Results 11 to 20 of about 3,230 (159)
Method for Accurately Assessing the Impact of Enzyme Encapsulation in a Protein Nanocage
Nano SelectEncapsulating enzymes within protein cages is attracting increasing attention for its potential to enhance reaction efficiency, similar to mechanisms found in nature, as well as the promise for practical applications.
Norifumi Kawakami +5 more
doaj +2 more sources
Electrotransformation of Thermoanaerobacter ethanolicus JW200 [PDF]
Biotechnology Letters, 2006 Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette.
Hui, Peng +3 more
openaire +4 more sources
Analysis of a Clostridium difficile PCR ribotype 078 100 kilobase island reveals the presence of a novel transposon, Tn6164. [PDF]
, 2012 Clostridium difficile is the main cause of antibiotic associated diarrhea. In the past decade, the number of C. difficile patients has increased dramatically, coinciding with the emergence of two PCR ribotypes 027 and 078.
Bakker, D +8 more
core +9 more sources
Cysteine reactivity in Thermoanaerobacter brockii alcohol dehydrogenase [PDF]
Protein Science, 1997 AbstractThe free cysteine residues in the extremely thermophilic Thermoanaerobacter brockii alcohol dehydrogenase (TBADH) were characterized using selective chemical modification with the stable nitroxyl biradical bis(1‐oxy‐2,2,5,5‐tetramethyl‐3‐imidazoline‐4‐yl)disulfide, via a thiol‐disulfide exchange reaction and with 2[14C]iodoacetic acid, via S ...
M, Peretz, L M, Weiner, Y, Burstein
openaire +2 more sources
ISME Communications, 2023 DNA uptake is widespread among microorganisms and considered a strategy for rapid adaptation to new conditions. While both DNA uptake and adaptation are referred to in the context of natural environments, they are often studied in laboratories under ...
Benjamin Zeldes +6 more
doaj +1 more source
Thermal Stability of Glucokinases inThermoanaerobacter tengcongensis [PDF]
BioMed Research International, 2013 In the genome ofThermoanaerobacter tengcongensis, three genes belonging to ROK (Repressor, ORF, and Kinase) family are annotated as glucokinases (GLKs). Using enzyme assays, the three GLKs were identified as ATP-dependent GLK (ATP-GLK), ADP-dependent GLK (ADP-GLK), and N-acetyl-glucosamine/mannosamine kinase (glu/man-NacK).
Qian, Zhong +7 more
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Microorganisms, 2021 Thermoanaerobacter species have recently been observed to reduce carboxylic acids to their corresponding alcohols. The present investigation shows that Thermoanaerobacter pseudoethanolicus converts C2–C6 short-chain fatty acids (SCFAs) to their ...
Sean Michael Scully +4 more
doaj +1 more source
Continuous Ethanol Fermentation of Pretreated Lignocellulosic Biomasses, Waste Biomasses, Molasses and Syrup Using the Anaerobic, Thermophilic Bacterium Thermoanaerobacter italicus Pentocrobe 411. [PDF]
PLoS ONE, 2015 Lignocellosic ethanol production is now at a stage where commercial or semi-commercial plants are coming online and, provided cost effective production can be achieved, lignocellulosic ethanol will become an important part of the world bio economy ...
Rasmus Lund Andersen +2 more
doaj +1 more source
A fluorescent reporter system for anaerobic thermophiles
Frontiers in Bioengineering and Biotechnology, 2023 Owing to their inherent capacity to make invisible biological processes visible and quantifiable, fluorescent reporter systems have numerous applications in biotechnology. For classical fluorescent protein systems (i.e., GFP and derivatives), chromophore
Rémi Hocq +7 more
doaj +1 more source
CO Metabolism in the Thermophilic Acetogen Thermoanaerobacter kivui [PDF]
Applied and Environmental Microbiology, 2016 ABSTRACT The thermophilic acetogenic bacterium Thermoanaerobacter kivui , previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H 2 plus CO 2 and by ...
Marie Charlotte, Weghoff +1 more
openaire +2 more sources