Results 91 to 100 of about 128,506,783 (259)

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

PAN-00107860 - supporting-arm brooch of the Lower Rhine type

, 2022
This find is registered at Portable Antiquities of the Netherlands with number PAN ...
Portable Antiquities of the Netherlands, DataCurator
core   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

PAN-00002624 - one-ribbed La Tène bracelet variant H3a

, 2018
This find is registered at Portable Antiquities of the Netherlands with number PAN ...
Portable Antiquities of the Netherlands, DataCurator
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Prosperity in the Netherlands 1962

, 2007
Opinions about prosperity and welfare in country and own family / opinions about definitions of prosperity / attitude towards income distribution in the Netherlands.
Nederlands instituut voor de publieke opinie en het marktonderzoek, NIPO * Amsterdam (primary investigator)
core   +1 more source

Advocating the value-add of faith in a secular context: The case of the Knowledge Centre Religion and Development in the Netherlands

HTS Teologiese Studies/Theological Studies, 2019
This article analyses how faith-based civil society organisations have advocated the value-add of faith to governmental and non-governmental development actors in the highly secularised context of the Netherlands.
Brenda E. Bartelink, Ton Groeneweg
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Data presented in the paper "Colony-breeding Eurasian spoonbills in the Netherlands: local limits to population growth with expansion into new areas"

, 2018
The dataset contains three files, all relating to spoonbill breeding colonies in the Netherlands. We used this dataset to assess population growth of Spoonbills in the Netherlands, and especially in the Wadden Sea. The first file contains yearly breeding
Lok, T. (Tamar), Piersma, T. (Theunis), de Goeij, P. (Petra), Oudman, T. (Thomas)   +3 more
core   +1 more source

Selective elimination of quiescent cancer cells by dequalinium chloride via ROS‐mediated ferroptosis

FEBS Open Bio, EarlyView.
Dequalinium chloride (DQ) selectively eliminates quiescent cancer cells (QCCs) by amplifying mitochondrial ROS. QCCs exhibit elevated basal ROS and limited redox‐buffering capacity, making them vulnerable to further ROS increases. DQ‐induced ROS exceeds the tolerance threshold, triggering lipid peroxidation and ferroptosis, whereas proliferating cells ...
Kotaro Miyamoto, Shiori Sakai, Minori Endo, Terigele, Mizuho Niibori, Taiki Morita, Yu Goto, Arisa Yoshida, Tomohiro Tanaka, Ning Lin, Takahiro Kuchimaru, Hiroyuki Nakamura, Masahiro Inoue, Shinae Kizaka‐Kondoh, Tetsuya Kadonosono   +14 more
wiley   +1 more source