Results 51 to 60 of about 132,352,308 (329)
Although some studies have been conducted to compute fragility surfaces of buildings using vector-valued seismic intensity measures (IMs), in all the cases, the first component of the vector usually is the spectral acceleration at first mode of vibration
Noel Zavala +8 more
doaj +1 more source
THE M31 VELOCITY VECTOR. I. HUBBLE SPACE TELESCOPE PROPER-MOTION MEASUREMENTS [PDF]
We present the first proper-motion (PM) measurements for the galaxy M31. We obtained new V-band imaging data with the Hubble Space Telescope ACS/WFC and the WFC3/UVIS instruments of three fields: a spheroid field near the minor axis, an outer disk field ...
S. Sohn +2 more
semanticscholar +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Estimation of Velocity Vectors in Synthetic Aperture Ultrasound Imaging [PDF]
A method for determining both velocity magnitude and angle in a synthetic aperture ultrasound system is described. The approach uses directional beamforming along the flow direction and cross correlation to determine velocity magnitude. The angle of the flow is determined from the maximum normalized correlation calculated as a function of angle.
Jørgen Arendt Jensen, Niels Oddershede
openaire +3 more sources
Vectors as applied to Problems in Velocity [PDF]
A velocity is a vector quantity, for it possesses magnitude and direction. It is, of course, necessary to make clear in every case what given point (moving or not) is considered as fixed for the time being, to which the vclocity of the moving point (so-called, though it may only be relatively moving) is referred.
openaire +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
The Role of the Harmonic Vector Average in Motion Integration
The local speeds of object contours vary systematically with the cosine of the angle between the normal component of the local velocity and the global object motion direction.
Alan eJohnston, Peter eScarfe
doaj +1 more source
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source

