Results 81 to 90 of about 2,565 (102)
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Effect of total intact glucosinolate intake from rapeseed meals with or without thioglucosidase (EC 3.2.3.1) or copper additions to the diet on the concentrations of 1-cyano-2-hydroxy-3-butene in the ileal digesta and faeces of growing pigs

Animal Feed Science and Technology, 1995
Abstract Studies were made in pigs with cannulae sited near the terminal ileum of concentrations of the nitrile 1-cyano-2-hydroxy-3-butene (CHB) in ileal digesta and faeces consequent from varying daily total intact glucosinolate intakes induced by including in the diet rapeseed meals (200 g kg −1 ) of different total intact glucosinolate ...
T.L.J. Lawrence   +3 more
exaly   +2 more sources

Solubilization and Selected Properties of Crambe Seed Thioglucosidase (Thioglucoside Glucohydrolase, EC 3.2.3.1)

Canadian Journal of Biochemistry, 1973
Ultrasonic treatment releases thioglucoside glucohydrolase (thioglucosidase) from insoluble particles of Crambe abyssinica seed meal. The crude enzyme is optimally activated by 10−2 M ascorbate, requires a reducing agent for stability, and is inhibited by 10−3 M p-chloromercuribenzenesulfonate.Chromatography of a meal extract on cross-linked dextran ...
exaly   +3 more sources

Thioglucosidase

1991
Dietmar Schomburg, Margit Salzmann
exaly   +2 more sources

Thioglucosidase activity from Sphingobacterium sp. strain OTG1

Applied Microbiology and Biotechnology, 2001
Screening for novel thioglucoside hydrolase activity resulted in the isolation of Sphingobacterium sp. strain OTG1 from enrichment cultures containing octylthioglucoside (OTG). OTG was hydrolysed into octanethiol and glucose by cell free extracts.
Meulenbeld, G.H., Hartmans, S.
openaire   +3 more sources

Gamma‐irradiation to inactivate thioglucosidase of crucifers

Journal of the American Oil Chemists' Society, 1987
AbstractThe crucifers contain glucosinolates which through enzymatic hydrolysis give rise to toxicants that limit the use of oil‐free meal obtainable from this plant family. Seeds from three crucifers were used to test gamma irradiation to inactivate enzyme systems as a step toward detoxification. Seeds ofCrambe abyssinica Hochst (crambe), ground seeds
K. J. Lessman, B. D. McCaslin
openaire   +1 more source

Effect of ascorbic acid on thioglucosidases from different crucifers

Phytochemistry, 1972
Abstract Thioglucosidase activity was demonstrated in partially-purified preparations from several Cruciferae oilseeds, both in the presence and absence of ascorbic acid. The amount of activation by ascorbic acid differed among the enzyme preparations from different species. Buffer composition and pH were found to significantly affect enzyme activity,
H.M. Henderson, T.J. McEwen
openaire   +1 more source

Purification and Characterization of Broccoli (Brassica oleracea var. italica) Myrosinase (β-Thioglucosidase Glucohydrolase)

Journal of Agricultural and Food Chemistry, 2014
Myrosinase (β-thioglucosidase glucohydrolase, EC 3.2.1.147) from broccoli (Brassica oleracea var. italica) was purified by ammonium sulfate precipitation followed by concanavalin A affinity chromatography, with an intermediate dialysis step, resulting in 88% recovery and 1318-fold purification. These are the highest values reported for the purification
Andrea, Mahn   +2 more
openaire   +3 more sources

Effect of organic reducing agents and ferrous ion on thioglucosidase activity of Crambe abyssinica seed

Canadian Journal of Biochemistry, 1970
The addition of L-ascorbate or 2-mercaptoethanol to aged crambe seed meal tends to restore the fresh meal pattern of epi-progoitrin hydrolysis to nitriles instead of (R)-goitrin. Neither of these reducing agents has an effect on the breakdown of epi-progoitrin to goitrin by an insoluble particulate thioglucosidase from crambe meal.
H L, Tookey, I A, Wolff
openaire   +2 more sources

Effects of Microwave Heating on Thioglucosidase Activity in Intact Seeds of Crambe1

Crop Science, 1979
Depodded (silicles removed) and podded seeds of ‘Meyer’ crambe (Crambe abyssinica Hochst.) were subjected to microwave (2450 MHz) heating in a Litton 500 or 850 commercial oven for periods varying from 70 to 270 sec to explore the feasibility of using microwave heating as a means of thioglucosidase inactivation.
K. J. Lessman, A. W. Kirleis
openaire   +1 more source

Glucosinolate hydrolytic products given by Sinapis alba, and Brassica napus thioglucosidases

Phytochemistry, 1974
Abstract Thioglucosidase prepared from rapeseed (Brassica napus cvs Zephyr and Bronowski) showed one major band in polyacrylamide gel and a high ...
openaire   +1 more source

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