Large-scale selection synchrony of Tetrahymena thermophila [PDF]
Journal of Cell Science, 1986 ABSTRACT A method is described, based on the phagocytosis of colloidal ferrite particles, which gives highly synchronous populations of Tetrahymena thermophila. To ensure a successful synchrony, the cell culture doubling time, the limits of the phagocytic period and the distribution of cell stages must first be determined.
R J, Hill +3 more
openaire +2 more sources
Exploring the Frontiers of Cell Temperature Measurement and Thermogenesis
Advanced Science, Volume 12, Issue 1, January 9, 2025.This review focuses on the mechanisms of local temperature generation within cells and the methods developed for accurate temperature assessment. Contact and noncontact techniques are introduced, including infrared thermography, fluorescence thermometry, and other innovative localized temperature measurement approaches.
Hanliang Zhu +6 more
wiley +1 more source
Transformation of \u3cem\u3eTetrahymena thermophila\u3c/em\u3e with Hypermethylated rRNA Genes [PDF]
, 1988 The extrachromosomal rRNA genes (rDNA) of Tetrahymena thermophila contain 0.4% N6-methyladenine. C3 strain rDNA was isolated, hypermethylated in vitro, and microinjected into B strain host cells. Clonal cell lines were established, and transformants were
Karrer, Kathleen M., Yao, Meng-Chao
core +1 more source
Sumoylation is developmentally regulated and required for cell pairing during conjugation in Tetrahymena thermophila [PDF]
, 2015 The covalent attachment of small ubiquitin-like modifier (SUMO) to target proteins regulates numerous nuclear events in eukaryotes, including transcription, mitosis and meiosis, and DNA repair.
Chalker, Douglas L +3 more
core +2 more sources
Eco‐phenotypic feedback loops differ in multistressor environments
Ecology, Volume 106, Issue 1, January 2025.Abstract Natural communities are exposed to multiple environmental stressors, which simultaneously impact the population and trait dynamics of the species embedded within these communities. Given that certain traits, such as body size, are known to rapidly respond to environmental change, and given that they can strongly influence the density of ...
Lynn Govaert, Toni Klauschies
wiley +1 more source
Temporal Changes in the Role of Species Sorting and Evolution Determine Community Dynamics
Ecology Letters, Volume 28, Issue 1, January 2025.Evolutionary change within community members and shifts in species composition contribute to community and trait dynamics. To integrate when and how both processes structure communities, we estimated the contributions of species sorting and evolution over time (60 days) in bacterial communities of 24 species under selection by a ciliate predator ...
Julius Hoffmann +3 more
wiley +1 more source
Position Effect Takes Precedence Over Target Sequence in Determination of Adenine Methylation Patterns in the Nuclear Genome of a Eukaryote, \u3cem\u3eTetrahymena thermophila\u3c/em\u3e [PDF]
, 1998 Approximately 0.8% of the adenine residues in the macronuclear DNA of the ciliated protozoan Tetrahymena thermophila are modified to N6-methyladenine. DNA methylation is site specific and the pattern of methylation is constant between clonal cell lines ...
Karrer, Kathleen M. +1 more
core +1 more source
Biogenic amines at a low level of evolution: Production, functions and regulation in the unicellular Tetrahymena [PDF]
, 2015 The unicellular eukaryote Tetrahymena synthesize, store and secrete biogenic amines (histamine, serotonin, epinephrine, dopamine, melatonin) and also can take up amines from the milieu.
Csaba, György
core +1 more source
Unlocking the Potential of Chemically Modified Nucleic Acid Therapeutics
Advanced Therapeutics, Volume 7, Issue 11, November 2024.The advancements in nucleic acid therapeutics are highlighted, focusing on chemical modifications and bioconjugation techniques that enhance stability, binding affinity, and targeted delivery. Recent literature and clinical applications emphasize the role of backbone, ribose, base modifications, and bioconjugation strategies like N‐acetylgalactosamine (
Jingjing Gao +10 more
wiley +1 more source
Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids [PDF]
, 2016 The remodelling of organelle function is increasingly appreciated as a central driver of eukaryotic biodiversity and evolution. Kinetoplastids including Trypanosoma and Leishmania have evolved specialized peroxisomes, called glycosomes.
Bringaud, Frédéric +15 more
core +4 more sources