Results 1 to 10 of about 1,487 (124)

The pyruvate:ferredoxin oxidoreductase of the thermophilic acetogen, Thermoanaerobacter kivui [PDF]

FEBS Open Bio, 2021
Pyruvate:ferredoxin oxidoreductase (PFOR) is a key enzyme in bacterial anaerobic metabolism. Since a low‐potential ferredoxin (Fd2−) is used as electron carrier, PFOR allows for hydrogen evolution during heterotrophic growth as well as pyruvate synthesis
Alexander Katsyv, Marie Charlotte Schoelmerich, Mirko Basen, Volker Müller   +3 more
doaj   +11 more sources

Revealing formate production from carbon monoxide in wild type and mutants of Rnf‐ and Ech‐containing acetogens, Acetobacterium woodii and Thermoanaerobacter kivui [PDF]

Microbial Biotechnology, 2020
Summary Acetogenic bacteria have gained much attraction in recent years as they can produce different biofuels and biochemicals from H2 plus CO2 or even CO alone, therefore opening a promising alternative route for the production of biofuels from ...
Fabian M. Schwarz, Sarah Ciurus, Surbhi Jain, Christoph Baum, Anja Wiechmann, Mirko Basen, Volker Müller   +6 more
doaj   +9 more sources

A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide [PDF]

Nature Communications
Acetogens are promising industrial biocatalysts for upgrading syngas, a gas mixture containing CO, H2 and CO2 into fuels and chemicals. However, CO severely inhibits growth of many acetogens, often requiring extensive adaptation to enable efficient CO ...
Rémi Hocq, Josef Horvath, Maja Stumptner, Mykolas Malevičius, Gerhard G. Thallinger, Stefan Pflügl   +5 more
doaj   +7 more sources

Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui [PDF]

Nature Communications
Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses
Weisong Liu, Kuncheng Zhang, Jiang Liu, Yuanming Wang, Meng Zhang, Huijuan Cui, Junsong Sun, Lingling Zhang   +7 more
doaj   +5 more sources

Non-canonical resource allocation in heterotrophically growing Thermoanaerobacter kivui [PDF]

Nature Communications
Allocation of resources in the costly proteome reflects trade-offs between cellular functions. For example, proteome composition of Escherichia coli is significantly regulated by growth rate.
Franziska Maria Mueller, Albert Leopold Müller, Wenyu Gu, Farshad Abdollah-Nia, Jiawei Sun, Jenna Kim Ahn, Kerwyn Casey Huang, James R. Williamson, Alfred Michael Spormann   +8 more
doaj   +4 more sources

A fluorescent reporter system for anaerobic thermophiles [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Owing to their inherent capacity to make invisible biological processes visible and quantifiable, fluorescent reporter systems have numerous applications in biotechnology. For classical fluorescent protein systems (i.e., GFP and derivatives), chromophore
Rémi Hocq, Rémi Hocq, Sara Bottone, Sara Bottone, Arnaud Gautier, Arnaud Gautier, Stefan Pflügl, Stefan Pflügl   +7 more
doaj   +6 more sources

A thermostable Cas9-based genome editing system for thermophilic acetogenic bacterium Thermoanaerobacter kivui [PDF]

Applied and Environmental Microbiology
Thermoanaerobacter kivui is a thermophilic acetogenic bacterium capable of thriving at elevated temperatures up to 66°C. It metabolizes carbohydrates such as glucose, mannose, and fructose and can also grow lithotrophically utilizing hydrogen (H2) and ...
Yilin Le, Xue Liu, Shidong Zhou, Pengju Wu, Mengqi Zhang, Jianzhong Sun, Jinfeng Ni, Huilei Wang   +7 more
doaj   +5 more sources

Hi-TARGET: a fast, efficient and versatile CRISPR type I-B genome editing tool for the thermophilic acetogen Thermoanaerobacter kivui [PDF]

Biotechnology for Biofuels and Bioproducts
Background Due to its ability to grow fast on CO2, CO and H2 at high temperatures and with high energy efficiency, the thermophilic acetogen Thermoanaerobacter kivui could become an attractive host for industrial biotechnology.
Angeliki Sitara, Rémi Hocq, Alexander Jiwei Lu, Stefan Pflügl   +3 more
doaj   +6 more sources

Formate Is Required for Growth of the Thermophilic Acetogenic Bacterium Thermoanaerobacter kivui Lacking Hydrogen-Dependent Carbon Dioxide Reductase (HDCR) [PDF]

Frontiers in Microbiology, 2020
The hydrogen-dependent carbon dioxide reductase is a soluble enzyme complex that directly utilizes hydrogen (H2) for the reduction of carbon dioxide (CO2) to formate in the first step of the acetyl-coenzyme A- or Wood-Ljungdahl pathway (WLP).
Surbhi Jain, Helge M. Dietrich, Volker Müller, Mirko Basen   +3 more
doaj   +7 more sources

Sugar-inducible promoters for manipulation of core metabolic pathways in the thermophilic acetogen Thermoanaerobacter kivui [PDF]

Applied and Environmental Microbiology
Acetogenic bacteria form an essential part of the global carbon cycle and show promise for green chemicals production thanks to their energy-efficient carbon fixation via the Wood-Ljungdahl pathway. Energy conservation in acetogens is dependent on one of
Benjamin Zeldes, Sabina Mittelstedt, Christoph Baum, Adilia Shakirova, Anja Poehlein, Rolf Daniel, Mirko Basen   +6 more
doaj   +5 more sources