Results 41 to 50 of about 2,424 (164)

Crystal Structure of a Nicotinate Phosphoribosyltransferase from Thermoplasma acidophilum [PDF]

Journal of Biological Chemistry, 2005
We have determined the crystal structure of nicotinate phosphoribosyltransferase from Themoplasma acidophilum (TaNAPRTase). The TaNAPRTase has three domains, an N-terminal domain, a central functional domain, and a unique C-terminal domain. The crystal structure revealed that the functional domain has a type II phosphoribosyltransferase fold that may ...
Dong Hae, Shin, Natalia, Oganesyan, Jaru, Jancarik, Hisao, Yokota, Rosalind, Kim, Sung-Hou, Kim   +5 more
openaire   +2 more sources

Proteomics Analysis of Thermoplasma acidophilum with a Focus on Protein Complexes [PDF]

Molecular & Cellular Proteomics, 2007
Two-dimensional gel electrophoresis (2DE) and MALDI-TOF MS were used to obtain a global view of the cytoplasmic proteins expressed by Thermoplasma acidophilum. In addition, glycerol gradient ultracentrifugation coupled to 2DE-MALDI-TOF MS analysis was used to identify subunits of macromolecular complexes.
Na, Sun, Florian, Beck, Roland Wilhelm, Knispel, Frank, Siedler, Beatrix, Scheffer, Stephan, Nickell, Wolfgang, Baumeister, István, Nagy   +7 more
openaire   +2 more sources

Crystallization and preliminary X-ray diffraction analysis of transaldolase from Thermoplasma acidophilum. [PDF]

Acta Crystallogr Sect F Struct Biol Cryst Commun, 2011
The metabolic enzyme transaldolase from Thermoplasma acidophilum was recombinantly expressed in Escherichia coli and could be crystallized in two polymorphic forms.
Lehwess-Litzmann A, Neumann P, Golbik R, Parthier C, Tittmann K.   +4 more
europepmc   +2 more sources

Quinone Profiles of Thermoplasma acidophilum HO-62 [PDF]

Journal of Bacteriology, 2001
ABSTRACT Quinones of Thermoplasma acidophilum HO-62 were analyzed by high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance. Menaquinone, methionaquinone, and 2- trans and 2- cis forms of thermoplasmaquinone were identified.
H, Shimada, Y, Shida, N, Nemoto, T, Oshima, A, Yamagishi   +4 more
openaire   +2 more sources

Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid

Frontiers in Bioengineering and Biotechnology, 2019
The coffee industry produces over 10 billion kg beans per year and generates high amounts of different waste products. Spent coffee grounds (SCG) are an industrially underutilized waste resource, which is rich in the polysaccharide galactomannan, a ...
Dominik Kopp, Robert D. Willows, Anwar Sunna, Anwar Sunna   +3 more
doaj   +1 more source

The Physical, Chemical, and the Biological Stability Test on Liposome EPC-TEL 2.5 as the Newest Drug Delivery Systems (Drug Carrier), In Vitro and In Vivo

Makara Journal of Health Research, 2010
This experiment is carried out in order to improve the stability of the Liposome EPC-TEL 2.5 physically, chemically, and biologically. As a new formula, this liposome that has contained phosphatidylcholine from egg yolk=EPC and Tetra-ether Lipid (TEL ...
Ernie Purwaningsih, Wawaimuli Arozal, Sri Widia A. Jusman   +2 more
doaj   +1 more source

Purification and properties of pyruvate kinase fromThermoplasma acidophilum [PDF]

FEMS Microbiology Letters, 1992
Thermoplasma acidophilum is a thermoacidophilic archaebacterium occupying a paradoxical place in phylogenetic trees (phenotypically it is a thermoacidophile but phylogenetically it classifies with the methanogens). To better understand its phylogeny, the pyruvate kinase from this organism is being investigated as a molecular marker. The enzyme has been
S, Potter, L A, Fothergill-Gilmore
openaire   +2 more sources

Architectures of archaeal GINS complexes, essential DNA replication initiation factors

BMC Biology, 2011
Background In the early stage of eukaryotic DNA replication, the template DNA is unwound by the MCM helicase, which is activated by forming a complex with the Cdc45 and GINS proteins.
Saito Mihoko, Nagasawa Naoko, Mayanagi Kouta, Shirai Tsuyoshi, Ogino Hiromi, Fujino Seiji, Ishino Sonoko, Oyama Takuji, Ishino Yoshizumi, Morikawa Kosuke   +9 more
doaj   +1 more source

Phylogenomic analysis of proteins that are distinctive of Archaea and its main subgroups and the origin of methanogenesis

BMC Genomics, 2007
Background The Archaea are highly diverse in terms of their physiology, metabolism and ecology. Presently, very few molecular characteristics are known that are uniquely shared by either all archaea or the different main groups within archaea.
Gupta Radhey S, Gao Beile
doaj   +1 more source

Enzymatic Synthesis of NAD+

Current Protocols, Volume 6, Issue 6, June 2026.
Abstract Nicotinamide adenine dinucleotide (NAD+) is an essential metabolite involved in numerous biological processes, functioning both as a redox cofactor in cellular energy metabolism and as a co‐substrate for NAD+‐utilizing enzymes, including sirtuins and poly(ADP‐ribose) polymerases.
Emily Boyle, Olivia Zhang, Yana Cen
wiley   +1 more source