Results 101 to 110 of about 616 (141)
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Analysis of DNA intercalating drugs by TGGE
Journal of Proteomics, 2005Temperature-gradient gel electrophoresis (TGGE) was used to study DNA-drug interactions. The results indicate that at least two classes of DNA intercalating drugs are distinguishable with respect to temperature increase: reversible and irreversible. The method offers an excellent means of visualizing the melting profile of an individual DNA topoisomer ...
Dusan Podhradský, Viktor Viglasky
exaly +3 more sources
High resolution SSCP by optimization of the temperature by transverse TGGE
Nucleic Acids Research, 1995 Xinguo Chen +2 more
exaly +4 more sources
RT-TGGE as a guide for the successful isolation of phosphonoacetate degrading bacteria [PDF]
Journal of Applied Microbiology, 2007 Use of molecular techniques for the isolation of bacteria capable of phosphonoacetate mineralization as carbon, phosphorus and energy source.RNA extracts obtained at three different stages of an enrichment selecting for phosphonoacetate degrading bacteria were reverse transcribed using 16S rRNA-specific primers, amplified and analysed by temperature ...
Geoff Mcmullan, James S G Dooley
exaly +3 more sources
DGGE/TGGE a method for identifying genes from natural ecosystems
Current Opinion in Microbiology, 1999Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population ...
Gérard Muyzer
exaly +3 more sources
Molecular analysis of mutations in thyroid tumors with TGGE
Experimental and Clinical Endocrinology & Diabetes, 2009Immunohistochemical demonstration of overexpression of the p53 protein indicates a mutational alteration of the gene. Our own investigations of 59 differentiated thyroid carcinomas revealed an overexpression in 15% of the tumors. A correlation to unfavourable tumor prognosis was found (stage I and II: 0/11 (0%); stage III: 4/26 (14%); stage IV: 5/22 ...
D, Simon +5 more
openaire +2 more sources
1998
The polymerase chain reaction (PCR) has become the basic tool for the amplification of genomic DNA fragments. In particular, the analysis of coding and regulatory regions are important as targets for the study of genetic diversity. To speed up methods for revealing variation in these sequences, new techniques have to be developed with the potential to ...
Michael Etscheid, Detlev Riesner
openaire +1 more source
The polymerase chain reaction (PCR) has become the basic tool for the amplification of genomic DNA fragments. In particular, the analysis of coding and regulatory regions are important as targets for the study of genetic diversity. To speed up methods for revealing variation in these sequences, new techniques have to be developed with the potential to ...
Michael Etscheid, Detlev Riesner
openaire +1 more source
1998
Single-strand conformation polymorphism (SSCP, see Chapter 8.5) is another powerful tool to identify point mutations in PCR products. PCR products are denatured and then renatured as single strands; most mutations lead to changes in conformation of the single strand and its gel mobility.
Michael Etscheid, Detlev Riesner
openaire +1 more source
Single-strand conformation polymorphism (SSCP, see Chapter 8.5) is another powerful tool to identify point mutations in PCR products. PCR products are denatured and then renatured as single strands; most mutations lead to changes in conformation of the single strand and its gel mobility.
Michael Etscheid, Detlev Riesner
openaire +1 more source
Probing the archaeal diversity of a mixed thermophilic bioleaching culture by TGGE and FISH
Systematic and Applied Microbiology, 2009The archaeal community present in a sample of Mixed Thermophilic Culture-B (MTC-B) from a laboratory-scale thermophilic bioleaching reactor was investigated by temperature gradient gel electrophoresis (TGGE) and fluorescence in situ hybridisation (FISH).
Mikkelsen, Deirdre +3 more
openaire +4 more sources
International Journal of Food Microbiology, 2005
In this paper new primers, annealing to the ITS2 region, were used to obtain a PCR product that was subsequently subjected to Temperature Gradient Gel Electrophoresis (TGGE) analysis. The PCR-TGGE method performed was able to distinguish Saccharomyces cerevisiae and S. paradoxus and distinguish between strains of S. cerevisiae.
Marisa Manzano +2 more
exaly +4 more sources
In this paper new primers, annealing to the ITS2 region, were used to obtain a PCR product that was subsequently subjected to Temperature Gradient Gel Electrophoresis (TGGE) analysis. The PCR-TGGE method performed was able to distinguish Saccharomyces cerevisiae and S. paradoxus and distinguish between strains of S. cerevisiae.
Marisa Manzano +2 more
exaly +4 more sources