Progresses and challenges of engineering thermophilic acetogenic cell factories [PDF]
Thermophilic acetogens are gaining recognition as potent microbial cell factories, leveraging their unique metabolic capabilities to drive the development of sustainable biotechnological processes. These microorganisms, thriving at elevated temperatures,
Barbara Bourgade, M. Ahsanul Islam
doaj +2 more sources
Method for Accurately Assessing the Impact of Enzyme Encapsulation in a Protein Nanocage
Encapsulating enzymes within protein cages is attracting increasing attention for its potential to enhance reaction efficiency, similar to mechanisms found in nature, as well as the promise for practical applications.
Norifumi Kawakami +5 more
doaj +2 more sources
The energy-converting hydrogenase Ech2 is important for the growth of the thermophilic acetogen Thermoanaerobacter kivui on ferredoxin-dependent substrates [PDF]
Thermoanaerobacter kivui is the thermophilic acetogenic bacterium with the highest temperature optimum (66°C) and with high growth rates on hydrogen (H2) plus carbon dioxide (CO2).
Christoph Baum +5 more
doaj +2 more sources
Cysteine reactivity in Thermoanaerobacter brockii alcohol dehydrogenase [PDF]
AbstractThe free cysteine residues in the extremely thermophilic Thermoanaerobacter brockii alcohol dehydrogenase (TBADH) were characterized using selective chemical modification with the stable nitroxyl biradical bis(1‐oxy‐2,2,5,5‐tetramethyl‐3‐imidazoline‐4‐yl)disulfide, via a thiol‐disulfide exchange reaction and with 2[14C]iodoacetic acid, via S ...
Moshe Peretz, Lev Weiner, Yigal Burstein
openalex +4 more sources
Description of Thermoanaerobacter brockii subsp. lactiethylicus subsp. nov., Isolated from a Deep Subsurface French Oil Well, a Proposal To Reclassify Thermoanaerobacter finnii as Thermoanaerobacter brockii subsp. finnii comb. nov., and an Emended Description of Thermoanaerobacter brockii [PDF]
A strictly anaerobic, thermophilic, gram-positive, spore-forming cubacterium designated strain SERB 5268T (T = type strain) was isolated from an oil field at a depth of 2,100 m, where the temperature was 92 degrees C. The cells of this organism were gram-positive, straight, motile rods (0.5 by 2 to 3 microns) with peritrichous flagella.
Cayol, J. L. +7 more
openaire +6 more sources
Analysis of a Clostridium difficile PCR ribotype 078 100 kilobase island reveals the presence of a novel transposon, Tn6164. [PDF]
Clostridium difficile is the main cause of antibiotic associated diarrhea. In the past decade, the number of C. difficile patients has increased dramatically, coinciding with the emergence of two PCR ribotypes 027 and 078.
Bakker, D +8 more
core +9 more sources
Draft genome sequence of rhamnolipid-producing bacteria Thermoanaerobacter thermocopriae strain CM-CNRG TB177 isolated from an oil reservoir in Mexico. [PDF]
Segovia V +2 more
europepmc +2 more sources
Electrotransformation of Thermoanaerobacter ethanolicus JW200 [PDF]
Electrotransformation of Thermoanaerobacter ethanolicus JW200 was achieved using the plasmid, pTE16, and a pUC-based suicide vector, pTEA2. The construct pTE16 is based on the Escherichia coli-Clostridium perfringens shuttle vector pJIR715 and contains a thermostable chloramphenicol (Cm) resistance cassette.
Hui, Peng +3 more
openaire +4 more sources
DNA uptake is widespread among microorganisms and considered a strategy for rapid adaptation to new conditions. While both DNA uptake and adaptation are referred to in the context of natural environments, they are often studied in laboratories under ...
Benjamin Zeldes +6 more
doaj +1 more source
Thermal Stability of Glucokinases inThermoanaerobacter tengcongensis [PDF]
In the genome ofThermoanaerobacter tengcongensis, three genes belonging to ROK (Repressor, ORF, and Kinase) family are annotated as glucokinases (GLKs). Using enzyme assays, the three GLKs were identified as ATP-dependent GLK (ATP-GLK), ADP-dependent GLK (ADP-GLK), and N-acetyl-glucosamine/mannosamine kinase (glu/man-NacK).
Qian, Zhong +7 more
openaire +2 more sources

