novel picornavirus in pediatric stool

open access: yesVirology Journal, 2009
Background Diarrhea kills 2 million children worldwide each year, yet an etiological agent is not found in approximately 30–50% of cases. Picornaviral genera such as enterovirus, kobuvirus, cosavirus, parechovirus, hepatovirus, teschovirus, and ...
Ganem Donald   +6 more
doaj   +1 more source

A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae

open access: yesThe Plant Journal, Volume 122, Issue 6, June 2025.
Significance Statement Protein tags for detection, purification and fluorescence labelling are vital in microalgal research, yet the suitability of specific tags remains largely unexplored. This review discusses the pros and cons of a comprehensive list of protein tags and provides important guidance for experimental design within a microalgal context.
Jonathan Scarfe   +2 more
wiley   +1 more source

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