Results 71 to 80 of about 1,951 (164)

A game of tag: A review of protein tags for the successful detection, purification and fluorescence labelling of proteins expressed in microalgae

The Plant Journal, Volume 122, Issue 6, June 2025.
Significance Statement Protein tags for detection, purification and fluorescence labelling are vital in microalgal research, yet the suitability of specific tags remains largely unexplored. This review discusses the pros and cons of a comprehensive list of protein tags and provides important guidance for experimental design within a microalgal context.
Jonathan Scarfe, Darius Kosmützky, R. Ellen R. Nisbet   +2 more
wiley   +1 more source

No evidence of enteric viral involvement in the new neonatal porcine diarrhoea syndrome in Danish pigs

BMC Veterinary Research, 2017
Background The aim of this study was to investigate whether the syndrome New Neonatal Porcine Diarrhoea Syndrome (NNPDS) is associated with a viral aetiology.
N. B. Goecke, C. K. Hjulsager, H. Kongsted, M. Boye, S. Rasmussen, F. Granberg, T. K. Fischer, S. E. Midgley, L. D. Rasmussen, Ø. Angen, J. P. Nielsen, S. E. Jorsal, L. E. Larsen   +12 more
doaj   +1 more source

Autologous Peripheral Vγ9Vδ2 T Cell Synergizes with αβ T Cell Through Antigen Presentation and BTN3A1 Blockade in Immunotherapy of Cervical Cancer

Advanced Science, Volume 12, Issue 18, May 15, 2025.
This study provides proof‐of‐concept evidence showing that through direct killing, antigen presentation, and competitively binding to BTN3A1, Vγ9Vδ2 T cells amplified from cervical cancer (CC) patients can synergize with αβ T cells to exert anti‐CC function both in vitro and in vivo.
Min Wu, Jian Liu, Liting Liu, Yifan Yang, Hong Liu, Long Yu, Haihong Zeng, Shuo Yuan, Ruiyi Xu, Hangyu Liu, Han Jiang, Shen Qu, Liming Wang, Ying Chen, Jingyu Wang, Yuwei Zhang, Shan He, Ling Feng, Junyan Han, Wanjiang Zeng, Hui Wang, Yafei Huang   +21 more
wiley   +1 more source

Longitudinal Investigation of Brain and Spinal Cord Pericytes After Inducible PDGFRβ+ Cell Ablation in Adult Mice

Journal of Neurochemistry, Volume 169, Issue 3, March 2025.
Pericytes are essential for central nervous system vascular function, yet studying their role in adult pathologies has been challenging due to the scarcity of well‐characterized models that preserve normal vascular development. A tamoxifen‐inducible pericyte ablation model was developed to investigate pericyte dynamics after the acute loss of pericytes.
Dila Atak, Erdost Yıldız, Esra Özkan, Mohammadreza Yousefi, Ayşe Özkan, Aysu Bilge Yılmaz, Ali Burak Kızılırmak, Iman Asaad Alnajjar, Çiçek Kanar, Zeynep Lal Caan, Şakir Ümit Zeybek, Cem İsmail Küçükali, Erdem Tüzün, Yasemin Gürsoy‐Özdemir, Atay Vural   +14 more
wiley   +1 more source

Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa) of Paraná state, Brazil

Pesquisa Veterinária Brasileira, 2015
Porcine teschovirus (PTV), porcine sapelovirus (PSV), and enterovirus G (EV-G) are infectious agents specific to pig host species that are endemically spread worldwide.
Daiane G. Donin, Raquel de A. Leme, Alice F. Alfieri, Geraldo C. Alberton, Amauri A. Alfieri   +4 more
doaj   +1 more source

Biomimetic Triplet Messenger RNA Formulation in MXene Hydrogel Microneedles for Wound Healing

Aggregate, Volume 6, Issue 2, February 2025.
The current direction in the messenger RNA (mRNA) field is focused on imparting mRNA with competitive synthetic functions and delicating carrier systems. By leveraging advanced artificial intelligence technology, a novel triplet mRNA formulation has been developed and delivered via photothermal‐responsive microneedles for the treatment of diabetic ...
Ji Wang, Yu Wang, Minhui Lu, Xinyue Cao, Ming Xia, Miaoqing Zhao, Yuanjin Zhao   +6 more
wiley   +1 more source

Genome‐wide siRNA library screening identifies human host factors that influence the replication of the highly pathogenic H5N1 influenza virus

mLife, Volume 4, Issue 1, Page 55-69, February 2025.
Abstract The global dissemination of H5 avian influenza viruses represents a significant threat to both human and animal health. In this study, we conducted a genome‐wide siRNA library screening against the highly pathogenic H5N1 influenza virus, leading us to the identification of 457 cellular cofactors (441 proviral factors and 16 antiviral factors ...
Guangwen Wang, Li Jiang, Jinliang Wang, Qibing Li, Jie Zhang, Fandi Kong, Ya Yan, Yuqin Wang, Guohua Deng, Jianzhong Shi, Guobin Tian, Xianying Zeng, Liling Liu, Zhigao Bu, Hualan Chen, Chengjun Li   +15 more
wiley   +1 more source

Detection of Teschovirus type 13 from two swine herds exhibiting nervous clinical signs in growing pigs.

Transboundary and Emerging Diseases, 2018
Recently, the number of clinical reports of growing pigs showing neurological signs possibly related to viral infections has increased. The objective of this report was to describe two outbreaks of an atypical condition observed in 6- to 7-week-old pigs ...
J. Carnero, C. Prieto, L. Polledo, F. J. Martínez-Lobo   +3 more
semanticscholar   +1 more source

Antigenic and immunogenic properties of industrial strains of Porcine Teschovirus

Agroecological journal, 2015
Досліджено 90 проб матеріалів головного та спинного мозку загиблих або вимушено забитих свиней з клінічними ознаками хвороби Тешена. Виділено 28 ізолятів вірусів. На підставі досліджень біологічних, фізико-хімічних, генетичних, антигенних та імуногенних властивостей штами Porcine teschovirus Дніпровський-34 та Городнянський-31 рекомендовано для ...
openaire   +3 more sources

“Self-cleaving” 2A peptide from porcine teschovirus-1 mediates cleavage of dual fluorescent proteins in transgenic Eimeria tenella

Veterinary Research, 2016
The “self-cleaving” 2A sequence of picornavirus, which mediates ribosome-skipping events, enables the generation of two or more separate peptide products from one mRNA containing one or more “self-cleaving” 2A sequences.
Xinming Tang, Xianyong Liu, G. Tao, Mei Qin, G. Yin, Jingxia Suo, Xun Suo   +6 more
semanticscholar   +1 more source