Results 211 to 220 of about 13,833 (240)

Transformation of Tetrahymena thermophila by Electroporation

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol describes a method for transformation of the Tetrahymena using electroporation. The vector is electroporated into cells after mating, where it is incorporated into the DNA of developing macronuclei. Because T. thermophila can be propagated indefinitely without conjugation, transformation of the macronucleus provides a way to ...
Melody T, Sweet, C David, Allis
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DNA binding proteins from Tetrahymena thermophila

Canadian Journal of Biochemistry, 1982
We have used DNA-cellulose chromatography to isolate single-strand binding proteins from Tetrahymena thermophila. Three major proteins which bind to denatured DNA-cellulose were obtained. The predominant protein has a molecular weight of 20 000 in sodium dodecyl sulfate – polyacrylamide gel electrophoresis and possesses many of the properties of the ...
N N, Tsao, R E, Pearlman
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Lysosomal enzymes produced by immobilized Tetrahymena thermophila

Applied Microbiology and Biotechnology, 1991
The ciliated protozoon Tetrahymena thermophila was immobilized for production of secreted lysosomal enzymes in two ways. Cells entrapped in solid Ca-alginate spheres survived but were unable to grow and multiply. However, when encapsulated in hollow Ca-alginate spheres Tetrahymena multiplied well, reaching 0.9 x 10(7) cells/ml.
T, Kiy, A, Tiedtke
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Tetrahymena thermophila

Published as part of Fu, Jinyu, Gao, Yunyi, Gao, Feng, Sheng, Yalan, Al-Farraj, Saleh A., Chen, Zigui & Wang, Chundi, 2024, An overview of genetic manipulation tools in the studies of ciliates * with emphasis on gene knockout * knockdown * and overexpression, pp.
Fu, Jinyu, Gao, Yunyi, Gao, Feng, Sheng, Yalan, Al-Farraj, Saleh A., Chen, Zigui, Wang, Chundi   +6 more
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Protein synthesis patterns in conjugating Tetrahymena thermophila

Experimental Cell Research, 1981
Abstract Conjugation in Tetrahymena is a developmental system which results in a synchronized reorganization of the genetic material in both mates of a pair. Mass cultures of T. thermophila are easily induced to conjugate simultaneously, thus conjugation is amenable to cytological, genetical and biochemical studies. We investigated the changes in
A, Ron, P B, Suhr-Jessen
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“Fenestrin” and Conjugation in Tetrahymena thermophila

Journal of Eukaryotic Microbiology, 1994
ABSTRACT Certain monoclonal antibodies interact with proteins of Tetrahymena thermophila found in the conjugation junction as well as around the gametic nuclei (pronuclei) of conjugating cells; they also react with the oral primordium and fission zone of vegetative cells and with the cytoproct and contractile vacuole pores of all cells.
E M, Nelsen, N E, Williams, H, Yi, J, Knaak, J, Frankel   +4 more
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Molecular cloning of profilin from Tetrahymena thermophila

Gene, 2000
The actin-binding protein profilin was isolated from Tetrahymena thermophila by affinity chromatography, and the peptide sequence was determined for part of the protein. The cDNA sequence was obtained by using the peptide sequence, reverse transcription-PCR and 5' and 3' RACE.
D E, Wilkes, J J, Otto
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The Golgi Apparatus of Tetrahymena Thermophila

Journal of Eukaryotic Microbiology, 1993
ABSTRACT. Electron microsocpic investigations reveal that the Golgi apparatus of Tetrahymena thermophila consists of numerous tiny dictyosomes, each consisting of one or two cisternae. the dictyosomes are localized predominantly in the cell cortex closely associated with the mitochondria, arranged in meridians alternating with the ciliary meridians. We
S, Kurz, A, Tiedtke
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Conjugation Involving Dividing Cells of Tetrahymena thermophila

The Journal of Protozoology, 1990
ABSTRACTFeulgen‐stained preparations of mixtures of starved Tetrahymena thermophila cells of complementary mating types have revealed an atypical form of conjugation involving cells which have completed the nuclear events of cell division, but have not undergone cytokinesis.
M A, Bliley, C T, Van Bell
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Tetrahymena thermophila Genetics: Concepts and Applications

2012
The differentiation of germline and somatic genomes in Tetrahymena thermophila results in two independent systems of genetic transmission. One is the conserved, sexual Mendelian genetics system of the germline genome. The other is a random genetic assortment mechanism, which operates in the somatic genome during asexual propagation.
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