Results 141 to 150 of about 13,656 (172)
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Purification of tetrahydrofolate dehydrogenase by affinity chromatography
Biochimica et Biophysica Acta (BBA) - Enzymology, 1972Abstract Tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate:NADP + oxidoreductase, EC 1.5.1.3; formerly known as dihydrofolate reductase) from a subline of the L1210 lymphoma has been purified to apparent homogeneity with high recovery by simple steps using agarose to which the enzyme inhibitor amethopterin was coupled. A crude (NH 4 ) 2 SO 4
J, Gauldie, B L, Hillcoat
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Tetrahydrofolate Dehydrogenase Cytochemistry and Classification of Acute Leukaemia
Acta Haematologica, 1982Bone marrow and blood smears from patients with acute lymphoblastic and myeloblastic leukaemia prior to therapy were stained for tetrahydrofolate dehydrogenase. Compared with other cytochemical tests this reaction seems to discriminate clearly between cells with myeloid and lymphoid features.
NANO, ROSANNA +3 more
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In Vitro Antimalarial Activity of Tetrahydrofolate Dehydrogenase Inhibitors
The American Journal of Tropical Medicine and Hygiene, 1984Three tetrahydrofolate dehydrogenase (dihydrofolate reductase = EC 1.5.1.3) inhibitors were tested for antimalarial activity against Plasmodium falciparum, using an in vitro radioisotopic technique. Activity of each drug was tested in both normal RPMI medium 1640 and in modified medium (containing no p-aminobenzoic acid and 2.27 X 10(-8) M folic acid ...
D G, Sixsmith +3 more
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The Histochemical Journal, 1972
Two different methods, one proposed by us (1969, 1970) and the other by Onicescuet al. (1970), for the histochemical demonstration of tetrahydrofolate dehydrogenase have been compared using as test materials frozen sections of mouse liver and blood smears of laboratory animals that had been infected experimentally with malaria.
G, Gerzeli, P, De Piceis Polver
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Two different methods, one proposed by us (1969, 1970) and the other by Onicescuet al. (1970), for the histochemical demonstration of tetrahydrofolate dehydrogenase have been compared using as test materials frozen sections of mouse liver and blood smears of laboratory animals that had been infected experimentally with malaria.
G, Gerzeli, P, De Piceis Polver
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Biochimica et Biophysica Acta (BBA) - Enzymology, 1972
Abstract Tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate:NADP + oxidoreductase, EC 1.5.1.3; formerly known as dihydrofolate reductase) from a high enzyme mutant of L1210 mouse leukemia was purified to homogeneity by a simple two step procedure involving pH 5.1 precipitation of inert protein, and affinity chromatography employing a ...
Chello, P L +3 more
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Abstract Tetrahydrofolate dehydrogenase (5,6,7,8-tetrahydrofolate:NADP + oxidoreductase, EC 1.5.1.3; formerly known as dihydrofolate reductase) from a high enzyme mutant of L1210 mouse leukemia was purified to homogeneity by a simple two step procedure involving pH 5.1 precipitation of inert protein, and affinity chromatography employing a ...
Chello, P L +3 more
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The recovery of liver tetrahydrofolate dehydrogenase activity from inhibition by methotrexate
Biochemical Pharmacology, 1969Abstract Rat liver tetrahydrofolate dehydrogenase activity in vivo recovers rapidly from inhibition by methotrexate, despite the known stability of the enzyme-inhibitor complex. Control levels of activity are usually regained within 24 hr following a single injection of methotrexate.
B G, Stanley, G E, Neal, D C, Williams
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Acta Histochemica, 1978
Some general aspects in cytochemical demonstration of the tetrahydrofolate dehydrogenase, concerning the final reaction product, are studied. Steady and variable factors are detected in a comparative study of Vertebrate hemopoiesis: the enzyme exhibits peculiar features in different cell types.
R, Nano, G, Gerzeli, P, de Piceis Polver
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Some general aspects in cytochemical demonstration of the tetrahydrofolate dehydrogenase, concerning the final reaction product, are studied. Steady and variable factors are detected in a comparative study of Vertebrate hemopoiesis: the enzyme exhibits peculiar features in different cell types.
R, Nano, G, Gerzeli, P, de Piceis Polver
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Archives of Microbiology, 2002
Recently, it has been shown that heterotrophic methylotrophic Proteobacteria contain tetrahydrofolate (H(4)F)- and tetrahydromethanopterin (H(4)MPT)-dependent enzymes. Here we report on the purification of two methylene tetrahydropterin dehydrogenases from the methylotroph Hyphomicrobium zavarzinii ZV580. Both dehydrogenases are composed of one type of
Goenrich, M. +5 more
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Recently, it has been shown that heterotrophic methylotrophic Proteobacteria contain tetrahydrofolate (H(4)F)- and tetrahydromethanopterin (H(4)MPT)-dependent enzymes. Here we report on the purification of two methylene tetrahydropterin dehydrogenases from the methylotroph Hyphomicrobium zavarzinii ZV580. Both dehydrogenases are composed of one type of
Goenrich, M. +5 more
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Archives of Biochemistry and Biophysics, 1999
10-Formyltetrahydrofolate dehydrogenase has previously been identified as a tight binding protein of the polyglutamate forms of tetrahydrofolate (R. J. Cook and C. Wagner, Biochemistry 21, 4427-4434, 1982). Each subunit contains two independently folded domains connected by a linking peptide.
Tzu Fun Fu +3 more
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10-Formyltetrahydrofolate dehydrogenase has previously been identified as a tight binding protein of the polyglutamate forms of tetrahydrofolate (R. J. Cook and C. Wagner, Biochemistry 21, 4427-4434, 1982). Each subunit contains two independently folded domains connected by a linking peptide.
Tzu Fun Fu +3 more
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Biochemistry, 1995
The bifunctional dehydrogenase/cyclohydrolase domain of the human NADP-dependent trifunctional methyleneH4folate dehydrogenase/methenylH4folate cyclohydrolase/formylH4folate synthetase (H4folate = tetrahydrofolate) catalyzes two sequential reactions involved in the interconversion of H4folate derivatives.
J N, Pelletier, R E, MacKenzie
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The bifunctional dehydrogenase/cyclohydrolase domain of the human NADP-dependent trifunctional methyleneH4folate dehydrogenase/methenylH4folate cyclohydrolase/formylH4folate synthetase (H4folate = tetrahydrofolate) catalyzes two sequential reactions involved in the interconversion of H4folate derivatives.
J N, Pelletier, R E, MacKenzie
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