Results 11 to 20 of about 2,881 (155)

The pyruvate:ferredoxin oxidoreductase of the thermophilic acetogen, Thermoanaerobacter kivui [PDF]

FEBS Open Bio, 2021
Pyruvate:ferredoxin oxidoreductase (PFOR) is a key enzyme in bacterial anaerobic metabolism. Since a low‐potential ferredoxin (Fd2−) is used as electron carrier, PFOR allows for hydrogen evolution during heterotrophic growth as well as pyruvate synthesis
Alexander Katsyv, Marie Charlotte Schoelmerich, Mirko Basen, Volker Müller   +3 more
doaj   +6 more sources

Engineering the hyperthermophilic archaeon Pyrococcus furiosus for 1-propanol production [PDF]

Applied and Environmental Microbiology
Society relies heavily on chemicals traditionally produced through the refinement of fossil fuels. The conversion of renewable biomass to value-added chemicals by microbes, particularly hyperthermophiles (Topt ≥80°C), offers a renewable alternative to ...
Hailey C. O'Quinn, Jason L. Vailionis, Tania N. N. Tanwee, Katherine S. Holandez-Lopez, Ryan G. Bing, Farris L. Poole, Ying Zhang, Robert M. Kelly, Michael W. W. Adams   +8 more
doaj   +2 more sources

Dataset describing the amino acid catabolism of Thermoanaerobacter pseudethanolicus [PDF]

Data in Brief
The dataset depicts the catabolism of branched-chain amino acids by Thermoanaerobacter pseudethanolicus in the presence of thiosulfate under different culture conditions. The results reveal that the strain can degrade all three branched-chain amino acids
Johann Orlygsson, Sean Michael Scully
doaj   +2 more sources

ICECREAM: high-fidelity equivariant cryo-electron tomography. [PDF]

Acta Crystallogr D Struct Biol
We present ICECREAM, a self‐supervised approach that achieves substantially better denoising and more reliable missing‐wedge recovery in cryo‐ET, while reducing training and inference time relative to comparable baselines.Cryo‐electron tomography (cryo‐ET) visualizes 3D cellular architecture in its near‐native state.
Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I.   +4 more
europepmc   +2 more sources

Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui [PDF]

Nature Communications
Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses
Weisong Liu, Kuncheng Zhang, Jiang Liu, Yuanming Wang, Meng Zhang, Huijuan Cui, Junsong Sun, Lingling Zhang   +7 more
doaj   +2 more sources

Method for Accurately Assessing the Impact of Enzyme Encapsulation in a Protein Nanocage

Nano Select
Encapsulating enzymes within protein cages is attracting increasing attention for its potential to enhance reaction efficiency, similar to mechanisms found in nature, as well as the promise for practical applications.
Norifumi Kawakami, Mao Kitamura, Keiichi Hayashi, Eri Kiyota, Ryoichi Arai, Kenji Miyamoto   +5 more
doaj   +2 more sources

Progresses and challenges of engineering thermophilic acetogenic cell factories [PDF]

Frontiers in Microbiology
Thermophilic acetogens are gaining recognition as potent microbial cell factories, leveraging their unique metabolic capabilities to drive the development of sustainable biotechnological processes. These microorganisms, thriving at elevated temperatures,
Barbara Bourgade, M. Ahsanul Islam
doaj   +2 more sources

The energy-converting hydrogenase Ech2 is important for the growth of the thermophilic acetogen Thermoanaerobacter kivui on ferredoxin-dependent substrates [PDF]

Microbiology Spectrum
Thermoanaerobacter kivui is the thermophilic acetogenic bacterium with the highest temperature optimum (66°C) and with high growth rates on hydrogen (H2) plus carbon dioxide (CO2).
Christoph Baum, Benjamin Zeldes, Anja Poehlein, Rolf Daniel, Volker Müller, Mirko Basen   +5 more
doaj   +2 more sources

Insights into Protein and Amino Acid Metabolism of Thermoanaerobacter mathranii

Fermentation
Few investigations have been carried out into the members of the genus Thermoanerobacter for protein and amino acid metabolism compared to carbohydrates, mostly due to the intense interest in bioethanol and biohydrogen in recent decades.
Sean Michael Scully, Clay A. Abraham, Johann Orlygsson   +2 more
doaj   +2 more sources

Electrotransformation of Thermoanaerobacter ethanolicus JW200 [PDF]

Biotechnology Letters, 2006
Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette.
Hui, Peng, Bailing, Fu, Zhonggui, Mao, Weilan, Shao   +3 more
openaire   +4 more sources