Results 181 to 190 of about 13,523 (239)

Reduction of Ferricyanide by Thiamine or Thiamine Pyrophosphate

Biochemical and Biophysical Research Communications, 1996
The colorimetric assay for the activity of pyruvate dehydrogenase (EC 1.2.4.1) developed by Itokawa is based on a coupled reduction of ferricyanide and the formation of Prussian blue (Brian Research 94, 475-484). In this assay system, we found that the coenzyme, thiamine pyrophosphate, itself reduced ferricyanide independent of both the enzyme and the ...
G, Li, M E, Cook, W, Wu
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Thiamine deficiency in rats affects thiamine metabolism possibly through the formation of oxidized thiamine pyrophosphate

Biochimica et Biophysica Acta (BBA) - General Subjects, 2021
Thiamine deficiency (TD) has a number of features in common with the neurodegenerative diseases development and close relationship between TD and oxidative stress (OS) has been repeatedly reported in the literature. The aim of this study is to understand how alimentary TD, accompanied by OS, affects the expression and level of two thiamine metabolism ...
Pavlova, O, Stepanenko, S, Chehivska, L, Sambon, Margaux, Bettendorff, Lucien, Parkhomenko, Y   +5 more
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An efficient enzymatic synthesis of thiamin pyrophosphate

Bioorganic & Medicinal Chemistry Letters, 2003
Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.
Jonathan S, Melnick, K Ingrid, Sprinz, Jason J, Reddick, Cynthia, Kinsland, Tadhg P, Begley   +4 more
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Probing riboswitch–ligand interactions using thiamine pyrophosphate analogues

Organic & Biomolecular Chemistry, 2012
The Escherichia coli thiM riboswitch forms specific contacts with its natural ligand, thiamine pyrophosphate (TPP or thiamine diphosphate), allowing it to generate not only nanomolar binding affinity, but also a high degree of discrimination against similar small molecules.
Chen, Liuhong, Cressina, Elena, Dixon, Neil, Erixon, Karl, Agyei-Owusu, Kwasi, Micklefield, Jason, Smith, Alison G., Abell, Chris, Leeper, Finian J.   +8 more
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Thiamine pyrophosphate uptake into isolated rat liver mitochondria

Archives of Biochemistry and Biophysics, 1990
The fact that thiamine pyrophosphate is synthesized in cytosol necessitates its uptake into mitochondria. The ability of mitochondria to take up externally added thiamine pyrophosphate was investigated by measuring the intramitochondrial thiamine pyrophosphate content using an enzymatic method.
BARILE, Maria, PASSARELLA S., QUAGLIARIELLO E.   +2 more
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Ligand Recognition Mechanism of Thiamine Pyrophosphate Riboswitch Aptamer

Bulletin of the Korean Chemical Society, 2017
Riboswitches regulate gene expression by coupling ligand binding to a structural transition of the riboswitch, but the coupling mechanism is still controversial. We addressed this issue by characterizing both the ligand‐free state of the Escherichia coli thiamine pyrophosphate (TPP) riboswitch aptamer and its structural transition upon ligand binding ...
Uhm, H, Hohng, S
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