Results 61 to 70 of about 2,424 (164)

Domain swapping: a mathematical model for quantitative assessment of structural effects

FEBS Open Bio, Volume 14, Issue 12, Page 2006-2025, December 2024.
The structure of a single chain interpreted on the basis of the hydrophobicity distribution (red line on profiles) represents the status with respect to an ideal hydrophobic core (blue) which is represented by a modified idealised hydrophobicity distribution (green line, upper part).
Irena Roterman, Katarzyna Stapor, Dawid Dułak, Leszek Konieczny   +3 more
wiley   +1 more source

Metal ion activation and DNA recognition by the Deinococcus radiodurans manganese sensor DR2539

The FEBS Journal, Volume 291, Issue 15, Page 3384-3402, August 2024.
The manganese sensor DR2539 from Deinococus radiodurans recognises the dr1709 promoter region, which consists of a 21 base pair pseudo‐palindrome binding two DR2539 monomers. Here, we identified four manganese binding sites and investigated protein‐DNA interactions, with critical nucleotides being probed.
Cristiano Mota, Myles Webster, Melissa Saidi, Ulrike Kapp, Chloe Zubieta, Gabriele Giachin, José Antonio Manso, Daniele de Sanctis   +7 more
wiley   +1 more source

Development of a Highly Selective NAD+‐Dependent Glyceraldehyde Dehydrogenase and its Application in Minimal Cell‐Free Enzyme Cascades

ChemSusChem, Volume 17, Issue 4, February 22, 2024.
This work demonstrates the power of enzyme engineering to tailor a biocatalyst to meet various requirements of a cell‐free artificial multi‐enzyme cascade. We targeted glyceraldehyde dehydrogenase (GADH) as a key enzyme to efficiently produce ethanol from D‐glucose (obtainable from lignocellulosic agricultural wastes).
Mariko Teshima, Samuel Sutiono, Manuel Döring, Barbara Beer, Mikael Boden, Gerhard Schenk, Volker Sieber   +6 more
wiley   +1 more source

An Enzymatic Cofactor Regeneration System for the in‐Vitro Reduction of Isolated C=C Bonds by Geranylgeranyl Reductases

ChemBioChem, Volume 25, Issue 1, January 2, 2024.
A ferredoxin from Archaeoglobus fulgidus has been identified that regenerates the cofactor in the corresponding geranylgeranyl reductase (GGR) for the reduction of isolated C=C bonds. The reaction was coupled to an unspecific ferredoxin reductase from E.
Richard Niese, Ketaki Deshpande, Michael Müller   +2 more
wiley   +1 more source

The IbeA protein from adherent invasive Escherichia coli is a flavoprotein sharing structural homology with FAD‐dependent oxidoreductases

The FEBS Journal, Volume 291, Issue 1, Page 177-203, January 2024.
Invasion of brain endothelium protein A (IbeA), a virulence factor specific to pathogenic Escherichia coli, plays an important role in the invasion of host cells by the intestinal adherent invasive Escherichia coli strain involved in Crohn's disease and in the survival of these bacteria within host macrophages.
Théo Paris, Agneta Kiss, Luca Signor, Georges Lutfalla, Mickaël Blaise, Elisabetta Boeri Erba, Laurent Chaloin, Laure Yatime   +7 more
wiley   +1 more source

Insights Into the Chromatin Structure of Thermoplasma volcanium: Archaeal HU Regulates Alba‐Mediated DNA Compaction

Archaea, Volume 2024, Issue 1, 2024.
Archaeal species encode a diversity of chromatin proteins that play distinct roles in genome compaction. Although our understanding of the individual proteins has been growing, their contributions to higher‐order folding in the archaeal chromosome remain to be explored. In this study, we investigated the chromatin structure of Thermoplasma volcanium, a
Kirk Amerigo B. Aycardo, Eloise I. Prieto, Stefan Spring   +2 more
wiley   +1 more source

Ultrastructural localization of Thermoplasma acidophilum surface carbohydrate by using concanavalin A [PDF]

Journal of Bacteriology, 1978
Surface carbohydrate, presumably the lipopolysaccharide, of Thermoplasma acidophilum was visualized by means of the concanavalin A, horseradish peroxidase, and diaminobenzidine cytochemical staining procedure.
K J, Mayberry-Carson, M J, Jewell, P F, Smith   +2 more
openaire   +2 more sources

Lipoylation and assembly of a 2-oxoacid dehydrogenase multienzyme complex from thermoplasma acidophilum [PDF]

, 2009
Energy generating processes like the citric acid cycle are a pivotal part of metabolism. Members of the 2-oxoacid dehydrogenase multienzyme complex (OADHC) superfamily feed into and act within the citric acid cycle.
Posner, Mareike
core  

Physicochemical Characterization of Tetraetherlipids from Thermoplasma acidophilum

Zeitschrift für Naturforschung C, 1985
Abstract By means of differential thermoanalysis, the miscibility of the main polar tetraether lipid of Thermoplasma acidophilum with two ester lipids, dipalmitoyl phosphatidylcholine and dipalmitoyl phosphatidylglycerol, resp., in the presence of excess water was studied. It is shown that with increasing fraction of tetraether lipid in
Blöcher, Detlef, Gutermann, Raymund, Henkel, Birgit, Ring, Klaus   +3 more
openaire   +2 more sources

Charakterisierung des Proteoms und des Transkriptoms von Thermoplasma acidophilum

, 2009
In this study, proteomics and transcriptomics studies of Thermoplasma acidophilum were performed. Mass spectrometry-based proteomics approaches combined with different pre-separation methods were used, to provide a global overview of the cytoplasmic ...
Sun, Na
core