Results 61 to 70 of about 73,656 (257)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

open access: yesFEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Cover crop effects on μCT‐measured geometrical pore characteristics

open access: yesAgrosystems, Geosciences & Environment, 2022
Cover crops (CC) could improve soil physical, chemical, and biological properties; however, the micrometer scale quantification of geometric pore characteristics in CC soil is limited in the literature.
Lalith Mahendra Rankoth   +3 more
doaj   +1 more source

Over Time and Beyond Disney—Visualizing Princesses through a Comparative Study in India, Fiji, and Sweden [PDF]

open access: yesSocial Sciences, 2019
Disney animated princesses are broadcasted around the world through Disney Channel and its global affiliates as well as through numerous other networks that purchase distribution rights. In an attempt to provide diversity in the last 25 years, Disney has featured nonwestern princesses such as those in Aladdin (1992), Pocahontas (1995), Mulan (1998 ...
openaire   +5 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

open access: yesFEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

open access: yesFEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose   +9 more
wiley   +1 more source

Compte rendu de Ma‘afu, prince of Tonga, chief of Fiji. The life and times of Fiji’s first Tui Lau, de John Spurway [PDF]

open access: yesJournal de la société des océanistes, 2015
Dans une esquisse d’« histoire contrefactuelle » (genre deja explore par Pascal avec le nez de Cleopâtre), un blogueur tongien d’ascendance fidjienne se demandait recemment « ce qui serait advenu si Ma‘afu » (vers 1825-1881) « avait tomber les chefs de Fidji » (Langi 2010), pour imposer a l’archipel le meme regime monarchique qu’a Tonga, le preservant ...
openaire   +1 more source

The evolution of a moderate energy coast in Holocene time, Pacific Harbour, Viti Levu, Fiji [PDF]

open access: yesNew Zealand Journal of Geology and Geophysics, 1990
Abstract In the absence of a fringing reef the Pacific Harbour coast of southern Viti Levu has evolved primarily under the influence of waves generated by the prevailing southeasterly trade winds. By c. 3800 yr B.P., a coastal embayment had been partially enclosed by the formation of a gravel storm beach ridge.
openaire   +1 more source

Effect of terahertz irradiation on DNA damage repair in living cells

open access: yesFEBS Open Bio, EarlyView.
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno   +3 more
wiley   +1 more source

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