Flagellation and swimming motility of Thermoplasma acidophilum [PDF]
Electron microscopy of thin sections of Thermoplasma acidophilum confirmed previous observations of the absence of a typical cell wall in this organism. Negatively stained specimens revealed the almost consistent occurrence in both strains examined of monotrichously arranged flagella, about 9 micrometer long, which describe a sinuous curve with a ...
F T, Black +3 more
openaire +2 more sources
Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum. [PDF]
The metabolic enzyme transaldolase from Thermoplasma acidophilum was recombinantly expressed in Escherichia coli and could be crystallized in two polymorphic forms.
Lehwess-Litzmann A +4 more
europepmc +2 more sources
Ultrastructure of lipopolysaccharide isolated from Thermoplasma acidophilum [PDF]
The fine structure of lipopolysaccharide isolated from Thermoplasma acidophilum was examined by electron microscopy. Negative staining of the lipopolysaccharide revealed long, ribbon-like structures with some branching. The average width of the lipopolysaccharide ribbons was 5 nm.
K J, Mayberry-Carson +2 more
openaire +2 more sources
Refolding of a thermostable glyceraldehyde dehydrogenase for application in synthetic cascade biomanufacturing. [PDF]
The production of chemicals from renewable resources is gaining importance in the light of limited fossil resources. One promising alternative to widespread fermentation based methods used here is Synthetic Cascade Biomanufacturing, the application of ...
Fabian Steffler, Volker Sieber
doaj +1 more source
Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia
Archaea is an organisme with unique feature because of its ability to inhabit an extremophyle conditions. Our expeditions to Tangkuban Perahu, West Java aimed to obtain archaealstrains from the solfatara fields located in Domas crater.
AMARILA MALIK +8 more
doaj +1 more source
Impact of age-associated cyclopurine lesions on DNA repair helicases. [PDF]
8,5' cyclopurine deoxynucleosides (cPu) are locally distorting DNA base lesions corrected by nucleotide excision repair (NER) and proposed to play a role in neurodegeneration prevalent in genetically defined Xeroderma pigmentosum (XP) patients.
Irfan Khan +7 more
doaj +1 more source
Surfaces Coated with Polymer Brushes Work as Carriers for Histidine Ammonia Lyase
Immobilizing enzymes with preserved activity on solid support remains challenging. Herein, planar and colloidal silica surfaces are equipped with poly(2‐(diethylamino)ethyl methacrylate) brushes either via the “grafting‐to” or “grafting‐from” method. Histidine ammonia lyase with preserved activity is subsequently immobilized in these polymer brushes ...
Thaís Marcelino +9 more
wiley +1 more source
Evolutionary patterns of archaea predominant in acidic environment
Background Archaea of the order Thermoplasmatales are widely distributed in natural acidic areas and are amongst the most acidophilic prokaryotic organisms known so far.
Rafael Bargiela +6 more
doaj +1 more source
Crystal structure of a GCN5‐related N‐acetyltransferase from Lactobacillus curiae
The 3D structure of a GCN5‐related N‐acetyltransferase enzyme that is selective for canavanine has been elucidated and shown to share the fold and catalytic mechanism of the polyamine acetyltransferase subclass.Members of the GCN5‐related N‐acetyltransferase (GNAT) family are found in all domains of life and are involved in processes ranging from ...
Jennifer R. Fleming +3 more
wiley +1 more source
Cryo-EM single particle analysis with the Volta phase plate
We present a method for in-focus data acquisition with a phase plate that enables near-atomic resolution single particle reconstructions. Accurate focusing is the determining factor for obtaining high quality data.
Radostin Danev, Wolfgang Baumeister
doaj +1 more source

