Results 71 to 80 of about 4,201 (187)

Parallelizing the cryo‐EM structure determination in THUNDER using GPU cluster

Engineering Reports, Volume 7, Issue 1, January 2025.
We designed a new computing approach specialized based on the 3D reconstruction algorithm of THUNDER for GPU to optimize and maximize the computing power of a single GPU, multiple GPU, and the GPU cluster, highlighted by a well‐designed cache structure and mixed computing precision of single‐precision and double‐precision.
Zhao Wang, Huabin Ruan, Guangwen Yang, Xueming Li   +3 more
wiley   +1 more source

Localization of subunits in proteasomes fromThermoplasma acidophilumby immunoelectron microscopy

, 1991
The subunit topography of the Thermoplasma acidophilum proteasome was determined by immunoelectron microscopy using monospecific antibodies directed against the two constituent subunits (alpha,beta).
Burkhardt Dahlmann, Grziwa, A., Dahlmann, B., Anja Grziwa, Wolfgang Baumeister, Baumeister, W., Kopp, F., Friedrich Kopp   +7 more
core   +1 more source

The Thermosome of Thermoplasma acidophilum and Its Relationship to the Eukaryotic Chaperonin TRiC [PDF]

European Journal of Biochemistry, 1995
A high molecular‐mass protein complex from the archaebacterium Thermoplasma acidophilum, referred to here as the ‘thermosome’, is built from two subunits (Mr 58 and 60). The thermosome has been purified to homogeneity. The molecular mass of the native complex was determined to be 1061 ± 30 Da by scanning transmission electron microscopy.
Waldmann, T., Nimmesgern, E., Nitsch, M., Peters, J., Pfeifer, G., Müller, S., Kellermann, J., Engel, A., Hartl, F., Baumeister, W.   +9 more
openaire   +3 more sources

Extremophilic Microorganisms as a Source of Emerging Enzymes for the Food Industry: A Review

Food Science &Nutrition, Volume 13, Issue 1, January 2025.
Enzymes such as amylases, lipases, proteases, xylanases, laccases, pullulanase, chitinases, pectinases, esterases, isomerases, and dehydrogenases could be derived from extremophilic organisms like thermophiles, psychrophiles, acidophiles, alkaliphiles, and halophiles. These enzymes or extremozymes are applicable in the production of safe foods (such as
Tolulope Joshua Ashaolu, Tanu Malik, Rakhi Soni, Miguel A. Prieto, Seid Mahdi Jafari   +4 more
wiley   +1 more source

Thermoplasma acidophilum Cdc6 protein stimulates MCM helicase activity by regulating its ATPase activity. [PDF]

Nucleic Acids Res, 2008
The minichromosome maintenance (MCM) proteins are thought to function as the replicative helicases in archaea. In most archaeal species studied, the interaction between MCM and the initiator protein, Cdc6, inhibits helicase activity.
Haugland GT, Sakakibara N, Pey AL, Rollor CR, Birkeland NK, Kelman Z.   +5 more
europepmc   +2 more sources

Domain swapping: a mathematical model for quantitative assessment of structural effects

FEBS Open Bio, Volume 14, Issue 12, Page 2006-2025, December 2024.
The structure of a single chain interpreted on the basis of the hydrophobicity distribution (red line on profiles) represents the status with respect to an ideal hydrophobic core (blue) which is represented by a modified idealised hydrophobicity distribution (green line, upper part).
Irena Roterman, Katarzyna Stapor, Dawid Dułak, Leszek Konieczny   +3 more
wiley   +1 more source

Metal ion activation and DNA recognition by the Deinococcus radiodurans manganese sensor DR2539

The FEBS Journal, Volume 291, Issue 15, Page 3384-3402, August 2024.
The manganese sensor DR2539 from Deinococus radiodurans recognises the dr1709 promoter region, which consists of a 21 base pair pseudo‐palindrome binding two DR2539 monomers. Here, we identified four manganese binding sites and investigated protein‐DNA interactions, with critical nucleotides being probed.
Cristiano Mota, Myles Webster, Melissa Saidi, Ulrike Kapp, Chloe Zubieta, Gabriele Giachin, José Antonio Manso, Daniele de Sanctis   +7 more
wiley   +1 more source

Dolichyl-phosphomannose synthase from the Archae Thermoplasma acidophilum [PDF]

Glycobiology, 1996
Archae (formerly Archaebacteria) comprise an entire kingdom of organisms placed halfway between prokaryotes and eucaryotes in evolution. This class of organisms lacks murein cell wall and is devoid of organelles, yet Archae synthesize and export N-linked and O-linked glycoproteins utilizing only the plasma membrane.
B C, Zhu, R A, Laine
openaire   +2 more sources

Development of a Highly Selective NAD+‐Dependent Glyceraldehyde Dehydrogenase and its Application in Minimal Cell‐Free Enzyme Cascades

ChemSusChem, Volume 17, Issue 4, February 22, 2024.
This work demonstrates the power of enzyme engineering to tailor a biocatalyst to meet various requirements of a cell‐free artificial multi‐enzyme cascade. We targeted glyceraldehyde dehydrogenase (GADH) as a key enzyme to efficiently produce ethanol from D‐glucose (obtainable from lignocellulosic agricultural wastes).
Mariko Teshima, Samuel Sutiono, Manuel Döring, Barbara Beer, Mikael Boden, Gerhard Schenk, Volker Sieber   +6 more
wiley   +1 more source

Identification and characterization of Thermoplasma acidophilum 2-keto-3-deoxy-D-gluconate kinase: A new class of sugar kinases

, 2019
The thermoacidophilic archaeon Thermoplasma acidophilum has long been known to utilize D-glucose via the non-phosphorylated Entner-Doudoroff (nED) pathway. We now report the identification of a gene encoding 2-keto-3-deoxy-D-gluconate (KDG) kinase.
Jung, JH, Lee, SB
core   +1 more source