Results 41 to 50 of about 4,201 (187)

Characterization of the Cdc6 Homologues from the Euryarchaeon Thermoplasma acidophilum. [PDF]

Open Biochem J, 2008
Archaeal cell division cycle protein 6 (Cdc6) homologues are thought to be involved in the initiation process of DNA replication. In the present study, a biochemical characterization of the two Cdc6 proteins from the archaeon Thermoplasma acidophilum has been performed.
Haugland GT, Innselset M, Madern D, Birkeland NK.   +3 more
europepmc   +4 more sources

Discovery of a novel transcriptional regulator of sugar catabolism in archaea

Molecular Microbiology, Volume 120, Issue 2, Page 224-240, August 2023., 2023
A novel transcriptional regulator, GfcR, has been identified that activates the degradation pathways of glucose, fructose, galactose and glycerol in the archaeon Haloferax volcanii. GfcR comprises a phosphoribosyltransferase domain with an N‐terminal helix‐turn‐helix DNA‐binding motif.
Ulrike Johnsen, Marius Ortjohann, Andreas Reinhardt, Jonathan M. Turner, Caleb Stratton, Katherine R. Weber, Karol M. Sanchez, Julie Maupin‐Furlow, Christopher Davies, Peter Schönheit   +9 more
wiley   +1 more source

Thermoplasma acidophilum TAA43 is an archaeal member of the eukaryotic meiotic branch of AAA ATPases [PDF]

, 2004
Sequencing of the Thermoplasma acidophilum genome revealed a new gene, taa43 , which codes for a 43-kDa protein containing one AAA domain; we therefore termed it Thermoplasma AAA ATPase of 43 kDa (TAA43). Close homologs of TAA43 are found only in related
Jürgen Peters, Lupas, A. ; https://orcid.org/, Tancred Frickey, Wolfgang Baumeister, Zwickl, P., Peter Zwickl, Baumeister, W., Santos, L., Frickey, T., Lupas, Andrei, Andrei Lupas, Peters, J., Leticia Santos, Frickey, Tancred, Baumeister, Wolfgang, Peters, Jürgen, Santos Martínez, Martha Leticia, Zwickl, Peter   +17 more
core   +2 more sources

Crystal structure of Thermus thermophilus methylenetetrahydrofolate dehydrogenase and determinants of thermostability.

PLoS ONE, 2020
The elucidation of mechanisms behind the thermostability of proteins is extremely important both from the theoretical and applied perspective. Here we report the crystal structure of methylenetetrahydrofolate dehydrogenase (MTHFD) from Thermus ...
Fernando Maiello, Gloria Gallo, Camila Coelho, Fernanda Sucharski, Leon Hardy, Martin Würtele   +5 more
doaj   +1 more source

Ubiquitin found in the archaebacterium Thermoplasma acidophilum

FEBS Letters, 1993
Systematic N‐terminal sequencing of the low molecular weight proteins from Thermoplasma acidophilum separated by two‐dimensional polyacrylamide get electrophoresis led to the discovery of a polypeptide with an apparent M r of 4.5 kDa identical as its first 18 amino acid residues to human ubiquitin.
Wolf, S., Lottspeich, F., Baumeister, W.
openaire   +3 more sources

Structure of a AAA+ unfoldase in the process of unfolding substrate

eLife, 2017
AAA+ unfoldases are thought to unfold substrate through the central pore of their hexameric structures, but how this process occurs is not known.
Zev A Ripstein, Rui Huang, Rafal Augustyniak, Lewis E Kay, John L Rubinstein   +4 more
doaj   +1 more source

Thermo Sequenase™ DNA Polymerase and T. acidophilum Pyrophosphatase: New Thermostable Enzymes for DNA Sequencing

BioTechniques, 1997
A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase™ DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold ...
P.B. Vander Horn, M.C. Davis, J.J. Cunniff, C. Ruan, B.F. McArdle, S.B. Samols, J. Szasz, G. Hu, K.M. Hujer, S.T. Domke, S.R. Brummet, R.B. Moffett, C.W. Fuller   +12 more
doaj   +1 more source

Characterization of Thermoplasma acidophilum Deoxyribonucleic Acid [PDF]

International Journal of Systematic Bacteriology, 1975
The deoxyribonucleic acid (DNA) of the mycoplasma Thermoplasma acidophilum has a base composition of 46 mol% guanine plus cytosine (G+C). Depurination can occur to the DNA in the culture conditions, giving rise to a product of lower buoyant density. This effect probably accounts for earlier descriptions of a much lower G+C content in the DNA.
D. G. SEARCY, E. K. DOYLE
openaire   +1 more source

Crystal Structure of a Nicotinate Phosphoribosyltransferase from Thermoplasma acidophilum [PDF]

Journal of Biological Chemistry, 2005
We have determined the crystal structure of nicotinate phosphoribosyltransferase from Themoplasma acidophilum (TaNAPRTase). The TaNAPRTase has three domains, an N-terminal domain, a central functional domain, and a unique C-terminal domain. The crystal structure revealed that the functional domain has a type II phosphoribosyltransferase fold that may ...
Dong Hae, Shin, Natalia, Oganesyan, Jaru, Jancarik, Hisao, Yokota, Rosalind, Kim, Sung-Hou, Kim   +5 more
openaire   +2 more sources

Structure of thermoplasmaquinone from Thermoplasma acidophilum [PDF]

FEMS Microbiology Letters, 1985
The structure of the methyl-substituted menaquinone (designated thermoplasmaquinone) from the extremely thermophilic and acidophilic archaebacterium Thermoplasma acidophilum was investigated by mass spectrometry and proton nuclear magnetic resonance spectrometry. The results of the present study show that the novel quinone from T.
openaire   +1 more source