Results 11 to 20 of about 1,447 (168)

Identification of Novel Thermosensors in Gram-Positive Pathogens [PDF]

Frontiers in Molecular Biosciences, 2020
Temperature is a crucial variable that every living organism, from bacteria to humans, need to sense and respond to in order to adapt and survive. In particular, pathogenic bacteria exploit host-temperature sensing as a cue for triggering virulence gene ...
Pilar Fernández, Alejandra Raquel Díaz, María Florencia Ré, Lucía Porrini, Diego de Mendoza, Diego de Mendoza, Daniela Albanesi, Daniela Albanesi, María Cecilia Mansilla, María Cecilia Mansilla   +9 more
doaj   +3 more sources

Development of a Temperature Senor Based on 19F-labeled Phosphorylated Ubiquitin

Chinese Journal of Magnetic Resonance, 2021
Ubiquitin (Ub) is a signaling protein in eukaryotic cells, and it functions in the processes of protein degradation and DNA repairing. The published data indicated that the phosphorylation of Ub on Ser65 leads to two stable solution conformations, which ...
Zhi-wu ZHANG, Ju YANG, Ze-feng NIE, Shang-xiang YE, Xu DONG, Chun TANG   +5 more
doaj   +1 more source

OmpA, a Common Virulence Factor, Is Under RNA Thermometer Control in Yersinia pseudotuberculosis

Frontiers in Microbiology, 2021
The outer membrane protein OmpA is a virulence factor in many mammalian pathogens. In previous global RNA structure probing studies, we found evidence for a temperature-modulated RNA structure in the 5'-untranslated region (5'-UTR) of the Yersinia ...
Daniel Scheller, Christian Twittenhoff, Franziska Becker, Marcel Holler, Franz Narberhaus   +4 more
doaj   +1 more source

Advances in the nanostructure characterization of biological hydrogels formed by the prion-like domain of EARLY FLOWERING 3. [PDF]

Acta Crystallogr D Struct Biol
In biological systems, hydrogels often arise through liquid–liquid phase separation, where biomolecular condensates can age into gel‐like states. SAXS is a key technique to probe the molecular organization of these samples. This study introduces an in‐vacuum Gel‐Cell for SAXS‐based structural analysis of a biologically derived hydrogel formed by the ...
Hutin S, Filho PLO, Popov AM, Pernot P, Zubieta C, Tully MD.   +5 more
europepmc   +2 more sources

Phosphorylation activates the yeast small heat shock protein Hsp26 by weakening domain contacts in the oligomer ensemble

Nature Communications, 2021
Small heat shock proteins (sHsps) form large spherical assemblies and their regulation is not well understood. Here, the authors provide insights into the mechanism of Hsp26 activation by characterising phospho-mimetic mutants of yeast Hsp26.
Moritz Mühlhofer, Carsten Peters, Thomas Kriehuber, Marina Kreuzeder, Pamina Kazman, Natalia Rodina, Bernd Reif, Martin Haslbeck, Sevil Weinkauf, Johannes Buchner   +9 more
doaj   +1 more source

A Prion-based Thermosensor in Plants [PDF]

Molecular Cell, 2020
Some prion-like domains and low-complexity regions provide the multivalency required to facilitate protein phase separation to regulate protein function. Jung et al. (2020) demonstrate how natural selection of the ELF3 prion-like domain gives rise to an intuitive biological switch that directly responds to temperature.
Edward G, Wilkinson, Lucia C, Strader
openaire   +2 more sources

Recent Synthetic Biology Approaches for Temperature- and Light-Controlled Gene Expression in Bacterial Hosts

Molecules, 2022
The expression of genes of interest (GOI) can be initiated by providing external stimuli such as temperature shifts and light irradiation. The application of thermal or light stimuli triggers structural changes in stimuli-sensitive biomolecules within ...
Jongdoo Choi, Jiyeun Ahn, Jieun Bae, Minseob Koh   +3 more
doaj   +1 more source

Phase Separation as a Molecular Thermosensor [PDF]

Developmental Cell, 2020
How organisms sense temperature is a long-standing question. However, the identification of molecular thermosensors has been limited. Now, in a recent issue of Nature, Jung et al. demonstrate that phase separation of ELF3, a component of the circadian clock, acts as a thermosensor.
Changxuan, Li, Xiaofeng, Fang
openaire   +2 more sources

RNA structure mediated thermoregulation: What can we learn from plants?

Frontiers in Plant Science, 2022
RNA molecules have the capacity to form a multitude of distinct secondary and tertiary structures, but only the most energetically favorable conformations are adopted at any given time. Formation of such structures strongly depends on the environment and
Sherine E. Thomas, Martin Balcerowicz, Betty Y.-W. Chung   +2 more
doaj   +1 more source

Ecological Adaptation Mechanisms Underlying Successful Plant Reproduction. [PDF]

Adv Sci (Weinh)
During floral induction, various environmental and endogenous signals converge to regulate the florigen protein, which is transported from leaves to the SAM to initiate flowering. Within the SAM, a complex network of receptor kinases and small peptides orchestrates floral development with high spatiotemporal precision.
Zhao H, Huo S, Xing Y, Zhang S, Li G, Liu Y, Jin C, Li F, Ge X.   +8 more
europepmc   +2 more sources