Results 101 to 110 of about 502 (114)

[3H]GDP binding and thermogenin amount in brown adipose tissue mitochondria from cold-exposed rats.

American Journal of Physiology-Cell Physiology, 1985
Brown fat mitochondria were isolated from cold-exposed and control rats, and their content of the brown-fat-specific 32-kDa “uncoupling” protein thermogenin determined both by the traditional [3H]GDP-binding method and by the recently developed enzyme-linked immunosorbent assay (ELISA).
J. Nedergaard, B. Cannon
semanticscholar   +3 more sources

Chapter 17 The uncoupling protein thermogenin and mitochondrial thermogenesis

, 1992
Publisher Summary This chapter discusses the uncoupling protein thermogenin and mitochondrial thermogenesis. The activity of thermogenin has traditionally been studied in isolated brown-fat mitochondria, however it has become possible to isolate thermogenin itself, and this has opened new avenues for thermogenesis research.
J. Nedergaard, B. Cannon
semanticscholar   +2 more sources

Mitochondrial thermogenin content is unchanged during atrophy of BAT of fasting mice.

American Journal of Physiology-Endocrinology and Metabolism, 1985
The objectives of this study were to evaluate the rate at which brown adipose tissue (BAT) from mice atrophies when its thermogenic activity is suppressed during fasting or exposure to a thermoneutral environment (33 degrees C) and whether such atrophy is accompanied by loss from BAT mitochondria of "thermogenin," the GDP binding protein associated ...
M. Desautels
semanticscholar   +3 more sources

Parallel measurements of heat production and thermogenin content in brown fat cells during cold acclimation of rats

Bioscience Reports, 1986
The maximum thermogenic capacity of brown fat cells from control and cold acclimated rats was measured using a continuous-flow microcalorimetric system, The content of the 32.000 D, brown fat specific protein, thermogenin, was measured in the cells used for heat production measurements by competitive ELISA.
E. S. Hansen, J. Knudsen
semanticscholar   +3 more sources

Brown adipocytes differentiated in vitro can express the gene for the uncoupling protein thermogenin: effects of hypothyroidism and norepinephrine.

Experimental Cell Research, 1989
Expression of the gene for the brown-fat specific uncoupling protein thermogenin was investigated in cell cultures by hybridization of isolated RNA with a cDNA clone corresponding to mouse thermogenin. The RNA was isolated 3-4 days after confluence from cells differentiated in culture from precursors isolated from the interscapular brown adipose tissue
S. Rehnmark, Jan Kopecký, Jan Kopecký, A. Jacobsson, Myriam Néchad, Myriam Néchad, D. Herron, B. D. Nelson, María Jesús Obregón, J. Nedergaard, B. Cannon   +10 more
semanticscholar   +5 more sources

Limitations in the use of the enzyme-linked immunosorbent assay (ELISA) for identification and quantification of thermogenin.

Journal of Immunological Methods, 1985
The use of immunological assays, ELISA and RIA, for the identification and quantification of thermogenin (the brown adipose tissue-specific, GDP-binding, 32 kDa uncoupling protein) raises doubts regarding the exclusive occurrence of thermogenin in brown adipose tissue.
E. S. Hansen, J. Knudsen
semanticscholar   +3 more sources

Intake of sweeteners and pungent ingredients increases the thermogenin content in brown adipose tissue of rat

Journal of Agricultural and Food Chemistry, 1991
T. Kawada, S. Sakabe, N. Aoki, Tatsuo Watanabe, Keiko Higeta, K. Iwai, E. Sugimoto   +6 more
semanticscholar   +2 more sources

Parallel increases in amount of (3H)GDP binding and thermogenin antigen in brown-adipose-tissue mitochondria of cafeteria-fed rats.

Biochemical and Biophysical Research Communications, 1984
In order to investigate the possible existence of a 'masked' (i.e. non-GDP-binding) form of thermogenin (the brown-adipose-tissue specific, 32 000 Da so-called "uncoupling" protein), rats were fed a routine pellet diet or, in addition to this, a cafeteria diet. Brown-adipose-tissue mitochondria isolated from the cafeteria-fed animals showed as expected
J. Nedergaard, A. Raasmaja, Barbara Cannon   +2 more
semanticscholar   +3 more sources

Regulation of the Amount and Activity of the Uncoupling Protein Thermogenin in Brown Adipose Tissue

, 1989
The presence of the brown-fat specific, uncoupling protein thermogenin on a meeting program devoted to mitochondrial anion carriers may at first sight seem surprising. This is because, in the role normally assumed for it (depicted on Fig. 1), it does not transport anions over the mitochondrial membrane.
B. Cannon, J. Nedergaard
semanticscholar   +2 more sources

Enzyme-linked immunosorbent assay (ELISA) studies of the interaction between mammalian and avian anti-thermogenin antibodies and brown-adipose-tissue mitochondria from different species.

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1984
Three different antibody preparations, rabbit anti-hamster and rabbit anti-rat thermogenin sera and chicken anti-rat thermogenin IgG, were tested for cross-reactivity towards isolated thermogenin and BAT mitochondria from different mammalian species using an ELISA-technique.
E. S. Hansen, J. Nedergaard, Barbara Cannon, Jens Knudsen   +3 more
semanticscholar   +3 more sources