Results 301 to 310 of about 2,723,431 (383)

Thermal denaturation assays in chemical biology.

ASSAY and Drug Development Technologies, 2012
Thermal denaturation-based methods are becoming increasingly used to characterize protein stability and interactions. Recent technical advances have made these methods more suitable for high throughput screening.
G. Senisterra, Irene Chau, M. Vedadi
semanticscholar   +4 more sources

Metamaterial Terahertz Sensor for Measuring Thermal-Induced Denaturation Temperature of Insulin

IEEE Sensors Journal, 2020
A terahertz (THz) metamaterial biosensor is proposed to measure the thermal induced denaturation temperature of the insulin, a typical protein. The unit cell of the biosensor consists of a H-shaped metal structure enclosed by a U-shaped metal structure ...
Dongxia Li   +5 more
semanticscholar   +1 more source

Nonideality and protein thermal denaturation

Biopolymers, 1999
We studied the thermal denaturation of eglin c by using CD spectropolarimetry and differential scanning calorimetry (DSC). At low protein concentrations, denaturation is consistent with the classical two-state model. At concentrations greater than several hundred microM, however, the calorimetric enthalpy and the midpoint transition temperature ...
Jennifer C. Waldner   +3 more
openaire   +3 more sources

Sugar-Mediated Stabilization of Protein against Chemical or Thermal Denaturation.

Journal of Physical Chemistry B, 2018
The protective action of sugars against the denaturation of myoglobin was clarified by X-ray and neutron scattering methods. Different types of sugars such as disaccharides (trehalose, sucrose) and monosaccharides (glucose, fructose) were used ...
Satoshi Ajito   +3 more
semanticscholar   +1 more source

Thermal denaturation of staphylococcal nuclease

Biochemistry, 1985
The fully reversible thermal denaturation of staphylococcal nuclease in the absence and presence of Ca2+ and/or thymidine 3',5'-diphosphate (pdTp) from pH 4 to 8 has been studied by high-sensitivity differential scanning calorimetry. In the absence of ligands, the denaturation is accompanied by an enthalpy change of 4.25 cal g-1 and an increase in ...
Reyna O. Calderon   +5 more
openaire   +2 more sources

Thermal denaturation of subchromosomal particles

Biochemical and Biophysical Research Communications, 1975
Summary Monomer chromatin subunits prepared by micrococcal nuclease digestion showed a monophasic thermal denaturation transition with a Tm of about 77°C. By contrast, dimers and higher oligomers gave a biphasic melting profile, with Tms at 45–55°C and 77°C.
C.L.F. Woodcock, L‐L. Y. Frado
openaire   +3 more sources

Quercetin Influence on Thermal Denaturation of Bovine Serum Albumin.

Journal of Physical Chemistry B, 2016
The effect of quercetin (QUER) binding on bovine serum albumin (BSA) thermal denaturation was systematically investigated by means of differential scanning calorimetry (DSC).
Aurica Precupas, Romică Sandu, V. Popa
semanticscholar   +1 more source

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