Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Cover crop effects on μCT‐measured geometrical pore characteristics
Cover crops (CC) could improve soil physical, chemical, and biological properties; however, the micrometer scale quantification of geometric pore characteristics in CC soil is limited in the literature.
Lalith Mahendra Rankoth +3 more
doaj +1 more source
Over Time and Beyond Disney—Visualizing Princesses through a Comparative Study in India, Fiji, and Sweden [PDF]
Disney animated princesses are broadcasted around the world through Disney Channel and its global affiliates as well as through numerous other networks that purchase distribution rights. In an attempt to provide diversity in the last 25 years, Disney has featured nonwestern princesses such as those in Aladdin (1992), Pocahontas (1995), Mulan (1998 ...
openaire +5 more sources
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose +9 more
wiley +1 more source
Compte rendu de Ma‘afu, prince of Tonga, chief of Fiji. The life and times of Fiji’s first Tui Lau, de John Spurway [PDF]
Dans une esquisse d’« histoire contrefactuelle » (genre deja explore par Pascal avec le nez de Cleopâtre), un blogueur tongien d’ascendance fidjienne se demandait recemment « ce qui serait advenu si Ma‘afu » (vers 1825-1881) « avait tomber les chefs de Fidji » (Langi 2010), pour imposer a l’archipel le meme regime monarchique qu’a Tonga, le preservant ...
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The evolution of a moderate energy coast in Holocene time, Pacific Harbour, Viti Levu, Fiji [PDF]
Abstract In the absence of a fringing reef the Pacific Harbour coast of southern Viti Levu has evolved primarily under the influence of waves generated by the prevailing southeasterly trade winds. By c. 3800 yr B.P., a coastal embayment had been partially enclosed by the formation of a gravel storm beach ridge.
openaire +1 more source
Effect of terahertz irradiation on DNA damage repair in living cells
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno +3 more
wiley +1 more source

