Results 191 to 200 of about 1,039,047 (309)

Case report for an intervention for scientific pressure by brief strategic withdrawal. [PDF]

Front Psychiatry
Meng L, Wu S, Wu Z.
europepmc   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

The Impact of Allergic Rhinitis on Australian Adults in an Urban Setting: A Qualitative Exploration. [PDF]

J Clin Med
Opadokun A, Mikhail E, Chong LX, Suphioğlu C, Gokhale M, Azizoğlu S.   +5 more
europepmc   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Facilitators and Barriers to Accessing and Continuing Speech Therapy for Children With Cleft Palate: A Qualitative Study. [PDF]

Int J Lang Commun Disord
Dash S, Arora P, Sinha A, Dhiman A, Manas RK, Tiwari R, Chauhan S, Sarabhai S.   +7 more
europepmc   +1 more source

Overarching Themes

Investigative Opthalmology & Visual Science, 2012
openaire   +2 more sources

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

This Is My Story (TIMS), a Five-Year Thematic Analysis of Patient Narratives from a Novel Intervention in an Academic Hospital. [PDF]

Healthcare (Basel)
Wilson J, Bains B, Rapuri S, Robitaille E, Chan C, Crane N, Fei E, Tracey E.   +7 more
europepmc   +1 more source

Theme issues

Journal of ISAKOS, 2023
openaire   +2 more sources