Hi-TARGET: a fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui. [PDF]
Biotechnol Biofuels BioprodDue to its ability to grow fast on CO2, CO and H2 at high temperatures and with high energy efficiency, the thermophilic acetogen Thermoanaerobacterkivui could become an attractive host for industrial biotechnology.
Sitara A, Hocq R, Lu AJ, Pflügl S.
europepmc +3 more sources
A fluorescent reporter system for anaerobic thermophiles [PDF]
Frontiers in Bioengineering and Biotechnology, 2023 Owing to their inherent capacity to make invisible biological processes visible and quantifiable, fluorescent reporter systems have numerous applications in biotechnology. For classical fluorescent protein systems (i.e., GFP and derivatives), chromophore
Rémi Hocq +7 more
doaj +2 more sources
Obligate autotrophy at the thermodynamic limit of life in a new acetogenic bacterium [PDF]
Frontiers in Microbiology, 2023 One of the important current issues of bioenergetics is the establishment of the thermodynamic limits of life. There is still no final understanding of what is the minimum value of the energy yield of a reaction that is sufficient to be used by an ...
Evgenii N. Frolov +6 more
doaj +2 more sources
The monofunctional CO dehydrogenase CooS is essential for growth of Thermoanaerobacter kivui on carbon monoxide [PDF]
Extremophiles, 2021 Thermoanaerobacter kivui is a thermophilic acetogen that can grow on carbon monoxide as sole carbon and energy source. To identify the gene(s) involved in CO oxidation, the genome sequence was analyzed.
Surbhi Jain +3 more
openalex +2 more sources
Microorganisms, 2021 Thermoanaerobacter species have recently been observed to reduce carboxylic acids to their corresponding alcohols. The present investigation shows that Thermoanaerobacter pseudoethanolicus converts C2–C6 short-chain fatty acids (SCFAs) to their ...
Sean Michael Scully +4 more
doaj +2 more sources
Electron carriers involved in autotrophic and heterotrophic acetogenesis in the thermophilic bacterium Thermoanaerobacter kivui [PDF]
Extremophiles, 2021 Thermoanaerobacter kivui is an acetogenic model organism that reduces CO 2 with electrons derived from H 2 or CO, or from organic substrates in the Wood–Ljugdahl pathway (WLP).
Alexander Katsyv +3 more
semanticscholar +3 more sources
Engineering the hyperthermophilic archaeon Pyrococcus furiosus for 1-propanol production [PDF]
Applied and Environmental MicrobiologySociety relies heavily on chemicals traditionally produced through the refinement of fossil fuels. The conversion of renewable biomass to value-added chemicals by microbes, particularly hyperthermophiles (Topt ≥80°C), offers a renewable alternative to ...
Hailey C. O'Quinn +8 more
doaj +2 more sources
Proteins: Structure, Function, and Bioinformatics, 2022 Three‐dimensional structures of I86A and C295A mutant secondary alcohol dehydrogenase (SADH) from Thermoanaerobacter pseudoethanolicus were determined by x‐ray crystallography.
Tung Dinh +2 more
openalex +3 more sources
A purified energy-converting hydrogenase from Thermoanaerobacter kivui demonstrates coupled H+-translocation and reduction in vitro [PDF]
Journal of Biological Chemistry, 2022 Alexander Katsyv, Volker Müller
openalex +2 more sources
Insights into Protein and Amino Acid Metabolism of Thermoanaerobacter mathranii
FermentationFew investigations have been carried out into the members of the genus Thermoanerobacter for protein and amino acid metabolism compared to carbohydrates, mostly due to the intense interest in bioethanol and biohydrogen in recent decades.
Sean Michael Scully +2 more
doaj +2 more sources