Results 41 to 50 of about 1,116,546 (296)

Pedagogical support for professional self-determination of high school students in IT professions

open access: yesОбразование и наука, 2021
Introduction. The formation of professional self-determination of high school students and the associated choice of vocational education is an urgent problem of the modern school.
E. K. Khenner
doaj   +1 more source

The tropical double description method [PDF]

open access: yes, 2010
We develop a tropical analogue of the classical double description method allowing one to compute an internal representation (in terms of vertices) of a polyhedron defined externally (by inequalities).
Allamigeon, Xavier   +2 more
core   +5 more sources

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

Cell‐cycle‐specific lesion evolution rather than inhibition of double‐strand‐break repair underpins cisplatin radiosensitization

open access: yesMolecular Oncology, EarlyView.
We analyze cisplatin–DNA adducts (CDAs) and double‐strand breaks (DSBs) in a cell‐cycle‐dependent manner. We find that CDAs form similarly across all cell cycle phases. DSBs arise only in S‐phase. CDAs might not directly impair DSB repair, but S‐phase DSB lesions evolve in the presence of CDAs and disrupt repair in G2, also causing radiosensitization ...
Ye Qiu   +10 more
wiley   +1 more source

Methodological comparison of agent models [PDF]

open access: yes, 1998
Hybrid agent architectures comprise the radical change of paradigms in AI over the past decades by reconciling the different styles of reactive, deliberative, even social systems.
Fischer, Klaus, Jung, Christoph G.
core   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Flow Logic [PDF]

open access: yes, 2019
Flow networks have attracted a lot of research in computer science. Indeed, many questions in numerous application areas can be reduced to questions about flow networks.
Kupferman, Orna, Vardi, Gal
core   +2 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

The place of expert systems in a typology of information systems [PDF]

open access: yes, 1986
This article considers definitions and claims of Expert Systems ( ES) and analyzes them in view of traditional Information systems (IS). It is argued that the valid specifications for ES do not differ fran those for IS. Consequently the theoretical study
Simons, J.L., Wieringa, R.J.
core   +2 more sources

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