Results 31 to 40 of about 2,424 (164)

Cryo-EM single particle analysis with the Volta phase plate

eLife, 2016
We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data.
Radostin Danev, Wolfgang Baumeister
doaj   +1 more source

Statistical Optimization of Bioleaching for Simultaneous Recovery of Cu, Sn, Pb, and Zn from Computer‐Printed Circuit Boards

Journal of Chemistry, Volume 2023, Issue 1, 2023., 2023
Electronic garbage is one of the fastest‐growing waste streams. Its disposal and appropriate management are a worldwide concern. Printed circuit boards (PCBs) are critical components in contemporary electronic gadgets that contain toxic elements. Bioprocessing of PCBs for metal recovery employing microbial methods has evolved as a green solution in ...
Murugesan Manikkampatti Palanisamy, Venkata Ratnam Myneni, Akilamudhan Palaniappan, Padmapriya Veerappan, Minar Mohamed Lebbai, G. Kavitha, Deniz Şahin   +6 more
wiley   +1 more source

The Functional State of <i>Thermoplasma acidophilum</i> Pyruvate Kinase Relies on an Extra Carboxyl-Terminal Sequence. [PDF]

Int J Mol Sci
Phylogenetic studies of the pyruvate kinase family reveal two clusters: the K+-dependent and -independent enzymes. Thermoplasma acidophilum pyruvate kinase belongs to the latter but possesses the conserved signature of those K+-dependent.
Ramírez-Silva L, Riveros-Rosas H, Hernández-Alcántara G, García-Trejo JJ, Vega-Segura A, González-Andrade M, Díaz-Salazar AJ, Salcedo-Barrientos G.   +7 more
europepmc   +2 more sources

Crystal structure of Thermus thermophilus methylenetetrahydrofolate dehydrogenase and determinants of thermostability.

PLoS ONE, 2020
The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus ...
Fernando Maiello, Gloria Gallo, Camila Coelho, Fernanda Sucharski, Leon Hardy, Martin Würtele   +5 more
doaj   +1 more source

Thermoplasma acidophilum TAA43 is an archaeal member of the eukaryotic meiotic branch of AAA ATPases [PDF]

, 2004
Sequencing of the Thermoplasma acidophilum genome revealed a new gene, taa43 , which codes for a 43-kDa protein containing one AAA domain; we therefore termed it Thermoplasma AAA ATPase of 43 kDa (TAA43). Close homologs of TAA43 are found only in related
Jürgen Peters, Lupas, A. ; https://orcid.org/, Tancred Frickey, Wolfgang Baumeister, Zwickl, P., Peter Zwickl, Baumeister, W., Santos, L., Frickey, T., Lupas, Andrei, Andrei Lupas, Lupas, A., Frickey, Tankred, Peters, J., Leticia Santos   +14 more
core   +2 more sources

Structure of a AAA+ unfoldase in the process of unfolding substrate

eLife, 2017
AAA+ unfoldases are thought to unfold substrate through the central pore of their hexameric structures, but how this process occurs is not known.
Zev A Ripstein, Rui Huang, Rafal Augustyniak, Lewis E Kay, John L Rubinstein   +4 more
doaj   +1 more source

Thermo Sequenase™ DNA Polymerase and T. acidophilum Pyrophosphatase: New Thermostable Enzymes for DNA Sequencing

BioTechniques, 1997
A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase™ DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold ...
P.B. Vander Horn, M.C. Davis, J.J. Cunniff, C. Ruan, B.F. McArdle, S.B. Samols, J. Szasz, G. Hu, K.M. Hujer, S.T. Domke, S.R. Brummet, R.B. Moffett, C.W. Fuller   +12 more
doaj   +1 more source

A broad specificity nucleoside kinase from Thermoplasma acidophilum. [PDF]

Proteins, 2013
AbstractThe crystal structure of Ta0880, determined at 1.91 Å resolution, from Thermoplasma acidophilum revealed a dimer with each monomer composed of an α/β/α sandwich domain and a smaller lid domain. The overall fold belongs to the PfkB family of carbohydrate kinases (a family member of the Ribokinase clan) which include ribokinases, 1 ...
Elkin SR, Kumar A, Price CW, Columbus L.
europepmc   +4 more sources

Structure of thermoplasmaquinone from Thermoplasma acidophilum [PDF]

FEMS Microbiology Letters, 1985
The structure of the methyl-substituted menaquinone (designated thermoplasmaquinone) from the extremely thermophilic and acidophilic archaebacterium Thermoplasma acidophilum was investigated by mass spectrometry and proton nuclear magnetic resonance spectrometry. The results of the present study show that the novel quinone from T.
openaire   +1 more source

Characterization of Thermoplasma acidophilum Deoxyribonucleic Acid [PDF]

International Journal of Systematic Bacteriology, 1975
The deoxyribonucleic acid (DNA) of the mycoplasma Thermoplasma acidophilum has a base composition of 46 mol% guanine plus cytosine (G+C). Depurination can occur to the DNA in the culture conditions, giving rise to a product of lower buoyant density. This effect probably accounts for earlier descriptions of a much lower G+C content in the DNA.
D. G. SEARCY, E. K. DOYLE
openaire   +1 more source