Results 81 to 90 of about 810,413 (280)

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Effects of artificial accelerated aging on the optical properties of resin composites

open access: yesBioscience Journal, 2018
Objective: The aim of this study was to evaluate the effects of ultraviolet artificial aging on color, fluorescence, gloss, and translucency of different composite resins.
Debora Cristina Barbosa Dantas   +4 more
doaj   +3 more sources

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

open access: yesFEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow   +12 more
wiley   +1 more source

Stochastic simulation of the cure of advanced composites [PDF]

open access: yes, 2015
This study focuses on the development of a stochastic simulation methodology to study the effects of cure kinetics uncertainty, in plane fibre misalignment and boundary conditions uncertainty on the cure process of composite materials.
Mesogitis, Tassos
core   +3 more sources

Sandwich Structured Composites for Aeronautics: Methods of Manufacturing Affecting Some Mechanical Properties

open access: yesInternational Journal of Aerospace Engineering, 2016
Sandwich panels are composites which consist of two thin laminate outer skins and lightweight (e.g., honeycomb) thick core structure. Owing to the core structure, such composites are distinguished by stiffness. Despite the thickness of the core, sandwich
Aneta Krzyżak   +5 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Effect of Using Bulk fill Composites on Fracture Resistance of Maxillary Premolars with MOD Cavities

open access: yesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, 2020
BACKGROUND AND OBJECTIVE: Bulk fill composites are an innovative class of dental resin composite materials, developed to simplify the restoration procedures, and are preferred to conventional composites if they have good mechanical properties and ...
N Shadman   +3 more
doaj  

De novo synthesis of short‐chain aldehydes and hydrocarbons secreted by the brown marmorated stink bug Halyomorpha halys

open access: yesFEBS Open Bio, EarlyView.
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

open access: yesFEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

SPG4 and Dementia: Expanding the Clinical Spectrum

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Hereditary spastic paraplegia (HSP) is a group of disorders characterized by progressive spasticity and lower limb weakness, with mutations in SPG4/SPAST being the most common cause. Detailed studies and clinical and molecular comparisons across different populations are missing.
Emanuele Panza   +19 more
wiley   +1 more source

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