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Tuberous sclerosis type 1: Three novel mutations detected in exon 15 by a combination of HDA and TGGE [PDF]

Human Mutation, 2000
Tuberous sclerosis (TSC) is an autosomal dominant disorder which is genetically heterogeneous with two genes, TSC1 and TSC2. TSC1 consists of 23 exons with exon 15 being the largest one comprising 559 bp. Representing 16% of the coding region exon 15 harbors 37% of the already identified point mutations in TSC1.
Karin Mayer, Hans-Dieter Rott
exaly   +3 more sources

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Modifications for the Improvement of TGGE and DGGE

1998
Adding a 20–40 base-long stretch of guanosine and cytosine residues to the 5′ end of one of the PCR primers leads to a PCR fragment with one extremely thermostable end and shifts the irreversible transition during DGGE and TGGE to a higher temperature or higher denaturant concentration, respectively (9).
Michael Etscheid, Detlev Riesner
openaire   +1 more source

Evaluation of Thermal Stability of RNA Nanoparticles by Temperature Gradient Gel Electrophoresis (TGGE) in Native Condition

Methods in Molecular Biology, 2017
Temperature gradient gel electrophoresis (TGGE) is a powerful tool used to analyze the thermal stabilities of nucleic acids. While TGGE is a decades-old technique, it has recently gained favor in the field of RNA nanotechnology, notably in assessing the thermal stabilities of RNA nanoparticles (NPs).
Daniel L Jasiński, Peixuan Guo, Emil F Khisamutdinov   +2 more
exaly   +3 more sources

[Mutation detection by PCR-TGGE].

Rinsho byori. The Japanese journal of clinical pathology, 1995
The variants in enterotoxigenic Escherichia coli (ETEC) heat-labile toxin (LT) gene were detected by temperature gradient gel electrophoresis (TGGE). 15 clinical strains isolated from patients and a wild type strain (B2C) were analyzed after the conventional PCR.
T, Shirakawa, K, Nishiyama, M, Matsuo
openaire   +1 more source

Characterization of Imidazole as a DNA Denaturant by Using TGGE of PCR Products from a Random Pool of DNA

Journal of Biochemistry, 1999
Perpendicular temperature gradient gel electrophoresis (TGGE) profiles were analyzed for PCR products from a random pool of DNA [60 nts random region flanked by two primer (20 nts) sites]. Besides a normal transition profile of a homoduplex, unique mobility transition profiles of two kinds of heteroduplex with a big internal loop were observed ...
P, Eli, T, Ariyama, K, Nishigaki, Y, Husimi   +3 more
openaire   +2 more sources

TGGE analysis of the diversity of ammonia-oxidizing and denitrifying bacteria in submerged filter biofilms for the treatment of urban wastewater

Applied Microbiology and Biotechnology, 2006
The spatial and temporal diversity of the bacterial community-forming biofilms in a pilot-scale submerged biofilter used for the treatment of urban wastewater was analyzed by a temperature-gradient gel electrophoresis (TGGE) approach. TGGE profiles based on partial sequence of the 16S rRNA gene showed that the community composition of the biofilms ...
C Calvo, J Gonzalez-Lopez, B Rodelas
exaly   +3 more sources

TGGE screening of the entireFBN1coding sequence in 126 individuals with marfan syndrome and related fibrillinopathies

Human Mutation, 2002
Mutations in the gene for fibrillin-1 (FBN1) cause Marfan syndrome (MFS), an autosomal dominant heritable disorder of connective tissue with prominent manifestations in the skeletal, ocular, and cardiovascular system. FBN1 mutations have also been identified in a series of related disorders of connective tissue collectively termed type-1 ...
Katzke, S., Booms, P., Tiecke, F., Palz, M., Pletschacher, A., Türkmen, S., Neumann, L., Pregla, R., Leitner, C., Schramm, C., Lorenz, P., Hagemeier, C., Fuchs, J., Skovby, F., Rosenberg, T., Robinson, P.   +15 more
openaire   +3 more sources

Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology

Antonie van Leeuwenhoek, 1998
Here, the state of the art of the application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology will be presented. Furthermore, the potentials and limitations of these techniques will be discussed, and it will be indicated why their use in ecological studies has become so ...
Muyzer, G., Smalla, K.
openaire   +3 more sources

TGGE and HIEF: a comparison of two methods in the detection of carriers of the Z mutation of the alpha-1-antitrypsin gene

Human Genetics, 1994
Alpha-1-antitrypsin (alpha-1-AT) deficiency can lead to juvenile liver cirrhosis and lung emphysema in adulthood. The deficiency Z allele is caused by a base transition. Temperature gradient gel electrophoresis (TGGE) and hybrid isoelectric focusing (HIEF) were used to detect carriers of the Z mutation of the alpha-1-AT gene.
A, Hinney, C, Dürr, C, Luckenbach, H, Ritter   +3 more
openaire   +2 more sources

TGGE fingerprints of picocyanobacteria of Lago Maggiore

2004
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Soldati E., Callieri C., Mascalchi C., Ventura S.   +3 more
openaire   +1 more source