Results 41 to 50 of about 1,827 (177)
Expression of multiple transgenes from a single construct using viral 2A peptides in Drosophila. [PDF]
PLoS ONE, 2014 Expression of multiple reporter or effector transgenes in the same cell from a single construct is increasingly necessary in various experimental paradigms.
Richard W Daniels +3 more
doaj +1 more source
Linkage map of protein–protein interactions of Porcine teschovirus
Journal of General Virology, 2005 A yeast two-hybrid study was conducted to catalogue the protein–protein interactions of the Porcine teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as ...
Roland, Zell +4 more
openaire +2 more sources
Viruses, 2021 Diarrhoea and poor growth among growing pigs is responsible for significant economic losses in pig herds globally and can have a wide range of possible aetiologies.
Tarka Raj Bhatta +2 more
doaj +1 more source
Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
Frontiers in Microbiology, 2020 Eukaryotic microorganisms use monocistronic mRNAs to encode proteins. For synthetic biological approaches like metabolic engineering, precise co-expression of several proteins in space and time is advantageous.
Kira Müntjes +7 more
doaj +1 more source
Enhancement of heterologous gene expression in Flammulina velutipes using polycistronic vectors containing a viral 2A cleavage sequence. [PDF]
PLoS ONE, 2013 Agrobacterium tumefaciens-mediated transformation for edible mushrooms has been previously established. However, the enhancement of heterologous protein production and the expression of multi-target genes remains a challenge.
Yu-Ju Lin +2 more
doaj +1 more source
The Avian Encephalomyelitis Virus (AEV) 5’ Untranslated Region Contains an IRES with Similarity to the Hepatitis C Virus IRES [PDF]
Iranian Journal of Public Health, 2005 Cap-independent internal initiation of protein synthesis has been shown to occur on a number of viral and cellular mRNAs. Initiation of protein synthesis is directed by an internal ribosome entry site (IRES) element within the 5’ untranslated region (UTR)
M Bakhshesh, L Roberts, G J Belsham
doaj +2 more sources
Congenital Microphthalmic Syndrome in a Swine
Case Reports in Veterinary Medicine, 2018 A 17-week-old crossbred finishing pig was presented for lameness of approximately one week. Clinical evaluation, including ophthalmologic examination, revealed ataxia, partial flaccid paresis of the pelvic limbs, skin lesions at feet and claws, and ...
Radka Andrysikova +7 more
doaj +1 more source
SPLICEIT Fluorescent Sensor for Integrating Dopamine Release with Cellular Resolution
Angewandte Chemie, Volume 137, Issue 33, August 11, 2025.“Single‐chain protein‐based ligand indication through a chimerically‐engineered integrator tool” (SPLICEIT) is a G‐protein coupled receptor‐based sensor design that leaves a permanent fluorescent mark. We show dopamine detection in primary cultured neurons with a fourfold signal increase normalized to an mCherry (mCh) marker.
Steven M. Havens +7 more
wiley +2 more sources
New Phytologist, Volume 250, Issue 5, Page 3283-3298, June 2026.Summary Plant resistance influences the host range of pathogens; however, the mechanism driving this influence remains poorly understood. Brassicaceae species are not natural hosts of potexviruses, for reasons that have not yet been determined. This study investigated the molecular basis of nonhost resistance (NHR) to potexviruses in the Brassicaceae ...
Takumi Suzuki +11 more
wiley +1 more source
Microbiology Spectrum, 2023 The production of enterovirus virus-like particles (VLPs) that lack the viral genome have great potential as vaccines for a number of diseases, such as poliomyelitis and hand, foot, and mouth disease.
Lee Sherry +7 more
doaj +1 more source